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QXY21 NHS ester [equivalent to QSY-21 NHS ester]

QSY-21 NHS esters (or succinimidyl esters)&nbsp;readily reacts with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
QSY-21 NHS esters (or succinimidyl esters)&nbsp;readily reacts with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
QSY-21 NHS esters (or succinimidyl esters)&nbsp;readily reacts with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
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Physical properties
Molecular weight892.90
SolventDMSO
Spectral properties
Absorbance (nm)660
Correction Factor (280 nm)0.32
Extinction coefficient (cm -1 M -1)890001
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
892.90
Absorbance (nm)
660
Correction Factor (280 nm)
0.32
Extinction coefficient (cm -1 M -1)
890001
QXY21 is the same molecule to QSY-21 acid (ThermoFisher). QSY-21 has a broad and intense absorption at ~661 nm maxima with no detectable fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common long-wavelength quencher to best pair with Cy5, Alexa Fluor 647, iFluor 647 or other spectrally similar fluorescent dyes.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of QXY21 NHS ester [equivalent to QSY-21 NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM111.995 µL559.973 µL1.12 mL5.6 mL11.199 mL
5 mM22.399 µL111.995 µL223.989 µL1.12 mL2.24 mL
10 mM11.199 µL55.997 µL111.995 µL559.973 µL1.12 mL

Molarity calculator

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Spectrum


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Spectral properties

Absorbance (nm)660
Correction Factor (280 nm)0.32
Extinction coefficient (cm -1 M -1)890001

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Citations


View all 12 citations: Citation Explorer
A Sensitive Near-Infrared Fluorescent Sensor for Mitochondrial Hydrogen Sulfide
Authors: Ji, A., Fan, Y., Ren, W., Zhang, S., Ai, H. W.
Journal: ACS Sens (2018): 992-997
Imaging intracellular quantum dots: fluorescence microscopy and transmission electron microscopy
Authors: Szymanski, C. J., Yi, H., Liu, J. L., Wright, E. R., Payne, C. K.
Journal: Methods Mol Biol (2013): 21-33
Family of enhanced photoacoustic imaging agents for high-sensitivity and multiplexing studies in living mice
Authors: de la Zerda, A., Bodapati, S., Teed, R., May, S. Y., Tabakman, S. M., Liu, Z., Khuri-Yakub, B. T., Chen, X., Dai, H., Gambhir, S. S.
Journal: ACS Nano (2012): 4694-701
Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells
Authors: Takahashi, S., Piao, W., Matsumura, Y., Komatsu, T., Ueno, T., Terai, T., Kamachi, T., Kohno, M., Nagano, T., Hanaoka, K.
Journal: J Am Chem Soc (2012): 19588-91
Visualizing mechanical tension across membrane receptors with a fluorescent sensor
Authors: Stabley, D. R., Jurchenko, C., Marshall, S. S., Salaita, K. S.
Journal: Nat Methods (2011): 64-7
Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine-Quantum Dots
Authors: Jablonski, A. E., Kawakami, T., Ting, A. Y., Payne, C. K.
Journal: J Phys Chem Lett (2010): 1312-1315
A comparative study of the binding of QSY 21 and Rhodamine 6G fluorescence probes to DNA: structure and dynamics
Authors: Kabelac, M., Zim and l, F., Fessl, T., Chval, Z., Lankas, F.
Journal: Phys Chem Chem Phys (2010): 9677-84
Evaluation of glucose sensitive affinity binding assay entrapped in fluorescent dissolved-core alginate microspheres
Authors: Chaudhary, A., Raina, M., Harma, H., Hanninen, P., McShane, M. J., Srivastava, R.
Journal: Biotechnol Bioeng (2009): 1075-85
Dissolved core alginate microspheres as &quot;smart-tattoo&quot; glucose sensors
Authors: Chaudhary, A., Raina, M., McShane, M. J., Srivastava, R.
Journal: Conf Proc IEEE Eng Med Biol Soc (2009): 4098-101
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin &quot;quencher-chaser&quot;: a dual &quot;quench and chase&quot; strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa, M., Kosaka, N., Choyke, P. L., Kobayashi, H.
Journal: Bioconjug Chem (2009): 147-54

References


View all 1 references: Citation Explorer
Synthesis and characterization of a small, membrane-permeant, caspase-activatable far-red fluorescent peptide for imaging apoptosis
Authors: Bullok K, Piwnica-Worms D.
Journal: J Med Chem (2005): 5404