Calculator
QXY21 [equivalent to QSY-21]
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 718.27 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 660 |
| Correction Factor (280 nm) | 0.32 |
| Extinction coefficient (cm -1 M -1) | 890001 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
Molecular weight 718.27 | Absorbance (nm) 660 | Correction Factor (280 nm) 0.32 | Extinction coefficient (cm -1 M -1) 890001 |
QXY21 is the same molecule to QSY-21 acid (ThermoFisher). QSY-21 has a broad and intense absorption at ~661 nm maxima with no detectable fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common long-wavelength quencher to best pair with Cy5, Alexa Fluor 647, iFluor 647 or other spectrally similar fluorescent dyes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of QXY21 [equivalent to QSY-21] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 139.223 µL | 696.117 µL | 1.392 mL | 6.961 mL | 13.922 mL |
| 5 mM | 27.845 µL | 139.223 µL | 278.447 µL | 1.392 mL | 2.784 mL |
| 10 mM | 13.922 µL | 69.612 µL | 139.223 µL | 696.117 µL | 1.392 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 660 |
| Correction Factor (280 nm) | 0.32 |
| Extinction coefficient (cm -1 M -1) | 890001 |
Images
Figure 1. In the presence of an activating agent (e.g., EDC, DCC etc) QSY-21 acid reacts with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Figure 2. Chemical structure for QXY21 [equivalent to QSY-21].
Citations
View all 12 citations: Citation Explorer
A Sensitive Near-Infrared Fluorescent Sensor for Mitochondrial Hydrogen Sulfide
Authors: Ji, A., Fan, Y., Ren, W., Zhang, S., Ai, H. W.
Journal: ACS Sens (2018): 992-997
Authors: Ji, A., Fan, Y., Ren, W., Zhang, S., Ai, H. W.
Journal: ACS Sens (2018): 992-997
Imaging intracellular quantum dots: fluorescence microscopy and transmission electron microscopy
Authors: Szymanski, C. J., Yi, H., Liu, J. L., Wright, E. R., Payne, C. K.
Journal: Methods Mol Biol (2013): 21-33
Authors: Szymanski, C. J., Yi, H., Liu, J. L., Wright, E. R., Payne, C. K.
Journal: Methods Mol Biol (2013): 21-33
Reversible off-on fluorescence probe for hypoxia and imaging of hypoxia-normoxia cycles in live cells
Authors: Takahashi, S., Piao, W., Matsumura, Y., Komatsu, T., Ueno, T., Terai, T., Kamachi, T., Kohno, M., Nagano, T., Hanaoka, K.
Journal: J Am Chem Soc (2012): 19588-91
Authors: Takahashi, S., Piao, W., Matsumura, Y., Komatsu, T., Ueno, T., Terai, T., Kamachi, T., Kohno, M., Nagano, T., Hanaoka, K.
Journal: J Am Chem Soc (2012): 19588-91
Family of enhanced photoacoustic imaging agents for high-sensitivity and multiplexing studies in living mice
Authors: de la Zerda, A., Bodapati, S., Teed, R., May, S. Y., Tabakman, S. M., Liu, Z., Khuri-Yakub, B. T., Chen, X., Dai, H., Gambhir, S. S.
Journal: ACS Nano (2012): 4694-701
Authors: de la Zerda, A., Bodapati, S., Teed, R., May, S. Y., Tabakman, S. M., Liu, Z., Khuri-Yakub, B. T., Chen, X., Dai, H., Gambhir, S. S.
Journal: ACS Nano (2012): 4694-701
Visualizing mechanical tension across membrane receptors with a fluorescent sensor
Authors: Stabley, D. R., Jurchenko, C., Marshall, S. S., Salaita, K. S.
Journal: Nat Methods (2011): 64-7
Authors: Stabley, D. R., Jurchenko, C., Marshall, S. S., Salaita, K. S.
Journal: Nat Methods (2011): 64-7
A comparative study of the binding of QSY 21 and Rhodamine 6G fluorescence probes to DNA: structure and dynamics
Authors: Kabelac, M., Zim and l, F., Fessl, T., Chval, Z., Lankas, F.
Journal: Phys Chem Chem Phys (2010): 9677-84
Authors: Kabelac, M., Zim and l, F., Fessl, T., Chval, Z., Lankas, F.
Journal: Phys Chem Chem Phys (2010): 9677-84
Pyrenebutyrate Leads to Cellular Binding, Not Intracellular Delivery, of Polyarginine-Quantum Dots
Authors: Jablonski, A. E., Kawakami, T., Ting, A. Y., Payne, C. K.
Journal: J Phys Chem Lett (2010): 1312-1315
Authors: Jablonski, A. E., Kawakami, T., Ting, A. Y., Payne, C. K.
Journal: J Phys Chem Lett (2010): 1312-1315
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin "quencher-chaser": a dual "quench and chase" strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: Ogawa, M., Kosaka, N., Choyke, P. L., Kobayashi, H.
Journal: Bioconjug Chem (2009): 147-54
Authors: Ogawa, M., Kosaka, N., Choyke, P. L., Kobayashi, H.
Journal: Bioconjug Chem (2009): 147-54
Dissolved core alginate microspheres as "smart-tattoo" glucose sensors
Authors: Chaudhary, A., Raina, M., McShane, M. J., Srivastava, R.
Journal: Conf Proc IEEE Eng Med Biol Soc (2009): 4098-101
Authors: Chaudhary, A., Raina, M., McShane, M. J., Srivastava, R.
Journal: Conf Proc IEEE Eng Med Biol Soc (2009): 4098-101
Evaluation of glucose sensitive affinity binding assay entrapped in fluorescent dissolved-core alginate microspheres
Authors: Chaudhary, A., Raina, M., Harma, H., Hanninen, P., McShane, M. J., Srivastava, R.
Journal: Biotechnol Bioeng (2009): 1075-85
Authors: Chaudhary, A., Raina, M., Harma, H., Hanninen, P., McShane, M. J., Srivastava, R.
Journal: Biotechnol Bioeng (2009): 1075-85
References
View all 1 references: Citation Explorer
Synthesis and characterization of a small, membrane-permeant, caspase-activatable far-red fluorescent peptide for imaging apoptosis
Authors: Bullok K, Piwnica-Worms D.
Journal: J Med Chem (2005): 5404
Authors: Bullok K, Piwnica-Worms D.
Journal: J Med Chem (2005): 5404