Rapamycin-LC-Fluorescein

Rapamycin-LC-Fluorescein is an excellent fluorescent probe to characterize FK506-binding proteins by fluorescence polarization. It has moderate affinity for the FK1 domains of FKBPS1 (177 nM) and FKBP2 (469 nM). It can be used for active-site titrations, competition assays with unlabeled ligands and enable a robust, miniaturized assay adequate for high-throughput screening. FK506-binding proteins (FKBPs) convey the immunosuppressive action of FK506 and rapamycin and mediate the neuroprotective properties of these compounds and participate in the regulation of calcium channels. In addition, the larger homologues FKBP51 and FKBP52 act as cochaperones for Hsp90 and regulate the transactivation activity of steroid hormone receptors.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
36706	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rapamycin-LC-Fluorescein to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	69.314 µL	346.572 µL	693.145 µL	3.466 mL	6.931 mL
5 mM	13.863 µL	69.314 µL	138.629 µL	693.145 µL	1.386 mL
10 mM	6.931 µL	34.657 µL	69.314 µL	346.572 µL	693.145 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1442.7 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

A pH-response-based fluorescent probe for detecting the mitophagy process by tracing changes in colocalization coefficients.

Authors:

Hao, Nongyi and Jiang, Zekun and Zhou, Lina and Dai, Xiaoyu and Kong, Xiuqi

Journal:

Analytical methods : advancing methods and applications (2024): 2241-2247

Verteporfin fluorescence in antineoplastic-treated pancreatic cancer cells found concentrated in mitochondria.

Authors:

Zhang, Ying-Qiao and Liu, Qing-Hao and Liu, Lu and Guo, Peng-Yu and Wang, Run-Ze and Ba, Zhi-Chang

Journal:

World journal of gastrointestinal oncology (2024): 968-978

A Systematic Design and Synthesis of PET-based Fluorescent Probes for Monitoring pH During Mitophagy.

Authors:

Yadav, Rashmi and Munan, Subrata and Ali, Mudassar and Mapa, Koyeli and Samanta, Animesh

Journal:

Chemistry, an Asian journal (2023): e202300308

Fluorescent probe for the identification of potent inhibitors of the macrophage infectivity potentiator (Mip) protein of Burkholderia pseudomallei.

Authors:

Scheuplein, Nicolas Julian and Lohr, Theresa and Vivoli Vega, Mirella and Ankrett, Dyan and Seufert, Florian and Kirchner, Lukas and Harmer, Nicholas J and Holzgrabe, Ulrike

Journal:

SLAS discovery : advancing life sciences R & D (2023): 211-222

A Reversible Fluorescent Probe for In Situ Monitoring Redox Imbalance during Mitophagy.

Authors:

Li, Junbin and Song, Lifei and Hu, Weiguo and Zuo, Qin and Li, Roumei and Dai, Min and Zhou, Yibo and Qing, Zhihe

Journal:

Analytical chemistry (2023): 13668-13673

Physical properties

Molecular weight	1442.70
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 26, 2025