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RatioWorks™ Cal-520®/zFluor 647™ -Dextran Conjugate
MW 10,000
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520® dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. RatioWorks™ Cal-520®/zFluor 647™-Dextran Conjugate carries a calcium-independent zFluor 647 fluorophore as a reference color for ratiometric measurements. The Cal-520® fluorophore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520® fluorophore has minimal excitation. This Cal-520® dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.
RatioWorks™ Cal-520®/zFluor 647™-Dextran Conjugate carries a calcium-independent zFluor 647 fluorphore as a reference color for ratiometric measurements. The Cal-520 fluorphore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520 fluorphore has minimal excitation. This Cal-520 dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.
RatioWorks™ Cal-520®/zFluor 647™-Dextran Conjugate carries a calcium-independent zFluor 647 fluorphore as a reference color for ratiometric measurements. The Cal-520 fluorphore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520 fluorphore has minimal excitation. This Cal-520 dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
206041 mg
Price
 
Physical properties
Molecular weight~12000
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 17, 2025