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RatioWorks™ Cal-520®/zFluor 647™ -Dextran Conjugate *MW 10,000*

RatioWorks™ Cal-520®/zFluor 647™-Dextran Conjugate carries a calcium-independent zFluor 647 fluorphore as a reference color for ratiometric measurements. The Cal-520 fluorphore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520 fluorphore has minimal excitation. This Cal-520 dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.
RatioWorks™ Cal-520®/zFluor 647™-Dextran Conjugate carries a calcium-independent zFluor 647 fluorphore as a reference color for ratiometric measurements. The Cal-520 fluorphore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520 fluorphore has minimal excitation. This Cal-520 dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.
Ordering information
Price ()
Catalog Number20604
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. RatioWorks™ Cal-520®/zFluor 647™-Dextran Conjugate carries a calcium-independent zFluor 647 fluorophore as a reference color for ratiometric measurements. The Cal-520 fluorophore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520 fluorophore has minimal excitation. This Cal-520 dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.

References


View all 30 references: Citation Explorer
In vivo measurements of cytosolic calcium in Dictyostelium discoideum.
Authors: Allan, Claire Y and Fisher, Paul R
Journal: Methods in molecular biology (Clifton, N.J.) (2009): 291-308
Organization of the olfactory pathway and odor processing in the antennal lobe of the ant Camponotus floridanus.
Authors: Zube, Christina and Kleineidam, Christoph Johannes and Kirschner, Sebastian and Neef, Jakob and Rössler, Wolfgang
Journal: The Journal of comparative neurology (2008): 425-41
Calcium regulation in individual peripheral sensory nerve terminals of the rat.
Authors: Gover, Tony D and Moreira, Thaís H V and Kao, Joseph P Y and Weinreich, Daniel
Journal: The Journal of physiology (2007): 481-90
Characterization of calcium oscillation patterns in caprine oocytes induced by IVF or an activation technique used in nuclear transfer.
Authors: Jellerette, Teru and Melican, David and Butler, Robin and Nims, Scott and Ziomek, Carol and Fissore, Rafael and Gavin, William
Journal: Theriogenology (2006): 1575-86
Involvement of TRP-like channels in the acute ischemic response of hippocampal CA1 neurons in brain slices.
Authors: Lipski, Janusz and Park, Thomas I H and Li, Dong and Lee, Stanley C W and Trevarton, Alexander J and Chung, Kenny K H and Freestone, Peter S and Bai, Ji-Zhong
Journal: Brain research (2006): 187-99
UV-A induces two calcium waves in Physcomitrella patens.
Authors: Tucker, Edward B and Lee, Michelle and Alli, Shaan and Sookhdeo, Vinoud and Wada, Masamitsu and Imaizumi, Takato and Kasahara, Masahiro and Hepler, Peter K
Journal: Plant & cell physiology (2005): 1226-36
Calcium gradients in conifer pollen tubes; dynamic properties differ from those seen in angiosperms.
Authors: Lazzaro, Mark D and Cardenas, Luis and Bhatt, Aadra P and Justus, Charles D and Phillips, Monique S and Holdaway-Clarke, Terena L and Hepler, Peter K
Journal: Journal of experimental botany (2005): 2619-28
Atrial natriuretic peptide attenuates Ca2+ oscillations and modulates plasma membrane Ca2+ fluxes in rat hepatocytes.
Authors: Green, Anne K and Zolle, Olga and Simpson, Alec W M
Journal: Gastroenterology (2002): 1291-303
Muscarinic signalling affects intracellular calcium concentration during the first cell cycle of sea urchin embryos.
Authors: Harrison, P K and Falugi, C and Angelini, C and Whitaker, M J
Journal: Cell calcium (2002): 289-97
Spontaneous calcium oscillatory patterns in mammotropes display non-random dynamics.
Authors: Shorte, S L and Faught, W J and Frawley, L S
Journal: Cell calcium (2000): 171-9