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RatioWorks™ Cal-520®/zFluor 647™ -Dextran Conjugate *MW 10,000*

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Cells may be physically loaded with the cell-impermeant dextran-conjugated calcium indicators using patch pipette or microinjection. The fluorescence signal from these cells is measured using fluorescence microscopy. The dextran forms of our calcium indicators show a dramatic reduction in both leakage and compartmentalization compared to the AM ester forms. Among the fluorescent calcium indicator dextran conjugates, Cal-520 dextran conjugates might be the best choice due to their high fluorescence quantum yield and large fluorescence enhancement by calcium. RatioWorks™ Cal-520®/zFluor 647™-Dextran Conjugate carries a calcium-independent zFluor 647 fluorophore as a reference color for ratiometric measurements. The Cal-520 fluorophore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520 fluorophore has minimal excitation. This Cal-520 dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.

References

View all 30 references: Citation Explorer
In vivo measurements of cytosolic calcium in Dictyostelium discoideum.
Authors: Allan, Claire Y and Fisher, Paul R
Journal: Methods in molecular biology (Clifton, N.J.) (2009): 291-308
Organization of the olfactory pathway and odor processing in the antennal lobe of the ant Camponotus floridanus.
Authors: Zube, Christina and Kleineidam, Christoph Johannes and Kirschner, Sebastian and Neef, Jakob and Rössler, Wolfgang
Journal: The Journal of comparative neurology (2008): 425-41
Calcium regulation in individual peripheral sensory nerve terminals of the rat.
Authors: Gover, Tony D and Moreira, Thaís H V and Kao, Joseph P Y and Weinreich, Daniel
Journal: The Journal of physiology (2007): 481-90
Characterization of calcium oscillation patterns in caprine oocytes induced by IVF or an activation technique used in nuclear transfer.
Authors: Jellerette, Teru and Melican, David and Butler, Robin and Nims, Scott and Ziomek, Carol and Fissore, Rafael and Gavin, William
Journal: Theriogenology (2006): 1575-86
Involvement of TRP-like channels in the acute ischemic response of hippocampal CA1 neurons in brain slices.
Authors: Lipski, Janusz and Park, Thomas I H and Li, Dong and Lee, Stanley C W and Trevarton, Alexander J and Chung, Kenny K H and Freestone, Peter S and Bai, Ji-Zhong
Journal: Brain research (2006): 187-99
Page updated on November 3, 2024

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Catalog Number20604
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Physical properties

Molecular weight

~12000

Solvent

Water

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
RatioWorks&trade; Cal-520&reg;/zFluor 647&trade;-Dextran Conjugate carries a calcium-independent zFluor 647 fluorphore as a reference color for ratiometric measurements. The Cal-520 fluorphore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520 fluorphore has minimal excitation. This Cal-520 dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.
RatioWorks&trade; Cal-520&reg;/zFluor 647&trade;-Dextran Conjugate carries a calcium-independent zFluor 647 fluorphore as a reference color for ratiometric measurements. The Cal-520 fluorphore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520 fluorphore has minimal excitation. This Cal-520 dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.
RatioWorks&trade; Cal-520&reg;/zFluor 647&trade;-Dextran Conjugate carries a calcium-independent zFluor 647 fluorphore as a reference color for ratiometric measurements. The Cal-520 fluorphore can be well excited by Argon laser at 488 nm where zFluor 647 has minimal excitation. The reference zFluor 647 fluorophore can be well excited by He-Ne laser at 633 nm or red laser at 647 nm where Cal-520 fluorphore has minimal excitation. This Cal-520 dextran exhibits high affinity to calcium ion than Cal-520L dextran (#20603), and optimized for monitoring low level calcium ion.