Name: RatioWorks™ PDMPO, SE
Brand: AAT Bioquest
SKU: 21210
Price: 232 USD
Availability: InStock

RatioWorks™ PDMPO, SE

The existing pH probes are ill-adapted to study acidic organelles such as lysosomes, endosomes, phagosomes, spermatozoa and acrosomes because their fluorescence is significantly reduced at lower pH. The growing potential of ratio imaging is significantly limited by the lack of appropriate fluorescent probes for acidic organelles although ratio imaging has received intensive attention in the past few decades. RatioWorks™ PDMPO is characterized as acidotropic dual-excitation and dual-emission pH probe. It emits intense yellow fluorescence at lower pH and gives intense blue fluorescence at higher pH. This unique pH-dependent fluorescence makes RatioWorks™ PDMPO an ideal pH probe for acidic organelles with pKa = 4.47. Additionally, the very large Stokes shift and excellent photostability of RatioWorks™ PDMPO make it an excellent fluorescent acidotropic reagent for fluorescence imaing and flow cytometry applications. The unique fluorescence properties of RatioWorks™ PDMPO might give researchers a new tool with which to study endocytosis, phagocytosis and acidic organelles of live cells. RatioWorks™ PDMPO can be well excited by the violet laser at 405 nm for flow cytometric applications. This RatioWorks™ PDMPO SE can be readily used to make a variety of bioconjugates for imaging or flow applications, enabling the specific detection of phagocytosis and endocytosis with reduced signal variability and improved accuracy. These conjugates can be also used for multiplexing cell functional analysis with green dyes such as GFP, Fluo-8, calcein, or FITC-labeled antibodies. The short emission band of RatioWorks™ PDMPO is ~450 nm while the longer emission is ~550 nm, making the common filter sets of Pacific Blue and Pacific Orange readily available to the assays of RatioWorks™ PDMPO.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21210	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of RatioWorks™ PDMPO, SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	197.426 µL	987.128 µL	1.974 mL	9.871 mL	19.743 mL
5 mM	39.485 µL	197.426 µL	394.851 µL	1.974 mL	3.949 mL
10 mM	19.743 µL	98.713 µL	197.426 µL	987.128 µL	1.974 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	506.52 g/mol	=	mL	x	mM	=	mmol

References

View all 56 references: Citation Explorer

Monitoring phospholipid dynamics during phagocytosis: application of genetically-encoded fluorescent probes

Authors:

Sarantis H, Grinstein S.

Journal:

Methods Cell Biol (2012): 429

Phagocytosis and digestion of pH-sensitive fluorescent dye (Eos-FP) transfected E. coli in whole blood assays from patients with severe sepsis and septic shock

Authors:

Schreiner L, Huber-Lang M, Weiss ME, Hohmann H, Schmolz M, Schneider EM.

Journal:

J Cell Commun Signal (2011): 135

The application of fluorescent probes for the analysis of lipid dynamics during phagocytosis

Authors:

Flannagan RS, Grinstein S.

Journal:

Methods Mol Biol (2010): 121

Quantification of microsized fluorescent particles phagocytosis to a better knowledge of toxicity mechanisms

Authors:

Leclerc L, Boudard D, Pourchez J, Forest V, Sabido O, Bin V, Palle S, Grosseau P, Bernache D, Cottier M.

Journal:

Inhal Toxicol (2010): 1091

Analysis of macrophage phagocytosis: quantitative assays of phagosome formation and maturation using high-throughput fluorescence microscopy

Authors:

Steinberg BE, Grinstein S.

Journal:

Methods Mol Biol (2009): 45

Physical properties

Molecular weight	506.52
Solvent	DMSO

Spectral properties

Excitation (nm)	333
Emission (nm)	531

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025