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AAT Bioquest

ReadiCleave™ Cy5-SSL-NHS ester

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ Cy5-SSL-NHS ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the Cy5 fluorophore from the target molecule. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
ReadiCleave™ AML Cy5 NHS ester65167025000010.271, 0.420.03
ReadiCleave™ XFD532 SSL-NHS ester534553810000.6110.09
ReadiCleave™ FAM SSL-NHS ester491516730000.920.35

References

View all 50 references: Citation Explorer
Repurposing an atherosclerosis targeting peptide for tumor imaging.
Authors: Kovacs, Luciana and Davis, Ryan A and Ganguly, Tanushree and Chammas, Roger and Sutcliffe, Julie L
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2022): 112469
Small molecule based EGFR targeting of biodegradable nanoparticles containing temozolomide and Cy5 dye for greatly enhanced image-guided glioblastoma therapy.
Authors: Schmitt, Rebecca R and Mahajan, Supriya D and Pliss, Artem and Prasad, Paras N
Journal: Nanomedicine : nanotechnology, biology, and medicine (2022): 102513
Nucleic Acid-Gated Covalent Organic Frameworks for Cancer-Specific Imaging and Drug Release.
Authors: Gao, Peng and Shen, Xiaoying and Liu, Xiaohan and Chen, Yuanyuan and Pan, Wei and Li, Na and Tang, Bo
Journal: Analytical chemistry (2021): 11751-11757
Near-Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette.
Authors: Abe, Masahiro and Nishihara, Ryo and Kim, Sung-Bae and Suzuki, Koji
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 103-110
On-resin strategy to label α-conotoxins: Cy5-RgIA, a potent α9α10 nicotinic acetylcholine receptor imaging probe.
Authors: Muttenthaler, Markus and Nevin, Simon T and Inserra, Marco and Lewis, Richard J and Adams, David J and Alewood, Paul
Journal: Australian journal of chemistry (2020): 327-333
Page updated on December 6, 2024

Ordering information

Price
Unit size
Catalog Number7050
Quantity
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Additional ordering information

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Physical properties

Molecular weight

1018.33

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.271, 0.42

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure