ReadiCleave™ FAM SSL-NHS ester

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	636.65
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

636.65

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.92

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ FAM SSL-NHS ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the fluorescein (FITC) fluorophore from the target molecule. 5-FAM has the same fluorophore identical to the common FITC dye. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ FAM SSL-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	157.072 µL	785.361 µL	1.571 mL	7.854 mL	15.707 mL
5 mM	31.414 µL	157.072 µL	314.144 µL	1.571 mL	3.141 mL
10 mM	15.707 µL	78.536 µL	157.072 µL	785.361 µL	1.571 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
ReadiCleave™ Cy5-SSL-NHS ester	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
ReadiCleave™ XFD532 SSL-NHS ester	534	553	81000	0.61¹	0.24	0.09

Images

Figure 1. ReadiCleave™ FAM SSL-NHS ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the fluorescein (FITC) fluorophore from the target molecule. 5-FAM has the same fluorophore identical to the common FITC dye. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.

References

View all 28 references: Citation Explorer

Fluorescein CorneoGraphy (FCG): Use of a repurposed fluorescein imaging technique to objectively standardize corneal staining.
Authors: Soifer, Matias and Azar, Nadim S and Blanco, Ricardo and Mousa, Hazem M and Ghalibafan, Seyyedehfatemeh and Tovar, Arianna and Mettu, Priyatham S and Allingham, Michael J and Cousins, Scott W and Sabater, Alfonso L and Perez, Victor L
Journal: The ocular surface (2023): 77-79

Image-Based Longitudinal Characterization of Corneal Wound to Understand the Role of Sphingosine-1-Phosphate.
Authors: Basu, Sandip K and Mandal, Nawajes
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 337-345

The TGM2 inhibitor cysteamine hydrochloride does not impact corneal epithelial and stromal wound healing in vitro and in vivo.
Authors: Minella, A L and Casanova, M I and Chokshi, T J and Kang, J and Cosert, K and Gragg, M M and Bowman, M A and Mccorkell, M E and Daley, N L and Leonard, B C and Murphy, C J and Raghunathan, V K and Thomasy, S M
Journal: Experimental eye research (2023): 109338

Focused ultrasound stimulation on meibomian glands for the treatment of evaporative dry eye.
Authors: Lu, Gengxi and Gollapudi, Sumanth and Li, Runze and Pfeiffer, Margaret L and Mehta, Preeya and Jiang, Laiming and Hamm-Alvarez, Sarah and Humayun, Mark and Zhou, Qifa and Zhang-Nunes, Sandy X
Journal: Experimental biology and medicine (Maywood, N.J.) (2022): 519-526

Human platelet lysate delivered via an ocular wound chamber for the treatment of corneal epithelial injuries.
Authors: Griffith, Gina L and Holt, Andrew W and Eriksson, Elof and Johnson, Anthony J and McDaniel, Jennifer S
Journal: Experimental eye research (2021): 108493

Synthesis, Characterization, and Specific Localization of Mitochondrial-Targeted Antioxidant Peptide SS31 Probe.
Authors: He, Yuan and Zhang, Ruixue and Quan, Zhuoya and He, Beilei and Xu, Yun and Chen, Zejun and Ren, Yuan and Liu, Xu
Journal: BioMed research international (2021): 9915699

Peripheral retinal leakage in POEMS syndrome.
Authors: Carey, Andrew Rising and Jeyaseelan, Praveen
Journal: International journal of retina and vitreous (2021): 6

Retinal capillary degeneration and blood-retinal barrier disruption in murine models of Alzheimer's disease.
Authors: Shi, Haoshen and Koronyo, Yosef and Fuchs, Dieu-Trang and Sheyn, Julia and Wawrowsky, Kolja and Lahiri, Shouri and Black, Keith L and Koronyo-Hamaoui, Maya
Journal: Acta neuropathologica communications (2020): 202

Effect of rutin on retinal VEGF, TNF-α, aldose reductase, and total antioxidant capacity in diabetic rats: molecular mechanism and ocular pharmacokinetics.
Authors: Gupta, Suresh Kumar and Sharma, Hanuman Prasad and Das, Ujjalkumar and Velpandian, Thirumurthy and Saklani, Ravi
Journal: International ophthalmology (2020): 159-168

Fluorescence Video Angiography for Evaluation of Dynamic Perfusion Status in an Aneurysm Preclinical Experimental Setting.
Authors: Grüter, Basil Erwin and Täschler, Dominik and Rey, Jeannine and Strange, Fabio and Nevzati, Edin and Fandino, Javier and Marbacher, Serge and Coluccia, Daniel
Journal: Operative neurosurgery (Hagerstown, Md.) (2019): 432-438