ReadiCleave™ iFluor 488 SSL-NHS ester
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1064.14 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | SDSProtocol |
Molecular weight 1064.14 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ iFluor™ 488 SSL-NHS ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the iFluor™ 488 fluorophore from the target molecule. iFluor™ 488 has almost identical spectra of Alexa Fluor 488 with high fluorescence quantum yield and improved stability compared the traditional FITC dye. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ iFluor 488 SSL-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 93.973 µL | 469.863 µL | 939.726 µL | 4.699 mL | 9.397 mL |
5 mM | 18.795 µL | 93.973 µL | 187.945 µL | 939.726 µL | 1.879 mL |
10 mM | 9.397 µL | 46.986 µL | 93.973 µL | 469.863 µL | 939.726 µL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ iFluor 488 AML-NHS ester | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
Images
References
View all 50 references: Citation Explorer
Release Mechanisms of Amorphous Solid Dispersions: Role of Drug-Polymer Phase Separation and Morphology.
Authors: Yang, Ruochen and Zhang, Geoff G Z and Zemlyanov, Dmitry Y and Purohit, Hitesh S and Taylor, Lynne S
Journal: Journal of pharmaceutical sciences (2023): 304-317
Authors: Yang, Ruochen and Zhang, Geoff G Z and Zemlyanov, Dmitry Y and Purohit, Hitesh S and Taylor, Lynne S
Journal: Journal of pharmaceutical sciences (2023): 304-317
Staining of stratum corneum with fluorescent ε-poly-L-lysine and its application to evaluation of skin conditions.
Authors: Yoshida, Moemi and Numajiri, Sana and Notani, Nao and Sato, Nao and Nomoto, Koji and Arikawa, Hitomi and Urabe, Hiroya and Ichikawa, Hideyuki and Akimoto, Ryuji and Sato, Jun-Ichi and Yamashita, Yuji and Hirao, Tetsuji
Journal: Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] Internatio (2023): e13245
Authors: Yoshida, Moemi and Numajiri, Sana and Notani, Nao and Sato, Nao and Nomoto, Koji and Arikawa, Hitomi and Urabe, Hiroya and Ichikawa, Hideyuki and Akimoto, Ryuji and Sato, Jun-Ichi and Yamashita, Yuji and Hirao, Tetsuji
Journal: Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] Internatio (2023): e13245
Agmatine production by Escherichia coli cells expressing SpeA on the extracellular surface.
Authors: Suzuki, Hideyuki and Thongbhubate, Kullathida and Muraoka, Madoka and Sasabu, Asuka
Journal: Enzyme and microbial technology (2023): 110139
Authors: Suzuki, Hideyuki and Thongbhubate, Kullathida and Muraoka, Madoka and Sasabu, Asuka
Journal: Enzyme and microbial technology (2023): 110139
Exclusion of Anchor-Matched Peptide Nucleic Acid from Liquid-Ordered Domains by Hybridization with Complementary Flavin-Labeled DNA.
Authors: Oka, Yoshimi
Journal: ACS omega (2023): 1109-1113
Authors: Oka, Yoshimi
Journal: ACS omega (2023): 1109-1113
Antitumor activity of bovine lactoferrin and its derived peptides against HepG2 liver cancer cells and Jurkat leukemia cells.
Authors: Arredondo-Beltrán, Izamar G and Ramírez-Sánchez, Diana A and Zazueta-García, Jesús R and Canizalez-Roman, Adrián and Angulo-Zamudio, Uriel A and Velazquez-Roman, Jorge A and Bolscher, Jan G M and Nazmi, Kamran and León-Sicairos, Nidia
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2023)
Authors: Arredondo-Beltrán, Izamar G and Ramírez-Sánchez, Diana A and Zazueta-García, Jesús R and Canizalez-Roman, Adrián and Angulo-Zamudio, Uriel A and Velazquez-Roman, Jorge A and Bolscher, Jan G M and Nazmi, Kamran and León-Sicairos, Nidia
Journal: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine (2023)
Retinal Input to the Primate Lateral Geniculate Nucleus Revealed by Injection of a Different Label Into Each Eye.
Authors: Spahr, Zachary R and Economides, John R and Horton, Jonathan C
Journal: Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society (2022): e596-e597
Authors: Spahr, Zachary R and Economides, John R and Horton, Jonathan C
Journal: Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society (2022): e596-e597
[Effect of electroacupuncture on ocular surface sensory neuralgia and expression of P2X3 receptor and PKC in cornea and trigeminal ganglion of dry eye guinea pigs].
Authors: Shen, Hu-Xing and Zheng, Xiao-Jun and Jin, Tuo and Li, Pei-Chen and Gao, Wei-Ping and Wei, Qing-Bo and Sun, Xin-Yi and Xie, Ling
Journal: Zhen ci yan jiu = Acupuncture research (2022): 393-400
Authors: Shen, Hu-Xing and Zheng, Xiao-Jun and Jin, Tuo and Li, Pei-Chen and Gao, Wei-Ping and Wei, Qing-Bo and Sun, Xin-Yi and Xie, Ling
Journal: Zhen ci yan jiu = Acupuncture research (2022): 393-400
Independent actions by HIV-1 Tat and morphine to increase recruitment of monocyte-derived macrophages into the brain in a region-specific manner.
Authors: Leibrand, Crystal R and Paris, Jason J and Jones, Austin M and Ohene-Nyako, Michael and Rademeyer, Kara M and Nass, Sara R and Kim, Woong-Ki and Knapp, Pamela E and Hauser, Kurt F and McRae, MaryPeace
Journal: Neuroscience letters (2022): 136852
Authors: Leibrand, Crystal R and Paris, Jason J and Jones, Austin M and Ohene-Nyako, Michael and Rademeyer, Kara M and Nass, Sara R and Kim, Woong-Ki and Knapp, Pamela E and Hauser, Kurt F and McRae, MaryPeace
Journal: Neuroscience letters (2022): 136852
Detection of Homologous Blood Transfusion in Sport Doping by Flow Cytofluorimetry: State of the Art and New Approaches to Reduce the Risk of False-Negative Results.
Authors: Donati, Francesco and de la Torre, Xavier and Pagliarosi, Sarajane and Pirri, Daniela and Prevete, Giuliana and Botrè, Francesco
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Authors: Donati, Francesco and de la Torre, Xavier and Pagliarosi, Sarajane and Pirri, Daniela and Prevete, Giuliana and Botrè, Francesco
Journal: Frontiers in sports and active living (2022): 808449
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Journal: Physiological reports (2022): e15334
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Journal: Physiological reports (2022): e15334