Power Styramide™ Signal Amplification (PSA) system is one of the most sensitive fluorescence imaging methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the commonly used tyramide (TSA) reagents. ReadiCleave™ Styramides are the new iteration of our PSA™ products that add the reversible capability to chemically remove the PSA™ staining on tissue or in cells if needed. As our other PSA™ reagents, the ReadiCleave™ Styramides are an excellent collection of multicolor reagents to simultaneously detect multiple targets in the same tissue samples. They provide an additional benefit, i.e., the PSA™ staining can be removed (if desired) while preserving the integrity of tissue samples. A specific protein is first recognized by its selective primary antibody. Subsequently, the target is stained by the HRP-secondary antibody conjugate of its immunoglobulin class. The bright red, fluorescent ReadiCleave ™ iFluor® 546 Styramide is added subsequently. The HRP-antibody conjugate catalyzes the coupling reaction between ReadiCleave™ iFluor® 546 Styramide and the target protein in proximity. The red iFluor® 546 Styramide staining can be gently removed with nearly 100% efficiency using ReadiCleave™ AML Cleavage Buffer when needed.