Name: ReadiCleave™ iFluor 594 AML-NHS ester
Brand: AAT Bioquest
SKU: 7007
Price: 430 USD
Availability: InStock

ReadiCleave™ iFluor 594 AML-NHS ester

Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, almost all the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ iFluor® 594 AML uses an azidomethyl linker that can be cleaved with TCEP to remove the iFluor® 594 fluorophore from the target molecule. The cleavage can be carried out by adding 10-100 mM TCEP solution (pH 7.5) and incubating at 65 °C for 1-5 min. iFluor® 594 is a superior replacement to Alexa Fluor® 594. iFluor® 594 and Alexa Fluor® 594 have very similar spectral properties.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
7007	1 mg	Price

References

View all 50 references: Citation Explorer

MicroRNA-126 inhibits pathological retinal neovascularization via suppressing vascular endothelial growth factor expression in a rat model of retinopathy of prematurity.

Authors:

Fan, Yuan-Yao and Liu, Chi-Hsien and Wu, An-Lun and Chen, Hung-Chi and Hsueh, Yi-Jen and Chen, Kuan-Jen and Lai, Chi-Chun and Huang, Chung-Ying and Wu, Wei-Chi

Journal:

European journal of pharmacology (2021): 174035

Retinal ganglion cells projecting to superior colliculus and pulvinar in marmoset.

Authors:

Grünert, Ulrike and Lee, Sammy C S and Kwan, William C and Mundinano, Inaki-Carril and Bourne, James A and Martin, Paul R

Journal:

Brain structure & function (2021)

A fully integrated isotachophoresis with a programmable microfluidic platform.

Authors:

Shebindu, Adam and Somaweera, Himali and Estlack, Zachary and Kim, Jungtae and Kim, Jungkyu

Journal:

Talanta (2021): 122039

Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides.

Authors:

Iwata, Takahiro and Hirose, Hisaaki and Sakamoto, Kentarou and Hirai, Yusuke and Arafiles, Jan Vincent V and Akishiba, Misao and Imanishi, Miki and Futaki, Shiroh

Journal:

Angewandte Chemie (International ed. in English) (2021)

Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).

Authors:

Seo, Hyesu and Kim, Kyunghee and Park, Woong June

Journal:

Biomolecules (2021)

Physical properties

Molecular weight	1509.77
Solvent	DMSO

Spectral properties

Absorbance (nm)	587
Correction factor (260 nm)	0.05
Correction factor (280 nm)	0.04
Extinction coefficient (cm ^-1 M ^-1)	200000 ¹
Excitation (nm)	587
Emission (nm)	603
Quantum yield	0.53 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 18, 2025