Power Styramide™ Signal Amplification (PSA) system is one of the most sensitive fluorescence imaging methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the commonly used tyramide (TSA) reagents. ReadiCleave™ Styramides are the new iteration of our PSA products that add the reversible capability to chemically remove the PSA staining on tissue or in cells if needed. As our other PSA reagents, the ReadiCleave™ Styramides are an excellent collection of multicolor reagents to simultaneously detect multiple targets in the same tissue samples. They provide an additional benefit, i.e., the PSA staining can be removed (if desired) while preserving the integrity of tissue samples. A specific protein is first recognized by its selective primary antibody. Subsequently, the target is stained by the HRP-secondary antibody conjugate of its immunoglobulin class. The bright deep red, fluorescent ReadiCleave ™ iFluor® 647 Styramide is added subsequently. The HRP-antibody conjugate catalyzes the coupling reaction between ReadiCleave™ iFluor® 647 Styramide and the target protein in proximity. The deep red fluorescence imaging can be easily recorded with the Cy5 filter set. The deep red iFluor® 647 Styramide staining can be gently removed with nearly 100% efficiency using ReadiCleave™ AML Cleavage Buffer when needed.