ReadiCleave™ iFluor 700 SSL-NHS ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1140.41 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | SDSProtocol |
Molecular weight 1140.41 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis or another detection mode. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. ReadiCleave™ iFluor® 700 succinimidyl ester contains a S-S linker that can be cleaved with TCEP or DTT to remove the iFluor® 700 fluorophore from the target molecule. iFluor® 700 has almost identical spectra of Alexa Fluor 700 with higher fluorescence quantum yield and improved stability. The cleavage can be carried out by adding DTT or TCEP solution and incubating from room temperature to 65 °C for 1-10 min.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ iFluor 700 SSL-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 87.688 µL | 438.439 µL | 876.878 µL | 4.384 mL | 8.769 mL |
5 mM | 17.538 µL | 87.688 µL | 175.376 µL | 876.878 µL | 1.754 mL |
10 mM | 8.769 µL | 43.844 µL | 87.688 µL | 438.439 µL | 876.878 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (565 nm) | Correction Factor (650 nm) |
ReadiCleave™ iFluor 700 AML-NHS ester | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 | 0.0766 | 0.4004 |
ReadiCleave™ iFluor 488 SSL-NHS ester | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 | - | - |
References
View all 30 references: Citation Explorer
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Journal: ACS nano (2021): 5077-5085
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