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AAT Bioquest

ReadiCleave™ XFD488 AML-NHS ester

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML XFD488 contains an azidomethyl linker that can be cleaved with TCEP to remove the Alexa Fluor® 488 fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. XFD488 is the same fluorophore to Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ReadiCleave™ AML Cy5 NHS ester65167025000010.271, 0.420.020.03
ReadiCleave™ XFD532 AML-NHS ester534553810000.6110.240.09
ReadiCleave™ FITC AML-NHS ester4985178000010.79001, 0.9520.320.35
ReadiCleave™ XFD546 AML-NHS ester5615721120000.7910.210.12
ReadiCleave™ XFD647 AML-NHS ester6506712390000.3310.000.03

References

View all 50 references: Citation Explorer
Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Detection of Homologous Blood Transfusion in Sport Doping by Flow Cytofluorimetry: State of the Art and New Approaches to Reduce the Risk of False-Negative Results.
Authors: Donati, Francesco and de la Torre, Xavier and Pagliarosi, Sarajane and Pirri, Daniela and Prevete, Giuliana and Botrè, Francesco
Journal: Frontiers in sports and active living (2022): 808449
A fluorescent aptasensor for the detection of Aflatoxin B1 by graphene oxide mediated quenching and release of fluorescence.
Authors: Setlem, Sai Keerthana and Mondal, Bhairab and Ramlal, Shylaja
Journal: Journal of microbiological methods (2022): 106414
Introducing Cysteines into Nanobodies for Site-Specific Labeling.
Authors: Hansen, Simon Boje and Andersen, Kasper Røjkjær
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 327-343
Dynamics of intracellular neonatal Fc receptor-ligand interactions in primary macrophages using biophysical fluorescence techniques.
Authors: Pannek, Andreas and Houghton, Fiona J and Verhagen, Anne M and Dower, Steven K and Hinde, Elizabeth and Gleeson, Paul A
Journal: Molecular biology of the cell (2022): ar6
Page updated on November 12, 2024

Ordering information

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Catalog Number7008
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Physical properties

Molecular weight

1340.49

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.3

Correction Factor (280 nm)

0.11

Extinction coefficient (cm -1 M -1)

73000

Excitation (nm)

499

Emission (nm)

520

Quantum yield

0.921

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure