ReadiCleave™ XFD488 AML-NHS ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1340.49 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.3 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 499 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Direct upgrades
ReadiCleave™ iFluor 488 AML-NHS ester |
Related products
Overview | SDSProtocol |
Molecular weight 1340.49 | Correction Factor (260 nm) 0.3 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 73000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.921 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML XFD488 contains an azidomethyl linker that can be cleaved with TCEP to remove the Alexa Fluor® 488 fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. XFD488 is the same fluorophore to Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ XFD488 AML-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 74.6 µL | 372.998 µL | 745.996 µL | 3.73 mL | 7.46 mL |
5 mM | 14.92 µL | 74.6 µL | 149.199 µL | 745.996 µL | 1.492 mL |
10 mM | 7.46 µL | 37.3 µL | 74.6 µL | 372.998 µL | 745.996 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.3 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 73000 |
Excitation (nm) | 499 |
Emission (nm) | 520 |
Quantum yield | 0.921 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ AML Cy5 NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
ReadiCleave™ XFD532 AML-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
ReadiCleave™ FITC AML-NHS ester | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
ReadiCleave™ XFD546 AML-NHS ester | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
ReadiCleave™ XFD647 AML-NHS ester | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
References
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Detection of Homologous Blood Transfusion in Sport Doping by Flow Cytofluorimetry: State of the Art and New Approaches to Reduce the Risk of False-Negative Results.
Authors: Donati, Francesco and de la Torre, Xavier and Pagliarosi, Sarajane and Pirri, Daniela and Prevete, Giuliana and Botrè, Francesco
Journal: Frontiers in sports and active living (2022): 808449
Authors: Donati, Francesco and de la Torre, Xavier and Pagliarosi, Sarajane and Pirri, Daniela and Prevete, Giuliana and Botrè, Francesco
Journal: Frontiers in sports and active living (2022): 808449
Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
A fluorescent aptasensor for the detection of Aflatoxin B1 by graphene oxide mediated quenching and release of fluorescence.
Authors: Setlem, Sai Keerthana and Mondal, Bhairab and Ramlal, Shylaja
Journal: Journal of microbiological methods (2022): 106414
Authors: Setlem, Sai Keerthana and Mondal, Bhairab and Ramlal, Shylaja
Journal: Journal of microbiological methods (2022): 106414
Introducing Cysteines into Nanobodies for Site-Specific Labeling.
Authors: Hansen, Simon Boje and Andersen, Kasper Røjkjær
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 327-343
Authors: Hansen, Simon Boje and Andersen, Kasper Røjkjær
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 327-343
Dynamics of intracellular neonatal Fc receptor-ligand interactions in primary macrophages using biophysical fluorescence techniques.
Authors: Pannek, Andreas and Houghton, Fiona J and Verhagen, Anne M and Dower, Steven K and Hinde, Elizabeth and Gleeson, Paul A
Journal: Molecular biology of the cell (2022): ar6
Authors: Pannek, Andreas and Houghton, Fiona J and Verhagen, Anne M and Dower, Steven K and Hinde, Elizabeth and Gleeson, Paul A
Journal: Molecular biology of the cell (2022): ar6
Gold nanoparticles enhance fluorescence signals by flow cytometry at low antibody concentrations.
Authors: Reis, Daniela S and de Oliveira, Vivian L and Silva, Misael L and Paniago, Roberto M and Ladeira, Luiz O and Andrade, Lidia M
Journal: Journal of materials chemistry. B (2021): 1414-1423
Authors: Reis, Daniela S and de Oliveira, Vivian L and Silva, Misael L and Paniago, Roberto M and Ladeira, Luiz O and Andrade, Lidia M
Journal: Journal of materials chemistry. B (2021): 1414-1423
Binding of cytochrome P450 27C1, a retinoid desaturase, to its accessory protein adrenodoxin.
Authors: Glass, Sarah M and Webb, Stephany N and Guengerich, F Peter
Journal: Archives of biochemistry and biophysics (2021): 109076
Authors: Glass, Sarah M and Webb, Stephany N and Guengerich, F Peter
Journal: Archives of biochemistry and biophysics (2021): 109076
Pegylated-Catalase Is Protective in Lung Ischemic Injury and Oxidative Stress.
Authors: Kim, Jung-Lye and Reader, Brenda F and Dumond, Curtis and Lee, Yonggyu and Mokadam, Nahush A and Black, Sylvester M and Whitson, Bryan A
Journal: The Annals of thoracic surgery (2021): 1019-1027
Authors: Kim, Jung-Lye and Reader, Brenda F and Dumond, Curtis and Lee, Yonggyu and Mokadam, Nahush A and Black, Sylvester M and Whitson, Bryan A
Journal: The Annals of thoracic surgery (2021): 1019-1027
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Journal: Brain structure & function (2021)
Authors: Grünert, Ulrike and Lee, Sammy C S and Kwan, William C and Mundinano, Inaki-Carril and Bourne, James A and Martin, Paul R
Journal: Brain structure & function (2021)
An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity.
Authors: Wu, Siwen and Wang, Lingling and Tang, Elizabeth I and Wang, Junlu and Cheng, C Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2021)
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Journal: Methods in molecular biology (Clifton, N.J.) (2021)