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AAT Bioquest

ReadiCleave™ XFD532 AML-NHS ester

Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML XFD532 contains an azidomethyl linker that can be cleaved with TCEP to remove the XFD532 fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. XFD532 is the same fluorophore to Alexa Fluor® 532 (Alexa Fluor® is the trademark of ThermoFisher).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ReadiCleave™ AML Cy5 NHS ester65167025000010.271, 0.420.020.03
ReadiCleave™ FITC AML-NHS ester4985178000010.79001, 0.9520.320.35
ReadiCleave™ XFD488 AML-NHS ester499520730000.9210.30.11
ReadiCleave™ XFD546 AML-NHS ester5615721120000.7910.210.12
ReadiCleave™ XFD532 SSL-NHS ester534553810000.6110.240.09
ReadiCleave™ XFD647 AML-NHS ester6506712390000.3310.000.03

References

View all 17 references: Citation Explorer
Encapsulation of Plant Viral Particles in Calcite Crystals.
Authors: Al-Handawi, Marieh B and Commins, Patrick and Shukla, Sourabh and Didier, Pascal and Tanaka, Masahiko and Raj, Gijo and Veliz, Frank A and Pasricha, Renu and Steinmetz, Nicole F and Naumov, Panče
Journal: Advanced biosystems (2018): e1700176
Multilayer Hydrogel Capsules of Interpenetrated Network for Encapsulation of Small Molecules.
Authors: Kozlovskaya, Veronika and Chen, Jun and Zavgorodnya, Oleksandra and Hasan, Mohammad B and Kharlampieva, Eugenia
Journal: Langmuir : the ACS journal of surfaces and colloids (2018): 11832-11842
Light emitting diode, photodiode-based fluorescence detection system for DNA analysis with microchip electrophoresis.
Authors: Hall, Gordon H and Glerum, D Moira and Backhouse, Christopher J
Journal: Electrophoresis (2016): 406-13
Assessing the efficacy of co-inoculation of wheat seedlings with the associative bacteria Paenibacillus polymyxa 1465 and Azospirillum brasilense Sp245.
Authors: Yegorenkova, Irina V and Tregubova, Kristina V and Burygin, Gennady L and Matora, Larisa Y and Ignatov, Vladimir V
Journal: Canadian journal of microbiology (2016): 279-85
Engineered drug-protein nanoparticle complexes for folate receptor targeting.
Authors: Ren, Dongmei and Kratz, Felix and Wang, Szu-Wen
Journal: Biochemical engineering journal (2014): 33-41
Page updated on December 6, 2024

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Catalog Number7001
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Physical properties

Molecular weight

1174.31

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.09

Extinction coefficient (cm -1 M -1)

81000

Excitation (nm)

534

Emission (nm)

553

Quantum yield

0.611

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501