ReadiCleave™ XFD532 AML-NHS ester
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML XFD532 contains an azidomethyl linker that can be cleaved with TCEP to remove the XFD532 fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. XFD532 is the same fluorophore to Alexa Fluor® 532 (Alexa Fluor® is the trademark of ThermoFisher).
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ AML Cy5 NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
ReadiCleave™ FITC AML-NHS ester | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
ReadiCleave™ XFD488 AML-NHS ester | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
ReadiCleave™ XFD546 AML-NHS ester | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
ReadiCleave™ XFD532 SSL-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
ReadiCleave™ XFD647 AML-NHS ester | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
References
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Journal: Biochemical engineering journal (2014): 33-41
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