ReadiCleave™ XFD532 AML-NHS ester
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1174.31 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.24 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 81000 |
Excitation (nm) | 534 |
Emission (nm) | 553 |
Quantum yield | 0.611 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
Molecular weight 1174.31 | Correction Factor (260 nm) 0.24 | Correction Factor (280 nm) 0.09 | Extinction coefficient (cm -1 M -1) 81000 | Excitation (nm) 534 | Emission (nm) 553 | Quantum yield 0.611 |
Fluorescence-based methods have many advantages for biological detections in terms of sensitivity and convenience. Many biological molecules can be readily labeled with a fluorescent tag for fluorescence imaging and flow cytometry analysis. However, most of the existing fluorescent tags are used to permanently labeling biological targets from which the added fluorescent tags cannot be cleaved for further downstream analysis, such as mass spectral analysis. AAT Bioquest’s ReadiCleave™ linkers enable fluorescent tags conjugated to a biological target from which the added fluorescent tag can be removed when needed. This ReadiCleave™ AML XFD532 contains an azidomethyl linker that can be cleaved with TCEP to remove the XFD532 fluorophore from the target molecule. The cleavage can be carried out by adding 10 mM TCEP solution (pH 7.5), and incubating at 65 °C for 1-5 min. XFD532 is the same fluorophore to Alexa Fluor® 532 (Alexa Fluor® is the trademark of ThermoFisher).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ReadiCleave™ XFD532 AML-NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 85.156 µL | 425.782 µL | 851.564 µL | 4.258 mL | 8.516 mL |
5 mM | 17.031 µL | 85.156 µL | 170.313 µL | 851.564 µL | 1.703 mL |
10 mM | 8.516 µL | 42.578 µL | 85.156 µL | 425.782 µL | 851.564 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.24 |
Correction Factor (280 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 81000 |
Excitation (nm) | 534 |
Emission (nm) | 553 |
Quantum yield | 0.611 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
ReadiCleave™ AML Cy5 NHS ester | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
ReadiCleave™ FITC AML-NHS ester | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
ReadiCleave™ XFD488 AML-NHS ester | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
ReadiCleave™ XFD546 AML-NHS ester | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
ReadiCleave™ XFD532 SSL-NHS ester | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
ReadiCleave™ XFD647 AML-NHS ester | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
References
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