ReadiLink™ Cy5 Oligo and ssDNA Labeling Kit

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Direct upgrades

ReadiLink™ iFluor® 647 Oligo and ssDNA Labeling Kit

Overview SDS Protocol

Upgrade: ReadiLink™ iFluor® 647 Oligo and ssDNA Labeling Kit

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm ^-1 M ^-1)

250000¹

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.27¹, 0.4²

ReadiLink™ Cy5 Oligo and ssDNA Labelling Kit enables simple and uniform tagging of single-stranded DNA or oligos with Cy5 fluorophore. The labelling kit uses our proprietary TAQuest™ terminal deoxynucleotidyl transferase (TdT) to catalyze non-template directed nucleotide incorporation onto the 3’- end of single-stranded DNAs or oligos. The kit is optimized for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos. The resulting Cy5-labelled DNA probes are ideally suited for biological applications, e.g., electrophoretic mobility shift assays (EMSA), Northern and Southern blots, colony or in situ hybridizations.

Platform

Thermal Cycler

Instrument specification(s)

0.5 mL microcentrifuge or 0.2 mL PCR tube

Components

Example protocol

AT A GLANCE

Protocol summary

Prepare oligo or ssDNA samples
Add reagents to tube
Mix and centrifuge briefly
Incubate at 37 °C for 60 minutes
Place on ice for 5 minutes
Purify the labeled DNA

Note: Thaw all the kit components on ice before starting the experiment. Briefly centrifuge all the reagents to the bottom before starting the labeling process.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline.

To a clean (Nuclease-free) 0.5 mL microcentrifuge tube or 0.2 mL PCR tube, prepare a reaction mix by adding the reagents in the order indicated in Table 1.
Carefully mix the reagents by a brief vortex, followed by a brief centrifuge.
Incubate the reaction at 37 °C for 60 minutes.
After incubation, place the reaction on ice for 5 minutes.
Purify the labeled DNA.

Table 1.Reagents composition per tube for each reaction

Components	Amount
Oligo or ssDNA sample	1 µg DNA diluted in Nuclease-free water to a final volume of 5 µL
TdT Reaction Buffer	40 µL
Cy5-dUTP	1-2 µL
CoCl₂	5 µL
TdT enzyme	0.5 µL
Total Volume	52 µL (Approx.)

Note: The amount of Cy5-dUTP can be optimized to achieve the best labeling conditions.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
ReadiLink™ Cy3 Oligo and ssDNA Labeling Kit	555	569	150000¹	0.15¹	0.07	0.073

References

View all 2 references: Citation Explorer

A High-Throughput Image Correlation Method for Rapid Analysis of Fluorophore Photoblinking and Photobleaching Rates.
Authors: Sehayek, Simon and Gidi, Yasser and Glembockyte, Viktorija and Brandão, Hugo B and François, Paul and Cosa, Gonzalo and Wiseman, Paul W
Journal: ACS nano (2019): 11955-11966

Base-Excision-Repair-Induced Construction of a Single Quantum-Dot-Based Sensor for Sensitive Detection of DNA Glycosylase Activity.
Authors: Wang, Li-Juan and Ma, Fei and Tang, Bo and Zhang, Chun-Yang
Journal: Analytical chemistry (2016): 7523-9