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ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit
Fluorescence in situ hybridization (FISH) technology is an effective tool for detecting specific nucleic acid targets in a biological specimen. Detection of a nucleic acid target in situ is achieved through the hybridization of a fluorescent dye-labeled nucleic acid probe of complementary sequence to the specimen. The Readilink™ iFluor® 488 FISH fluorescence imaging kit is a convenient tool for labeling a target DNA using an iFluor® 488 labeled FISH probe via in situ hybridization. The kit provides Taq DNA polymerase enzyme, which incorporates iFluor® 488-dUTPs in the target DNA through Polymerase Chain Reaction (PCR). Our proprietary iFluor® dyes are brighter and more photostable than traditional fluorescent labels, providing the desired resolution and signal.
Telomere quantitative fluorescence in situ hybridization in metaphase HeLa cells using iFluor® 488-dUTP labeled telomere probes. Probes were created using the ReadiLink™ iFluor® 488 FISH Fluorescence Imaging kit.
Telomere quantitative fluorescence in situ hybridization in metaphase HeLa cells using iFluor® 488-dUTP labeled telomere probes. Probes were created using the ReadiLink™ iFluor® 488 FISH Fluorescence Imaging kit.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
1731025 reactions
Price
 
Physical properties
SolventWater
Spectral properties
Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Instrument settings
Thermal Cycler
Recommended platePCR Microplate
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Page updated on September 27, 2025