logo
AAT Bioquest

ReadiLink™ iFluor® 647 FISH Fluorescence Imaging Kit

Telomere quantitative fluorescence in situ hybridization in metaphase HeLa cells using iFluor® 647-dUTP labeled telomere probes. Probes were created using the ReadiLink™ iFluor® 647 FISH Fluorescence Imaging kit.
Telomere quantitative fluorescence in situ hybridization in metaphase HeLa cells using iFluor® 647-dUTP labeled telomere probes. Probes were created using the ReadiLink™ iFluor® 647 FISH Fluorescence Imaging kit.
Telomere quantitative fluorescence in situ hybridization in metaphase HeLa cells using iFluor® 647-dUTP labeled telomere probes. Probes were created using the ReadiLink™ iFluor® 647 FISH Fluorescence Imaging kit.
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
SolventWater
Spectral properties
Correction Factor (260 nm)0.03
Correction Factor (280 nm)0.03
Correction Factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)656
Emission (nm)670
Quantum yield0.251
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Related products
ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Yellow 630 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Red 780 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 350 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 594 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*
ReadiLink™ Rapid trFluor™ Tb Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*
ReadiLink™ Protein Conjugation Stop Buffer
ReadiLink™ BSA Conjugation Kit
ReadiLink™ KLH Conjugation Kit
ReadiLink™ Protein Biotinylation Kit
ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology*
ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ xtra Rapid iFluor® 350 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 488 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 555 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 594 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 647 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 750 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid FITC Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid Cy3 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid Cy5 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid Cy7 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid XFD594 Antibody Labeling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594*
ReadiLink™ Rapid XFD488 Antibody Labeling Kit *XFD488 Same Structure to Alexa Fluor™ 488*
ReadiLink™ Rapid XFD555 Antibody Labeling Kit *XFD555 Same Structure to Alexa Fluor™ 555*
ReadiLink™ Rapid XFD594 Antibody Labeling Kit *XFD594 Same Structure to Alexa Fluor™ 594*
ReadiLink™ Rapid XFD647 Antibody Labeling Kit *XFD647 Same Structure to Alexa Fluor™ 647*
ReadiLink™ Rapid XFD750 Antibody Labeling Kit *XFD750 Same Structure to Alexa Fluor™ 750*
ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit
ReadiLink™ iFluor® 555 Nick Translation dsDNA Labeling Kit
ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit
ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit
ReadiLink™ Cy5 Nick Translation dsDNA Labeling Kit
ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit
ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit
ReadiLink™ iFluor® 488 Oligo and ssDNA Labeling Kit
ReadiLink™ iFluor® 555 Oligo and ssDNA Labeling Kit
ReadiLink™ iFluor® 647 Oligo and ssDNA Labeling Kit
ReadiLink™ Cy3 Oligo and ssDNA Labeling Kit
ReadiLink™ Cy5 Oligo and ssDNA Labeling Kit
ReadiLink™ Biotin Oligo and ssDNA Labeling Kit
ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit
ReadiLink™ xtra Rapid XFD488 Antibody Labeling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488*
ReadiLink™ xtra Rapid XFD555 Antibody Labeling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555*
ReadiLink™ xtra Rapid XFD647 Antibody Labeling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647*
ReadiLink™ xtra Rapid XFD750 Antibody Labeling Kit *BSA-Compatible, XFD750 Same Structure to Alexa Fluor™ 750*
ReadiLink™ Rapid iFluor® 350 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid iFluor® 488 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid iFluor® 555 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid iFluor® 594 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid iFluor® 750 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid FITC Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid XFD488 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid XFD555 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid XFD594 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid XFD647 Antibody Labeling Kit *Production Scale*
ReadiLink™ Rapid XFD750 Antibody Labeling Kit *Production Scale*
ReadiLink™ Psoralen-A17-Biotin
ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
Show More (71)

OverviewpdfSDSpdfProtocol


Correction Factor (260 nm)
0.03
Correction Factor (280 nm)
0.03
Correction Factor (656 nm)
0.0793
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
656
Emission (nm)
670
Quantum yield
0.251
Fluorescence in situ hybridization (FISH) technology is an effective tool for detecting specific nucleic acid targets in a biological specimen. Detection of a nucleic acid target in situ is achieved through the hybridization of a fluorescent dye-labeled nucleic acid probe of complementary sequence to the specimen. The Readilink™ iFluor® 647 FISH fluorescence imaging kit is a convenient tool for labeling a target DNA using an iFluor® 647 labeled FISH probe via in situ hybridization. The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 647-dUTPs in the target DNA through Polymerase Chain Reaction (PCR). Our proprietary iFluor® dyes are brighter and more photostable than traditional fluorescent labels, providing the desired resolution and signal.

Platform


Thermal Cycler

Recommended platePCR Microplate

Components


Example protocol


SAMPLE EXPERIMENTAL PROTOCOL

Before using, thaw all components to room temperature and mix thoroughly by vortexing.

Note: The following protocol can be used as a general guideline to standard DNA FISH. Optimization may be necessary for your experimental system.

  1. Prepare the following reaction mixes as indicated in Table 1.

    Table 1. Reagents composition per well for each reaction.

    ComponentsVolume (25 µL/reaction)Final Conc.
    FISH Reaction mix (2X)12.5 µL1X
    Upstream primer, 10 µM0.25-2.5 µL0.1-1.0 µM
    Downstream primer, 10 µM0.25-2.5 µL0.1-1.0 µM
    DNA template1-5 µLOptimized conc.
    iFluor® 647-dUTP2.5 µL 
    dNTP mix1 µL 
    Water, nuclease-free25 µL 
  2. Carefully mix the reagents by gentle vortexing followed by a brief centrifuge.

  3. Set up the plate in the qPCR instrument and run as indicated in Table 2.

    Table 2. Thermal cycling parameters.

    Parameter

    Polymerase Activation

    PCR (30-40 cycles)

     

    Hold

    Denature

    Anneal

    Extend

    Temperature

    95 °C

    95 °C

    55-65 °C

    68-72 °C

    Time (m:ss)

    0:20

    0:30

    1:00

    1:00

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.03
Correction Factor (280 nm)0.03
Correction Factor (656 nm)0.0793
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)656
Emission (nm)670
Quantum yield0.251

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit4915167500010.910.210.11
ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit55757010000010.6410.230.14

Images


References


View all 32 references: Citation Explorer
Quantitative intracellular retention of delivered RNAs through optimized cell fixation and immunostaining.
Authors: Paramasivam, Prasath and Stöter, Martin and Corradi, Eloina and Dalla Costa, Irene and Höijer, Andreas and Bartesaghi, Stefano and Sabirsh, Alan and Lindfors, Lennart and Yanez Arteta, Marianna and Nordberg, Peter and Andersson, Shalini and Baudet, Marie-Laure and Bickle, Marc and Zerial, Marino
Journal: RNA (New York, N.Y.) (2022): 433-446
In Situ Super-Resolution Imaging of Telomeres with DNA-PAINT.
Authors: Liu, Yuanyuan and Ye, Xiangyu and Wang, Zhuyuan and Zong, Shenfei and Cui, Yiping
Journal: ACS omega (2022): 40512-40519
A Janus 3D DNA nanomachine for simultaneous and sensitive fluorescence detection and imaging of dual microRNAs in cancer cells.
Authors: Yang, Zezhou and Peng, Xin and Yang, Peng and Zhuo, Ying and Chai, Ya-Qin and Liang, Wenbin and Yuan, Ruo
Journal: Chemical science (2020): 8482-8488
Dual-probe RNA FRET-FISH in Yeast.
Authors: Wadsworth, Gable M and Parikh, Rasesh Y and Kim, Harold D
Journal: Bio-protocol (2018): e2867
A molecular pathology method for sequential fluorescence in situ hybridization for multi-gene analysis at the single-cell level.
Authors: Hu, Linping and Yin, Xiuxiu and Sun, Jiangman and Zetterberg, Anders and Miao, Weimin and Cheng, Tao
Journal: Oncotarget (2017): 50534-50541
mRNA detection in budding yeast with single fluorophores.
Authors: Wadsworth, Gable M and Parikh, Rasesh Y and Choy, John S and Kim, Harold D
Journal: Nucleic acids research (2017): e141
Chondroitin sulfate-polyethylenimine copolymer-coated superparamagnetic iron oxide nanoparticles as an efficient magneto-gene carrier for microRNA-encoding plasmid DNA delivery.
Authors: Lo, Yu-Lun and Chou, Han-Lin and Liao, Zi-Xian and Huang, Shih-Jer and Ke, Jyun-Han and Liu, Yu-Sheng and Chiu, Chien-Chih and Wang, Li-Fang
Journal: Nanoscale (2015): 8554-65
Quantitation and localization of pospiviroids in aphids.
Authors: Van Bogaert, N and De Jonghe, K and Van Damme, E J M and Maes, M and Smagghe, G
Journal: Journal of virological methods (2015): 51-4
CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells.
Authors: Deng, Wulan and Shi, Xinghua and Tjian, Robert and Lionnet, Timothée and Singer, Robert H
Journal: Proceedings of the National Academy of Sciences of the United States of America (2015): 11870-5
Comparison of the sequence-dependent fluorescence of the cyanine dyes Cy3, Cy5, DyLight DY547 and DyLight DY647 on single-stranded DNA.
Authors: Kretschy, Nicole and Somoza, Mark M
Journal: PloS one (2014): e85605