ReadiLink™ iFluor® 647 Oligo and ssDNA Labeling Kit

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12171501

Overview SDS Protocol

Correction Factor (260 nm)

0.03

Correction Factor (280 nm)

0.03

Correction Factor (656 nm)

0.0793

Extinction coefficient (cm ^-1 M ^-1)

250000¹

Excitation (nm)

656

Emission (nm)

670

Quantum yield

0.25¹

ReadiLink™ iFluor® 647 Oligo and ssDNA Labelling Kit enables simple and uniform tagging of single-stranded DNA or oligos with iFluor® 647, our bright, photostable and green-fluorescent fluorophore. The labelling kit uses our proprietary TAQuest™ terminal deoxynucleotidyl transferase (TdT) to catalyze non-template directed nucleotide incorporation onto the 3’- end of single-stranded DNAs or oligos. The kit is optimized for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos. The resulting iFluor® 647-labelled DNA probes are ideally suited for biological applications, e.g., electrophoretic mobility shift assays (EMSA), Northern and Southern blots, colony or in situ hybridizations.

Platform

Thermal Cycler

Instrument specification(s)

0.5 mL microcentrifuge or 0.2 mL PCR tube

Components

Example protocol

AT A GLANCE

Protocol summary

Prepare oligo or ssDNA samples
Add reagents to tube
Mix and centrifuge briefly
Incubate at 37 °C for 60 minutes
Place on ice for 5 minutes
Purify the labeled DNA

Note: Thaw all the kit components on ice before starting the experiment. Briefly centrifuge all the reagents to the bottom before starting the labeling process.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline.

To a clean (Nuclease-free) 0.5 mL microcentrifuge tube or 0.2 mL PCR tube, prepare a reaction mix by adding the reagents in the order indicated in Table 1.
Carefully mix the reagents by a brief vortex, followed by a brief centrifuge.
Incubate the reaction at 37 °C for 60 minutes.
After incubation, place the reaction on ice for 5 minutes.
Purify the labeled DNA.

Table 1.Reagents composition per tube for each reaction

Components	Amount
Oligo or ssDNA sample	1 µg DNA diluted in Nuclease-free water to a final volume of 5 µL
TdT Reaction Buffer	40 µL
iFluor™ 647-dUTP	1-2 µL
CoCl₂	5 µL
TdT enzyme	0.5 µL
Total Volume	52 µL (Approx.)

Note: The amount of iFluor™ 647-dUTP can be optimized to achieve the best labeling conditions.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.03
Correction Factor (280 nm)	0.03
Correction Factor (656 nm)	0.0793
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	656
Emission (nm)	670
Quantum yield	0.25¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
ReadiLink™ iFluor® 488 Oligo and ssDNA Labeling Kit	491	516	75000¹	0.9¹	0.21	0.11
ReadiLink™ iFluor® 555 Oligo and ssDNA Labeling Kit	557	570	100000¹	0.64¹	0.23	0.14

References

View all 13 references: Citation Explorer

Chorus2: design of genome-scale oligonucleotide-based probes for fluorescence in situ hybridization.
Authors: Zhang, Tao and Liu, Guanqing and Zhao, Hainan and Braz, Guilherme T and Jiang, Jiming
Journal: Plant biotechnology journal (2021): 1967-1978

A Ratiometric Fluorescent Conjugated Oligomer for Amyloid β Recognition, Aggregation Inhibition, and Detoxification.
Authors: Zhang, Ziqi and Yuan, Qiong and Li, Meiqi and Bao, Benkai and Tang, Yanli
Journal: Small (Weinheim an der Bergstrasse, Germany) (2021): e2104581

General Method for Determining Redox Potentials without Electrolyte.
Authors: Bird, Matthew J and Pearson, Matthew A and Asaoka, Sadayuki and Miller, John R
Journal: The journal of physical chemistry. A (2020): 5487-5495

Breaking Parallel Orientation of Rods via a Dendritic Architecture toward Diverse Supramolecular Structures.
Authors: Zhang, Ruimeng and Feng, Xueyan and Zhang, Rui and Shan, Wenpeng and Su, Zebin and Mao, Jialin and Wesdemiotis, Chrys and Huang, Jiahao and Yan, Xiao-Yun and Liu, Tong and Li, Tao and Huang, Mingjun and Lin, Zhiwei and Shi, An-Chang and Cheng, Stephen Z D
Journal: Angewandte Chemie (International ed. in English) (2019): 11879-11885

Manufacture of Clinical-Grade Human Clonal Mesenchymal Stem Cell Products from Single Colony Forming Unit-Derived Colonies Based on the Subfractionation Culturing Method.
Authors: Yi, TacGhee and Kim, Si-na and Lee, Hyun-Joo and Kim, Junghee and Cho, Yun-Kyoung and Shin, Dong-Hee and Tak, Sun-Ji and Moon, Sun-Hwa and Kang, Ji-Eun and Ji, In-Mi and Lim, Huyn-Ja and Lee, Dong-Soon and Jeon, Myung-Shin and Song, Sun U
Journal: Tissue engineering. Part C, Methods (2015): 1251-62

Poly(styrene)/oligo(fluorene)-intercalated fluoromica hybrids: synthesis, characterization and self-assembly.
Authors: Leone, Giuseppe and Galeotti, Francesco and Porzio, William and Scavia, Guido and Barba, Luisa and Arrighetti, Gianmichele and Ricci, Giovanni and Botta, Chiara and Giovanella, Umberto
Journal: Beilstein journal of nanotechnology (2014): 2450-8

Synthesis of star-shaped monodisperse oligo(9,9-di-n-octylfluorene-2,7-vinylene)s functionalized truxenes with two-photon absorption properties.
Authors: Zhou, Huipeng and Zhao, Xin and Huang, Tianhao and Lu, Ran and Zhang, Hanzhuang and Qi, Xiaohui and Xue, Pengchong and Liu, Xingliang and Zhang, Xiaofei
Journal: Organic & biomolecular chemistry (2011): 1600-7

Monodisperse co-oligomer approach toward nanostructured films with alternating donor-acceptor lamellae.
Authors: Bu, Laju and Guo, Xiaoyang and Yu, Bo and Qu, Yao and Xie, Zhiyuan and Yan, Donghang and Geng, Yanhou and Wang, Fosong
Journal: Journal of the American Chemical Society (2009): 13242-3

Adsorption properties of oligo(fluorooxetane)-b-poly(ethylene oxide)-b-oligo(fluorooxetane) triblock copolymers at the air-water interface: comparison of hydroxyl and acetate end groups.
Authors: Ertekin, Ayça and Kim, Yongsin and Kausch, Charles M and Thomas, Richard R
Journal: Journal of colloid and interface science (2009): 40-5

Synthesis, characterization, adsorption, and interfacial rheological properties of four-arm anionic fluorosurfactants.
Authors: Ertekin, Ayça and Kausch, Charles M and Kim, Yongsin and Thomas, Richard R
Journal: Langmuir : the ACS journal of surfaces and colloids (2008): 2412-20