logo
AAT Bioquest

ReadiLink™ Protein Conjugation Stop Buffer

Mechanism for bioconjugation of dye to a peptide using succinimidyl ester.
Mechanism for bioconjugation of dye to a peptide using succinimidyl ester.
Mechanism for bioconjugation of dye to a peptide using succinimidyl ester.
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
QuotationRequest
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C)
UNSPSC12352200
Related products
ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Violet 510 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Green 620 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Yellow 630 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Red 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid mFluor™ Red 780 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 350 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 555 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 594 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 680 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 700 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 750 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 488 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 633 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid iFluor® 790 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid FITC Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*
ReadiLink™ Rapid trFluor™ Tb Antibody Labeling Kit *Microscale Optimized for Labeling 50 ug Antibody Per Reaction*
ReadiLink™ BSA Conjugation Kit
ReadiLink™ KLH Conjugation Kit
ReadiLink™ Protein Biotinylation Kit
ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology*
ReadiLink™ Rapid Cy3 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid Cy5 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ Rapid Cy7 Antibody Labeling Kit *Microscale Optimized for Labeling 50 μg Antibody Per Reaction*
ReadiLink™ xtra Rapid iFluor® 350 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 488 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 555 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 594 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 647 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid iFluor® 750 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid FITC Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid Cy3 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid Cy5 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid Cy7 Antibody Labeling Kit *BSA-Compatible*
ReadiLink™ xtra Rapid XFD594 Antibody Labeling Kit *BSA-Compatible, XFD594 Same Structure to Alexa Fluor™ 594*
ReadiLink™ Rapid XFD488 Antibody Labeling Kit *XFD488 Same Structure to Alexa Fluor™ 488*
ReadiLink™ Rapid XFD555 Antibody Labeling Kit *XFD555 Same Structure to Alexa Fluor™ 555*
ReadiLink™ Rapid XFD594 Antibody Labeling Kit *XFD594 Same Structure to Alexa Fluor™ 594*
ReadiLink™ Rapid XFD647 Antibody Labeling Kit *XFD647 Same Structure to Alexa Fluor™ 647*
ReadiLink™ Rapid XFD750 Antibody Labeling Kit *XFD750 Same Structure to Alexa Fluor™ 750*
ReadiLink™ iFluor® 488 Nick Translation dsDNA Labeling Kit
ReadiLink™ iFluor® 555 Nick Translation dsDNA Labeling Kit
ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit
ReadiLink™ Cy3 Nick Translation dsDNA Labeling Kit
ReadiLink™ Cy5 Nick Translation dsDNA Labeling Kit
ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit
ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit
ReadiLink™ iFluor® 488 Oligo and ssDNA Labeling Kit
ReadiLink™ iFluor® 555 Oligo and ssDNA Labeling Kit
ReadiLink™ iFluor® 647 Oligo and ssDNA Labeling Kit
ReadiLink™ Cy3 Oligo and ssDNA Labeling Kit
ReadiLink™ Cy5 Oligo and ssDNA Labeling Kit
ReadiLink™ Biotin Oligo and ssDNA Labeling Kit
ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit
ReadiLink™ xtra Rapid XFD488 Antibody Labeling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488*
ReadiLink™ xtra Rapid XFD555 Antibody Labeling Kit *BSA-Compatible, XFD555 Same Structure to Alexa Fluor™ 555*
ReadiLink™ xtra Rapid XFD647 Antibody Labeling Kit *BSA-Compatible, XFD647 Same Structure to Alexa Fluor™ 647*
ReadiLink™ xtra Rapid XFD750 Antibody Labeling Kit *BSA-Compatible, XFD750 Same Structure to Alexa Fluor™ 750*
ReadiLink™ iFluor® 488 FISH Fluorescence Imaging Kit
ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit
ReadiLink™ iFluor® 647 FISH Fluorescence Imaging Kit
Show More (56)

OverviewpdfSDSpdfProtocol


ReadiLink™ Protein Conjugation Stop Buffer is used to stop the protein conjugation reactions that use succinimidyl ester, sulfonyl chloride or isothiocyanate. It can be used directly without dilution.

Example protocol


AT A GLANCE

Important      Warm up ReadiLink™ protein conjugation stop buffer at room temperature before use.

SAMPLE EXPERIMENTAL PROTOCOL

  1. Prepare your protein (antibody) conjugation reaction as needed.
  2. Stop Conjugation reaction by adding ReadiLink™ protein conjugation stop buffer at 1:50 ratio (such as for a 100 µL conjugation reaction, add 2 µL ReadiLink™ protein conjugation stop buffer).
  3. Incubate at room temperature for 10 minutes.
  4. The labeled protein (antibody) is now ready to use.
    Note     It is not necessary to purify conjugated proteins (antibodies) with a column after the conjugation reaction is stopped with ReadiLink™ protein conjugation stop buffer if the conjugation is used within 1 month.
    Note     For long term storage, it is recommended purifying the conjugated proteins (antibodies) with desalting column. And the protein conjugate should be stored at > 0.5 mg/mL in the presence of a carrier protein (e.g., 0.1% bovine serum albumin). For longer storage, the protein conjugates could be lyophilized or divided into single-used aliquots and stored at ≤ –20°C. 

Images