ReadiLink™ Protein Conjugation Stop Buffer
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Application notes
ReadiLink™ Protein Conjugation Stop Buffer is used to stop the protein conjugation reactions that use succinimidyl ester, sulfonyl chloride or isothiocyanate. It can be used directly without dilution.
![Mechanism for bioconjugation of dye to a peptide using succinimidyl ester.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Freadilink-protein-conjugation-stop-buffer%2Ffigure-for-readilink-protein-conjugation-stop-buffer_6Nxmh.jpg&w=640&q=75)
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![Mechanism for bioconjugation of dye to a peptide using succinimidyl ester.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Freadilink-protein-conjugation-stop-buffer%2Ffigure-for-readilink-protein-conjugation-stop-buffer_6Nxmh.jpg&w=1080&q=100)
Figure 1. Mechanism for bioconjugation of dye to a peptide using succinimidyl ester.
![Mechanism for bioconjugation of dye to a peptide using succinimidyl ester.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Freadilink-protein-conjugation-stop-buffer%2Ffigure-for-readilink-protein-conjugation-stop-buffer_6Nxmh.jpg&w=640&q=75)
![Mechanism for bioconjugation of dye to a peptide using succinimidyl ester.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Freadilink-protein-conjugation-stop-buffer%2Ffigure-for-readilink-protein-conjugation-stop-buffer_6Nxmh.jpg&w=128&q=25)
Ordering information
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Unit size | |
Catalog Number | 5400 |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
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Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C) |
UNSPSC | 12352200 |
Example protocol
AT A GLANCE
Important Warm up ReadiLink™ protein conjugation stop buffer at room temperature before use.
SAMPLE EXPERIMENTAL PROTOCOL
- Prepare your protein (antibody) conjugation reaction as needed.
- Stop Conjugation reaction by adding ReadiLink™ protein conjugation stop buffer at 1:50 ratio (such as for a 100 µL conjugation reaction, add 2 µL ReadiLink™ protein conjugation stop buffer).
- Incubate at room temperature for 10 minutes.
- The labeled protein (antibody) is now ready to use.
Note It is not necessary to purify conjugated proteins (antibodies) with a column after the conjugation reaction is stopped with ReadiLink™ protein conjugation stop buffer if the conjugation is used within 1 month.
Note For long term storage, it is recommended purifying the conjugated proteins (antibodies) with desalting column. And the protein conjugate should be stored at > 0.5 mg/mL in the presence of a carrier protein (e.g., 0.1% bovine serum albumin). For longer storage, the protein conjugates could be lyophilized or divided into single-used aliquots and stored at ≤ –20°C.
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ReadiLink™ Protein Conjugation Stop Buffer
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