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ReadiLink™ Rapid Cy3 Antibody Labeling Kit
Production Scale
ReadiLink™ Rapid Antibody Labeling Kits, designed for production scale, provide a convenient and efficient method for labeling large volumes of antibodies with our superior iFluor® dyes, XFD dyes (equivalent to Alexa Fluor®), and various other labels. These kits utilize reactive fluorophores modified with succinimidyl ester (SE) functional groups, which selectively bind to primary amines on proteins, resulting in remarkably bright and photostable conjugates. Every kit contains all the necessary components for three distinct labeling reactions and features a user-friendly, pre-packed spin column for efficient dye removal, maximizing conjugate yield. Each vial of Cy3 dye provided in the kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it is important to remove stabilizing proteins like BSA from the sample and refrain from using amine-rich buffers like Tris, which might disrupt the labeling process. The Cy3 dye is a bright, orange-fluorescent dye with excitation and emission maxima of ~555 nm and ~569 nm, respectively. Cy3 conjugates are ideal for imaging, flow cytometry, and genomic applications. With ReadiLink™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.
HeLa cells were labeled with mouse anti-tubulin followed by a goat anti-mouse IgG conjugated to Cy3 using the ReadiLink™ Rapid Cy3 Antibody Labeling Kit (Cat No. 5722).
HeLa cells were labeled with mouse anti-tubulin followed by a goat anti-mouse IgG conjugated to Cy3 using the ReadiLink™ Rapid Cy3 Antibody Labeling Kit (Cat No. 5722).
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
57223 x 1 mg
Price
 
Spectral properties
Correction factor (260 nm)0.07
Correction factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)
150000
1
Excitation (nm)555
Emission (nm)569
Quantum yield
0.15
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on February 14, 2026