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ReadiLink™ Rapid Cy5 Antibody Labeling Kit
Production Scale
ReadiLink™ Rapid Antibody Labeling Kits, designed for production scale, provide a convenient and efficient method for labeling large volumes of antibodies with our superior iFluor® dyes, XFD dyes (equivalent to Alexa Fluor®), and various other labels. These kits utilize reactive fluorophores modified with succinimidyl ester (SE) functional groups, which selectively bind to primary amines on proteins, resulting in remarkably bright and photostable conjugates. Every kit contains all the necessary components for three distinct labeling reactions and features a user-friendly, pre-packed spin column for efficient dye removal, maximizing conjugate yield. Each vial of Cy5 dye provided in the kit is precisely formulated to label 1 mg of purified protein or antibody. Before labeling, it is important to remove stabilizing proteins like BSA from the sample and refrain from using amine-rich buffers like Tris, which might disrupt the labeling process. The Cy5 dye is a bright red fluorescent dye with excitation and emission maxima of ~651 nm and ~670 nm, respectively. Long wavelength dyes like Cy5 have a benefit over other fluorophores due to the low autofluorescence of biological specimens within this region of the spectrum. Cy5 conjugates are ideal for imaging, flow cytometry, and genomic applications. With ReadiLink™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.
HeLa cells were labeled with mouse anti-tubulin followed by a goat anti-mouse IgG conjugated to Cy5 using the ReadiLink™ Rapid Cy5 Antibody Labeling Kit (Cat No. 5725).
HeLa cells were labeled with mouse anti-tubulin followed by a goat anti-mouse IgG conjugated to Cy5 using the ReadiLink™ Rapid Cy5 Antibody Labeling Kit (Cat No. 5725).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
57253 x 1 mg
Price
 
Spectral properties
Correction factor (260 nm)0.02
Correction factor (280 nm)0.03
Correction factor (482 nm)0.009
Correction factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)651
Emission (nm)670
Quantum yield
0.27
1
, 
0.4
2
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 23, 2025