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ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit
Microscale Optimized for Labeling 50 μg Antibody Per Reaction
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 450 dyes have fluorescence excitation and emission maxima of ~405 nm and ~450 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Blue™ labeling dye. mFluor™ Violet 450 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. This kit has all the essential components to perform two separate labeling reactions with no column purification needed. Each of the two vials of mFluor™ Violet 450 SE provided in the kit is optimized for labeling ~50 µg antibody. mFluor™ Violet 450 SE antibody labeling kit provides a convenient method to label small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Violet Laser-excitable mFluor™ Violet 450 SE.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with CD4 (RPA-T4) antibody labeled using ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit (Cat. #1100). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the V2-A channel, demonstrating clear detection of CD4⁺ lymphocytes.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of human whole blood stained with CD4 (RPA-T4) antibody labeled using ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit (Cat. #1100). The fluorescence signal was monitored on a Cytek Aurora spectral flow cytometer in the V2-A channel, demonstrating clear detection of CD4⁺ lymphocytes.
protocol
Protocol
sds
SDS
CatalogSize
Price
Quantity
11002 Labelings
Price
 
Spectral properties
Absorbance (nm)406
Correction factor (260 nm)0.338
Correction factor (280 nm)0.078
Extinction coefficient (cm -1 M -1)
35000
1
Excitation (nm)406
Emission (nm)445
Quantum yield
0.81
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on February 20, 2026