ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labeling Kits provide a quick and convenient method to label antibodies, proteins (>10 kDa), or other amine-containing biomolecules. Leveraging a unique conjugation chemistry, ReadiLink™ Antibody Labeling Kits enables researchers to label microscale volumes of antibodies in two easy steps, with no purification needed and 100% recovery of the labeled product. Each kit includes sufficient mFluor™ Violet 610 dye to facilitate two distinct labeling reactions of 50 µg antibody samples. The resulting conjugates are ideal for a wide range of applications, including flow cytometry, fluorescent microscopy techniques, immunocytochemistry (ICC), immunohistochemistry (IHC), ELISA, and indirect FISH. Compared to Alexa Fluor dyes and conventional dye counterparts, mFluor™ dyes are brighter, more photostable, and resistance to changes in pH between pH 4 to 10. The mFluor™ Violet 610 dyes are optimally excited by violet lasers and emit red fluorescence ~610 nm. These properties make them well-suited for spectral fluorescence flow cytometry, providing researchers with a tool for detailed biological analysis with unparalleled precision and sensitivity. With ReadiLink™ Rapid Antibody Labeling kits, researchers can directly label primary antibodies, eliminating the need for secondary antibodies and enhancing panel-building flexibility.

Figure 1. Overview of the ReadiLink™ Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.