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ReadiLink™ Rapid mFluor™ Yellow 630 Antibody Labeling Kit
Microscale Optimized for Labeling 50 μg Antibody Per Reaction
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers . mFluor™ Yellow 630 dyes have fluorescence excitation and emission maxima of ~561 nm and ~630 nm respectively, making its conjugates readily excitable by 561 nm yellow laser. These spectral characteristics make mFluor™ Yellow 630 an excellent choice to make yellow laser-excitable antibodies for flow cytometry applications. mFluor™ Yellow 630 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. This kit has all the essential components to perform two separate labeling reactions and purifications. Each of the two vials of mFluor™ Yellow 630 provided in the kit is optimized for labeling ~50 µg antibody. mFluor™ Yellow 630 antibody labeling kit provides a convenient method to label small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the mFluor™ Yellow 630 SE dye.
<strong>Readilink&trade; Kit Labeling Principle:&nbsp;</strong>1).<strong>&nbsp;<em><u>Start</u>&nbsp;</em></strong>the labeling reaction by mixing a labeling dye with a protein (to be labeled) in the Reaction Buffer (pH 7.5-8.5). 2).&nbsp;<strong><em><u>Incubation</u></em>&nbsp;</strong>gives a mixture of the desired protein conjugate and unreactive free dye. 3).&nbsp;<strong><em><u>Quench</u>&nbsp;</em></strong>the reaction by mixing a non-fluorescent Tide Quencher&trade; (TQ) dye with the reaction solution. The TQ dye stops the reaction AND converts the unreactive free labeling dye to the non-fluorescent TQ-Labeling dye complex, which eliminates the background fluorescence interference of the free labeling dye.
<strong>Readilink&trade; Kit Labeling Principle:&nbsp;</strong>1).<strong>&nbsp;<em><u>Start</u>&nbsp;</em></strong>the labeling reaction by mixing a labeling dye with a protein (to be labeled) in the Reaction Buffer (pH 7.5-8.5). 2).&nbsp;<strong><em><u>Incubation</u></em>&nbsp;</strong>gives a mixture of the desired protein conjugate and unreactive free dye. 3).&nbsp;<strong><em><u>Quench</u>&nbsp;</em></strong>the reaction by mixing a non-fluorescent Tide Quencher&trade; (TQ) dye with the reaction solution. The TQ dye stops the reaction AND converts the unreactive free labeling dye to the non-fluorescent TQ-Labeling dye complex, which eliminates the background fluorescence interference of the free labeling dye.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
11262 Labelings
Price
 
Spectral properties
Absorbance (nm)561
Extinction coefficient (cm -1 M -1)
110000
1
Excitation (nm)570
Emission (nm)632
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on July 26, 2023