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ReadiLink™ xtra Rapid FITC Antibody Labeling Kit
BSA-Compatible
ReadiLink™ xtra rapid antibody labeling kits require essentially only 2 simple mixing steps without a column purification needed. Specially formulated FITC used in this ReadiLink™ kit is quite stable and shows good reactivity and selectivity with antibodies. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of specially formulated FITC dye provided in the kit is optimized for labeling ~50 µg antibody. ReadiLink™ xtra FITC rapid antibody labeling kit provides a convenient and robust method to label monoclonal and polyclonal antibodies with the green fluorescent FITC fluorophore. FITC is one of the most commonly used fluorophores for labeling antibodies.

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ xtra Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Immunofluorescence staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with rabbit anti-tubulin monoclonal antibody and stained with a goat anti-rabbit IgG labeled using the ReadiLink™ xtra Rapid FITC Antibody Labeling Kit (Cat No. 1970).
Immunofluorescence staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with rabbit anti-tubulin monoclonal antibody and stained with a goat anti-rabbit IgG labeled using the ReadiLink™ xtra Rapid FITC Antibody Labeling Kit (Cat No. 1970).
CatalogSize
Price
Quantity
19702 Labelings
Price
 
Spectral properties

Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)491
Emission (nm)516
Quantum yield
0.92
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 30, 2025