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ReadiLink™ xtra Rapid iFluor® 488 Antibody Labeling Kit
BSA-Compatible
ReadiLink™ xtra rapid antibody labeling kits require essentially only 2 simple mixing steps without a column purification needed. Preactivated iFluor® 488 used in this ReadiLink™ kit is quite stable and shows good reactivity and selectivity with antibodies. The kit has all the essential components for labeling ~2x50 ug antibody. Each of the two vials of preactivated iFluor® 488 dye provided in the kit is optimized for labeling ~50 µg antibody. ReadiLink™ xtra iFluor® 488 rapid antibody labeling kit provides a convenient and robust method to label monoclonal and polyclonal antibodies with the bright green fluorescent iFluor® 488 fluorophore. AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm).

 

readilinkworkflow

 

Figure 1. Overview of the ReadiLink™ xtra Rapid Antibody Labeling protocol. In just two simple steps, and with no purification necessary, covalently label microgram amounts of antibodies in under an hour.

Immunofluorescence staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with mouse anti-tubulin antibody (1ug/ml) and stained with a goat anti-mouse IgG conjugate (5ug/ml) labeled using different methods:
<ol>
<li>GXM IgG-iFluor®488 (Cat#16448)</li>
<li>&nbsp;50ug GXM IgG in PBS with ReadiLink&trade; Rapid iFluor® 488 Antibody Labeling Kit (Cat#1255)</li>
<li>50ug GXM IgG in PBS + 0.05% BSA with ReadiLink&trade; Rapid iFluor® 488 Antibody Labeling Kit (Cat#1255)</li>
<li>50ug GXM IgG in PBS + 0.05% BSA with&nbsp; ReadiLink&trade; xtra Rapid iFluor® 488 Antibody Labeling Kit (Cat#1955)</li>
<li>50ug GXM IgG in PBS + 0.5% BSA with ReadiLink&trade; xtra Rapid iFluor® 488 Antibody Labeling Kit (Cat#1955)</li>
<li>GXM IgG-Alexa Fluor 488 (Vendor J)</li>
</ol>
Immunofluorescence&nbsp;staining of tubulin in HeLa cells. HeLa cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 and blocked. Cells were then incubated with mouse anti-tubulin antibody (1ug/ml) and stained with a goat anti-mouse IgG conjugate (5ug/ml) labeled using different&nbsp;methods:
<ol>
<li>GXM IgG-iFluor®488 (Cat#16448)</li>
<li>&nbsp;50ug GXM IgG in PBS with ReadiLink&trade; Rapid iFluor® 488 Antibody Labeling Kit (Cat#1255)</li>
<li>50ug GXM IgG in PBS + 0.05% BSA with ReadiLink&trade; Rapid iFluor® 488 Antibody Labeling Kit (Cat#1255)</li>
<li>50ug GXM IgG in PBS + 0.05% BSA with&nbsp; ReadiLink&trade; xtra Rapid iFluor® 488 Antibody Labeling Kit (Cat#1955)</li>
<li>50ug GXM IgG in PBS + 0.5% BSA with ReadiLink&trade; xtra Rapid iFluor® 488 Antibody Labeling Kit (Cat#1955)</li>
<li>GXM IgG-Alexa Fluor 488 (Vendor J)</li>
</ol>
CatalogSize
Price
Quantity
19552 Labelings
Price
 
Spectral properties

Correction factor (260 nm)0.21
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
75000
1
Excitation (nm)491
Emission (nm)516
Quantum yield
0.9
1
Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
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Page updated on October 8, 2025