Name: ReadiPrep™ CD4+ Cell Isolation Kit *Optimized for processing 30 mL blood*
Brand: AAT Bioquest
SKU: 67301
Price: 595 USD
Availability: InStock

ReadiPrep™ CD4+ Cell Isolation Kit

Optimized for processing 30 mL blood

ReadiPrep™ CD4+ Cell Isolation Kit delivers pure, bead-free CD4+ T cells with a rapid and reproducible workflow.

High efficiency: Selectively isolates CD4+ T cells from whole blood with high yield and purity
Bead-free recovery: Optimized release buffer ensures isolated cells are free of magnetic beads
Applications: Ideal for flow cytometry, gene expression profiling, immunological assays, and T cell functional studies
Comparable alternative: Provides a fast, scalable solution similar to Miltenyi Biotec’s CD4+ T Cell Isolation Kits with added bead-free recovery for improved downstream compatibility

CD4+ T cells positively isolated from whole blood using ReadiPrep™ CD4+ Cell Isolation Kit.
The figure shows that the percentage of CD4+ cells was 36.3% before isolation which increased to 99.38% after isolation. Cells were stained with CD4 (SK3)-iFluor® 488 and CD45 (2D1)-iFlour® 647.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
67301	for 30 mL blood	Price

Overview

The ReadiPrep™ CD4+ Cell Isolation Kit provides a reliable and efficient method for isolating pure, viable CD4+ T cells from whole blood. This kit uses an immunomagnetic separation technique with specific CD4-conjugated beads, ensuring the targeted isolation of CD4+ cells while removing non-CD4+ cells and debris. The isolated CD4+ cells are then released from the magnetic beads. The protocol is quick and straightforward, designed to be completed in under 30 minutes with minimal hands-on time. It is optimized for processing up to 30 mL of blood, with all necessary reagents (CD4 conjugate, magnetic spheres, isolation buffer, and release buffer) included in the kit. The isolated CD4+ cells are ideal for downstream applications, including flow cytometry, gene expression analysis, and immunological assays. The ReadiPrep™ CD4+ Cell Isolation Kit offers high reproducibility and purity, making it an ideal choice for researchers studying immune responses, T cell activation, or conducting clinical trials.

References

View all 50 references: Citation Explorer

Assessment of Cell Isolation From Human Milk Using Immunomagnetic Beads.

Authors:

Radhi, Noor and Paul, Ayamita and Muelbert, Mariana and Toldi, Gergely

Journal:

Journal of human lactation : official journal of International Lactation Consultant Association (2025): 8903344251316491

Unlocking novel T cell-based immunotherapy for hepatocellular carcinoma through neoantigen-driven T cell receptor isolation.

Authors:

Maravelia, Panagiota and Yao, Haidong and Cai, Curtis and Nascimento Silva, Daniela and Fransson, Jennifer and Nilsson, Ola B and Lu, Yong-Chen William and Micke, Patrick and Botling, Johan and Gatto, Francesca and Rovesti, Giulia and Carlsten, Mattias and Sallberg, Matti and Stål, Per and Jorns, Carl and Buggert, Marcus and Pasetto, Anna

Journal:

Gut (2025)

Neoantigen-specific stimulation of tumor-infiltrating lymphocytes enables effective TCR isolation and expansion while preserving stem-like memory phenotypes.

Authors:

Levin, Noam and Kim, Sanghyun P and Marquardt, Charles A and Vale, Nolan R and Yu, Zhiya and Sindiri, Sivasish and Gartner, Jared J and Parkhurst, Maria and Krishna, Sri and Lowery, Frank J and Zacharakis, Nikolaos and Levy, Lior and Prickett, Todd D and Benzine, Tiffany and Ray, Satyajit and Masi, Robert V and Gasmi, Billel and Li, Yong and Islam, Rafiqul and Bera, Alakesh and Goff, Stephanie L and Robbins, Paul F and Rosenberg, Steven A

Journal:

Journal for immunotherapy of cancer (2024)

Loneliness and social isolation in people with HIV aged ≥50 years. The No One Alone (NOA)-GeSIDA study conducted by the GeSIDA 12021 study group.

Authors:

Blanco, José-Ramón and Gonzalez-Baeza, Alicia and Martinez-Vicente, Ana and Albendin-Iglesias, Helena and De La Torre, Javier and Jarrin, Inma and González-Cuello, Inmaculada and Cabello-Clotet, Noemí and Barrios-Blandino, Ana-María and Sanjoaquin-Conde, Isabel and Montes-Ramirez, Mª-Luisa and Melus, Estrella and Pérez-Esquerdo, Verónica and Tomas-Jimenez, Cristina and Saumoy-Linares, María and Lopez-Lirola, Ana-Mª and Hidalgo-Tenorio, Carmen and Muelas-Fernandez, Magdalena and Galindo-Puerto, Mª-José and Abadía, Jessica and Manzanares, Eduardo and Segundo-Martin, Cristina and Fernandez-Lopez, Mª-Angeles and Barrios-Vega, María and De Miguel, Marta and Olalla, Julian and ,

Journal:

HIV medicine (2024)

Methodological optimisation of thymocyte isolation and cryopreservation of human thymus samples.

Authors:

Hagen, Ruth R and Xu, Calvin and Koay, Hui-Fern and Konstantinov, Igor E and Berzins, Stuart P and Kedzierska, Katherine and van de Sandt, Carolien E

Journal:

Journal of immunological methods (2024): 113651

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on December 15, 2025

Component A: ReadiPrep™ CD4 Conjugate	One vial (0.75mL)
Component B: Magnetic Spheres	One vial (1 mL )
Component C: Isolation Buffer	One bottle, ~25 mL
Component D: Release Buffer	One bottle, ~30 mL