Name: ReadiPrep™ Dead Cell Removal Kit
Brand: AAT Bioquest
SKU: 67300
Price: 595 USD
Availability: InStock

ReadiPrep™ Dead Cell Removal Kit

ReadiPrep™ Dead Cell Removal Kit efficiently enriches live cell populations for high-quality downstream applications.

Effective dead cell removal: Enrich live cell populations by removing dead cells with high efficiency
Applications: Suitable for flow cytometry, live cell culture, viability studies, and apoptosis research
Quick workflow: Simple incubation and magnetic separation steps minimize processing time
Comparable alternative: Provides functionality similar to Miltenyi Biotec’s Dead Cell Removal Kits with optimized Annexin V-based specificity for improved accuracy

Starting with 72 hours cultured Jurkat cells, which has 56% live cells and 41.5% apoptosis cells (Annexin V positive), the live cell content is enriched to 94% after removal of dead cells with ReadiPrep™ Dead Cell Removal Kit (Cat#67300).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
67300	1x10^9 cells	Price

Overview

The ReadiPrep™ Dead Cell Removal Kit provides a fast and reliable method for enriching live cell populations by removing dead cells, including both apoptotic and necrotic cells, from your samples. This kit leverages Annexin V conjugation to specifically bind dead cells, followed by magnetic separation using magnetic spheres, ensuring that live cells are efficiently isolated from dead ones. This kit is designed for ease of use with minimal hands-on time. The provided reagents and protocol can help to quickly obtain enriched live cell populations from a variety of sample types, such as cultured cells or tissue homogenates. The resulting live cell suspension is highly pure, making it ideal for downstream applications like flow cytometry, cell culture, or molecular analysis making it ideal for studies involving cell viability, apoptosis, or cellular responses to drug treatments.

References

View all 15 references: Citation Explorer

Enrichment of thawed boar spermatozoa with an intact membrane using Magnetic Activated Cell Sorting.

Authors:

Johannisson, Anders and Morrell, Jane M and Wallgren, Margareta

Journal:

Animal reproduction science (2024): 107493

The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.

Authors:

Li, Weifeng and Gurdziel, Katherine and Pitchaikannu, Ahalya and Gupta, Naman and Hazlett, Linda D and Xu, Shunbin

Journal:

The ocular surface (2023): 17-41

Improving cell viability using counterflow centrifugal elutriation.

Authors:

Li, Anqi and Barabadi, Mehri and McDonald, Hannah and Chan, Siow Teng and Krause, Mirja and Ooi, Joshua D and Kusuma, Gina D and James, David and Lim, Rebecca

Journal:

Cytotherapy (2022): 650-658

The Culture Dish Surface Influences the Phenotype and Dissociation Strategy in Distinct Mouse Macrophage Populations.

Authors:

Song, Qiaoling and Zhang, Yazhuo and Zhou, Mingming and Xu, Yuting and Zhang, Qianyue and Wu, Lihong and Liu, Shan and Zhang, Minghui and Zhang, Lei and Wu, Zhihua and Peng, Weixun and Liu, Xutao and Zhao, Chenyang

Journal:

Frontiers in immunology (2022): 920232

Continuous removal of small nonviable suspended mammalian cells and debris from bioreactors using inertial microfluidics.

Authors:

Kwon, Taehong and Yao, Rujie and Hamel, Jean-François P and Han, Jongyoon

Journal:

Lab on a chip (2018): 2826-2837

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22

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Email	sales@aatbio.com
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Shipping	Standard overnight for United States, inquire for international

Page updated on September 30, 2025

Component A: ReadiPrep™ Dead Cell (Annexin V) Conjugate	One vial (500 µL)
Component B: Magnetic Spheres (2%)	One vial (1 mL )
Component C: Isolation Buffer	One bottle (10 mL)