ReadiPrep™ Endoplasmic Reticulum (ER) Isolation Kit is a complete reagent system designed for the enrichment and purification of endoplasmic reticulum from mammalian tissues and cultured cells. The ER is essential for various cellular processes including protein synthesis, calcium storage, lipid metabolism, and membrane protein trafficking. This kit enables rapid isolation of intact ER structures including rough and smooth ER without the need for ultracentrifugation.

The protocol yields multiple levels of ER enrichment, from crude microsomes to calcium-precipitated and density-gradient purified fractions. The resulting ER fractions are functionally intact and well suited for studies involving protein synthesis, degradation pathways, ER-resident enzymes, and organelle-specific biochemical assays. ReadiPrep™ ER Isolation Kit simplifies ER purification into a streamlined workflow, making it ideal for routine laboratory use.