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ReadiPrep™ Lipopolysaccharide (LPS) Isolation Kit
The ReadiPrep™ Lipopolysaccharide (LPS) Isolation Kit provides a safe and effective way to isolate LPS from the outer membrane of Gram-negative bacteria. It utilizes a bacterial membrane lysis buffer and enzymatic protein digestion to extract pure LPS from bacterial cultures, which can be quantified using carbohydrate detection methods. Unlike conventional approaches that rely on hazardous organic chemicals like chloroform or phenol, this kit provides a safer and more time-efficient alternative for isolating high-purity LPS for research applications. Lipopolysaccharides, or LPS, are found in the outer membrane of Gram-negative bacteria. It is a carbohydrate with a low molecular weight of 10-20 kDa. It is a complex molecule made of heterogeneous components comprising O antigen, core oligosaccharide, Lipid A, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, characterized by its multiple fatty acid chains, anchors the LPS into the bacterial membrane and contributes to the toxicity of the Gram-negative bacteria. Known as endotoxins, LPS is notorious for inducing potent inflammatory responses and sepsis when consumed by animals.
Bacterial lysate, prepared using the ReadiPrep™ Lipopolysaccharide (LPS) Isolation Kit, was boiled at 85 °C for 3-5 minutes in 1X loading buffer. The sample was then loaded onto a 4-12% SDS-PAGE gel and run for 70 minutes at 110V. Afterward, the gel was stained with Coomassie Blue to visualize the proteins.
Bacterial lysate, prepared using the ReadiPrep™ Lipopolysaccharide (LPS) Isolation Kit, was boiled at 85 °C for 3-5 minutes in 1X loading buffer. The sample was then loaded onto a 4-12% SDS-PAGE gel and run for 70 minutes at 110V. Afterward, the gel was stained with Coomassie Blue to visualize the proteins.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
6020110 Preps
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12352200
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on December 15, 2025