ReadiPrep™ Mitochondrial/Cytoplasmic Fractionation Kit

The ReadiPrep™ Mitochondrial/Cytoplasmic Fractionation Kit offers a convenient method for isolating mitochondria and cytoplasmic fractions from mammalian cells or tissue. The isolated mitochondria are intact and can be positively stained with a mitochondria marker (such as MitoLite™ Green). The isolated mitochondria preserve their biological activities and are compatible with many downstream applications including the study of mitochondrial respiration, mitochondria membrane potential, apoptosis, mtDAN and mtRNA, and mitochondrial protein profiling etc. The kit offers two options for the isolation of mitochondria. One option utilizes a reagent-based method which allows multiple sample preparation at the same time. The second option utilizes Dounce homogenization.

$Mitochondria fractions from HeLa cells were collected using ReadiPrep™ Mitochondrial/Cytoplasmic Fractionation Kit. The protein was quantified by Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit (#11100). 100 µg of mitochondria fragment was incubated with or without MitoLite™ Green (#22675) and assayed with a NovoCyte 3000 flow cytometer.$

Protocol

SDS

COA

Catalog	Size	Price	Quantity
60005	50 Tests	Price

Citations

View all 2 citations: Citation Explorer

Involvement of Ferroptosis Induction and Oxidative Phosphorylation Inhibition in the Anticancer-Drug-Induced Myocardial Injury: Ameliorative Role of Pterostilbene

Authors:

Fujii, Kiyomu and Fujiwara-Tani, Rina and Nukaga, Shota and Ohmori, Hitoshi and Luo, Yi and Nishida, Ryoichi and Sasaki, Takamitsu and Miyagawa, Yoshihiro and Nakashima, Chie and Kawahara, Isao and others,

Journal:

International Journal of Molecular Sciences (2024): 3015

Phosphorylation of Cofilin-1 Enhances Paclitaxel Resistance of Epithelial Ovarian Cancer Cells by Inhibiting Apoptosis

Authors:

Min, LI and Dong, Xu Dong and Lyu, Qiu Bo and Zhang, Wei and Huang, Shuai and Yang, Chun Xue and Di, CUI and Lai, Hui Ying

Journal:

Biomedical and Environmental Sciences (2021): 465--477

References

View all 2 references: Citation Explorer

Studied by Rapid Fractionation of Barley (Hordeum vulgare) Protoplasts

Authors:

Gardestrom, P.; Wigge, B., Influence of Photorespiration on ATP/ADP Ratios in the Chloroplasts, Mitochondria, and Cytosol, undefined

Journal:

Plant Physiol (1988): 69-76

and mitochondria

Authors:

Lilley, R. M.; Stitt, M.; Mader, G.; Heldt, H. W., Rapid fractionation of wheat leaf protoplasts using membrane filtration : the determination of metabolite levels in the chloroplasts, cytosol

Journal:

Plant Physiol (1982): 965-70

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

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Page updated on September 25, 2025

Component A: Isolation Buffer	1 bottle (100 mL)
Component B: Reagent A	1 vial (1 mL)