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ReadiPrep™ PEG Virus Precipitation Kit

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Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


The ReadiPrep™ PEG Virus Precipitation Kit provides a streamlined approach for the concentration of viral particles and the elimination of contaminants, circumventing the need for ultracentrifugation. This kit is well-suited for both small-scale laboratory samples with low viral titers and large-scale virus preparations with high yields and titers. It can concentrate a diverse array of viruses, including retroviruses, baculoviruses, lentiviruses, and phages, from cell culture media and environmental samples, with a concentration increase of over 100-fold. The kit includes an optimized ReadiPrep™ Virus Resuspension Solution that enhances viral recovery by 40-100%, depending on the virus type and source. The use of non-toxic reagents in the procedure ensures safe handling. The concentrated virus can be utilized in various applications, such as infection assays and viral nucleic acid purification, making this kit an invaluable tool in virological research and applications.


Example protocol


Important Note

The procedure is designed for 10 mL of virus solution, but one can adjust the volumes proportionally based on the sample size.

  1. Infect cells or transfect cells, and allow maximum virus accumulation.

  2. For mammalian cell-virus or insect-baculovirus, centrifuge culture at 3,200 X g (for bacterial phage, centrifuge at 16,000 X g) for 15 minutes at 4 °C to remove cell debris.

  3. Collect supernatant, and add 2.5 mL of ReadiPrep™ 5X PEG Solution (Component A) to 10 mL of virus supernatant.

  4. Refrigerate overnight (stable up to 2 days at 4 °C).

  5. Centrifuge at 3,200 X g for 30 minutes at 4 °C.

  6. Remove and discard the supernatant by aspiration carefully. The beige or white pellet is the virus.

  7. Add 100 uL of ReadiPrep™ Virus Resuspension Solution (Component B) to the virus pellet, and mix well.

  8. Aliquot the virus and store it at < –70 °C for future use.

    Note: For a high-titer virus preparation, the resuspension volume should be limited to about three times the volume of the white pellet, usually 1/10 to 1/100 the volume of the original sample. If insoluble material is present in the viral suspension, it can be removed by centrifuge at 3,200 x g for 15 min at +4 °C.

    Note: Avoid freeze/thaw cycles to maximize virus recovery.

    Note: Trace amounts of PEG in the virus suspension will not affect the use of the concentrated virus. In some cases, PEG may increase virus infection efficiency. However, if it is desired, the trace amount of PEG can be removed by the following procedure:

    1. Add 1 volume of solution containing 4 M KCl and 50 mM Tris-HCl, pH 7.2 (not provided), to 3 volumes of the concentrated virus suspension.  Alternatively, add solid KCl into the virus suspension to a final concentration of 1 M.

    2. Let stand on ice for 15–30 minutes. Then, spin at 12,000 x g for 10 min at 4 °C to remove the precipitate.

    3. Collect the virus supernatant carefully. And aliquot and store at < –70° C for future use.


View all 16 references: Citation Explorer
Preparation, Purification and Performance Evaluation of Polyclonal Antibody Against SARS-CoV-2 Produced in Rat.
Authors: Yaghoobizadeh, Fatemeh and Roayaei Ardakani, Mohammad and Ranjbar, Mohammad Mehdi and Khosravi, Mohammad and Galehdari, Hamid
Journal: Advanced pharmaceutical bulletin (2023): 563-572
Comparison of the methods for isolation and detection of SARS-CoV-2 RNA in municipal wastewater.
Authors: Lucansky, Vincent and Samec, Marek and Burjanivova, Tatiana and Lukacova, Eva and Kolkova, Zuzana and Holubekova, Veronika and Turyova, Eva and Hornakova, Andrea and Zaborsky, Tibor and Podlesniy, Petar and Reizigova, Lenka and Dankova, Zuzana and Novakova, Elena and Pecova, Renata and Calkovska, Andrea and Halasova, Erika
Journal: Frontiers in public health (2023): 1116636
Concentration of infectious SARS-CoV-2 by polyethylene glycol precipitation.
Authors: Alexander, Marina R and Rootes, Christina L and van Vuren, Petrus Jansen and Stewart, Cameron R
Journal: Journal of virological methods (2020): 113977
Creation of a High-Yield AAV Vector Production Platform in Suspension Cells Using a Design-of-Experiment Approach.
Authors: Zhao, Huiren and Lee, Ki-Jeong and Daris, Mark and Lin, Yun and Wolfe, Thomas and Sheng, Jackie and Plewa, Cherylene and Wang, Songli and Meisen, W Hans
Journal: Molecular therapy. Methods & clinical development (2020): 312-320
Characterization of rVSVΔG-ZEBOV-GP glycoproteins using automated capillary western blotting.
Authors: Minsker, Kevin and Rustandi, Richard R and Ha, Sha and Loughney, John W
Journal: Vaccine (2020): 7166-7174
A virus precipitation method for concentration & detection of avian influenza viruses from environmental water resources & its possible application in outbreak investigations.
Authors: Pawar, Shailesh D and Keng, Sachin S and Tare, Deeksha S and Thormothe, Anil L and Sapkal, Gajanan N and Anukumar, B and Lole, Kavita S and Mullick, Jayati and Mourya, Devendra T
Journal: The Indian journal of medical research (2019): 612-619
Two-Step Concentration of Complex Water Samples for the Detection of Viruses.
Authors: Farkas, Kata and McDonald, James E and Malham, Shelagh K and Jones, Davey L
Journal: Methods and protocols (2018)
Strategy for assessment of the colloidal and biological stability of H1N1 influenza A viruses.
Authors: Hämmerling, Frank and Lorenz-Cristea, Oliver and Baumann, Pascal and Hubbuch, Jürgen
Journal: International journal of pharmaceutics (2017): 80-87
Porcine parvovirus flocculation and removal in the presence of osmolytes.
Authors: Gencoglu, Maria F and Pearson, Eric and Heldt, Caryn L
Journal: Journal of biotechnology (2014): 83-90
A polymerase chain reaction-based method for the detection of hepatitis A virus in produce and shellfish.
Authors: Goswami, B B and Kulka, Michael and Ngo, Diana and Istafanos, Phillip and Cebula, Thomas A
Journal: Journal of food protection (2002): 393-402