The ReadiPrep™ Plasma Membrane Protein Extraction Kit is designed for efficient and reproducible isolation of plasma membrane proteins from cultured mammalian cells. Leveraging a detergent-based phase separation system, the kit selectively enriches plasma membrane proteins while minimizing contamination from cytosolic and nuclear components. The protocol involves sequential lysis, temperature-controlled incubations, and phase separations that yield a fraction containing plasma membrane proteins, thus offering lower viscosity option compared to conventional plasma membrane protein extraction methods. The included buffers are optimized for maintaining protein integrity and compatibility with downstream analytical techniques such as Western blotting, proteomics, and functional assays. This kit is ideal for researchers studying membrane-bound receptors, transporters, or surface markers and suitable for both routine and high-throughput workflows.