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ReadiPrep™ Plasmid Preparation Column
Product key features
The ReadiPrep™ Plasmid Preparation Column is a simple and efficient tool for plasmid DNA extraction from bacterial cultures.
- High Purity: Yields high-quality plasmid DNA free of RNA, proteins, and other contaminants.
- Convenient: Pre-packed, ready-to-use columns for streamlined workflows.
- Flexible: Ideal for mini-prep scale plasmid isolation from a variety of bacterial strains.
- Fast: Complete plasmid purification in under 30 minutes.
Product description
The ReadiPrep™ Plasmid Preparation Column is designed for rapid and reliable isolation of plasmid DNA from cultured bacterial cells. Using a silica-based membrane, the column selectively binds plasmid DNA under optimized buffer conditions while impurities such as RNA, proteins, and genomic DNA are washed away. These columns are compatible with Qiagen Miniprep plasmid kits.
Each column comes pre-packed and ready-to-use, minimizing prep time and improving reproducibility across samples. The protocol is simple and requires minimal equipment, making it ideal for everyday lab use. Purified plasmid DNA is suitable for a wide range of downstream applications including restriction digestion, transformation, PCR, qPCR, and sequencing.
Example protocol
AT A GLANCE
Resuspend the bacterial pellet in resuspension buffer.
Add equal volume of lysis buffer, mix gently, followed by addition of neutralization buffer and centrifuge to pellet out the debris.
Load the supernatant onto the ReadiPrep™ Plasmid Preparation Column, spin, and discard the flow through.
Wash the column with binding buffer, followed by wash buffer.
Elute the plasmid in elution buffer.
SAMPLE EXPERIMENTAL PROTOCOL
Refer to the protocol provided with the ReadiPrep™ Plasmid Preparation Mini Kit (Cat No. 67307) for detailed instructions and guidelines.
References
Authors: Yu, Minglei and Yu, Mengran and Qian, Feng
Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2025): 124381
Authors: Matsuyama, Makoto and Iwamiya, Takahiro
Journal: PloS one (2024): e0309566
Authors: Kates, Patrick A and Cook, Jordan N and Ghan, Ryan and Nguyen, Huey J and Sitasuwan, Pongkwan and Lee, L Andrew
Journal: SLAS technology (2023): 243-250
Authors: Martínez, Juan and Lampaya, Verónica and Larraga, Ana and Magallón, Héctor and Casabona, Diego
Journal: Frontiers in molecular biosciences (2023): 1248511
Authors: Ai, Yaqian and Li, Xingjiang and Wu, Xuefeng and Montalbán-López, Manuel and Zheng, Zhi and Mu, Dongdong
Journal: Enzyme and microbial technology (2023): 110191
Safety data sheet