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AAT Bioquest

ReadiPrep™ Spin Column for PCR Product Cleanup *Compatible with Qiagen QIAQuick PCR Cleanup Kit*

Product key features

ReadiPrep™ Spin Column for PCR Product Cleanup offers high-quality DNA purification for molecular biology workflows.

  • Fast and efficient: Purifies DNA from PCR and enzymatic reactions in minutes
  • Applications: Suitable for sequencing, cloning, ligation, and qPCR
  • High purity: Removes primers, nucleotides, salts, and enzymes for clean DNA recovery
  • Comparable alternative: Reliable replacement for QIAquick® PCR Purification Kit from Qiagen

Product description

The ReadiPrep™ Spin Column for PCR Product Cleanup provides a fast and efficient solution for purifying DNA from PCR reactions and other enzymatic processes. Utilizing a silica-based membrane, these spin columns selectively bind DNA while removing primers, unused nucleotides, salts, enzymes, and other contaminants, ensuring high-quality DNA recovery for sensitive downstream applications.

With a streamlined, minimal-hands-on protocol, the kit enables quick and reproducible purification in just minutes. It is compatible with a variety of sample types and delivers DNA ready for a wide range of applications, including DNA sequencing, cloning, ligation, and quantitative PCR (qPCR).

Example protocol

AT A GLANCE

  1. Add 5 volumes of binding buffer into PCR sample.
  2. Apply the mix to ReadiPrep™ Spin Column and centrifuge the column.
  3. Wash the column with wash buffer.
  4. Elute the sample from the column with elution buffer.
Note: Binding, wash and elution buffers are not provided in #60510.

SAMPLE EXPERIMENTAL PROTOCOL

Refer the protocol of the ReadiPrep™ PCR Purification Kit (cat #60511)
https://www.aatbio.com/products/readiprep-pcr-purification-kit?unit=60511

Page updated on August 13, 2025

Ordering information

Price
Unit size
Catalog Number60510
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
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Physical properties

Solvent

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Room temperature (10-25 °C)
The 18S rRNA PCR product was purified using the ReadiPrep™ Spin Column for PCR Cleanup (Cat# 60510) or a comparable column from Competitor A. The purified products were electrophoresed on a 1% agarose gel in 1X TAE buffer and subsequently stained with Gelite Safe DNA Gel Stain (Cat# 17700).
The 18S rRNA PCR product was purified using the ReadiPrep™ Spin Column for PCR Cleanup (Cat# 60510) or a comparable column from Competitor A. The purified products were electrophoresed on a 1% agarose gel in 1X TAE buffer and subsequently stained with Gelite Safe DNA Gel Stain (Cat# 17700).
The 18S rRNA PCR product was purified using the ReadiPrep™ Spin Column for PCR Cleanup (Cat# 60510) or a comparable column from Competitor A. The purified products were electrophoresed on a 1% agarose gel in 1X TAE buffer and subsequently stained with Gelite Safe DNA Gel Stain (Cat# 17700).