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ReadiScript™ RT Reverse Transcription Kit

The ReadiScript™ RT Reverse Transcription kit is a convenient tool for cDNA synthesis used in gene expression analysis with qPCR. The two-tube kit format helps to set up the reaction faster and is optimized to yield cDNA over a broad dynamic range. The 5X supermix contains a unique blend of oligo(dT) and random primer mix to prevent the 5’ and 3’ bias of the target genes. The reaction mix contains a potent blend of RNase inhibitors to protect RNA during the setup and reverse transcription process. The resulting cDNA product is compatible with all qPCR chemistries or conventional end-point RT-PCR.

GAPDH gene expression analysis using TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*. Total RNA was extracted from HeLa cells, and cDNA was amplified in replicate reactions (1000, 100, 10, 1, 0 ng- From left to right) using the ReadiScript™ RT Reverse Transcription Kit. 2 µL volumes were used for each reaction for the qPCR detection of GAPDH.
GAPDH gene expression analysis using TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*. Total RNA was extracted from HeLa cells, and cDNA was amplified in replicate reactions (1000, 100, 10, 1, 0 ng- From left to right) using the ReadiScript™ RT Reverse Transcription Kit. 2 µL volumes were used for each reaction for the qPCR detection of GAPDH.
CatalogSize
Price
Quantity
6010050 Reactions
Price
 
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
Instrument settings

Other instrumentsThermal Cycler
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Page updated on October 7, 2025