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ReadiScript™ RT Reverse Transcription Kit

The ReadiScript™ RT Reverse Transcription kit is a convenient tool for cDNA synthesis used in gene expression analysis with qPCR. The two-tube kit format helps to set up the reaction faster and is optimized to yield cDNA over a broad dynamic range. The 5X supermix contains a unique blend of oligo(dT) and random primer mix to prevent the 5’ and 3’ bias of the target genes. The reaction mix contains a potent blend of RNase inhibitors to protect RNA during the setup and reverse transcription process. The resulting cDNA product is compatible with all qPCR chemistries or conventional end-point RT-PCR.

GAPDH gene expression analysis using TAQuest™ qPCR Master Mix with Helixyte™ Green *Low ROX*. Total RNA was extracted from HeLa cells, and cDNA was amplified in replicate reactions (1000, 100, 10, 1, 0 ng- From left to right) using the ReadiScript™ RT Reverse Transcription Kit. 2 µL volumes were used for each reaction for the qPCR detection of GAPDH.
CatalogSize
Price
Quantity
6010050 Reactions
Price
 
Usage and storage

Intended useResearch Use Only (RUO)
Instrument settings

Other instrumentsThermal Cycler
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Page updated on August 3, 2026