ReadiUse™ 1 Kb Plus DNA Ladder

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

ReadiUse™ 1 kb Plus DNA ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. It is composed of 26 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100 and 50 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains four enhanced bands (3k, 1.2k, 500 and 200 bp) for easy reference. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are added for real time monitoring.

Example protocol

SAMPLE EXPERIMENTAL PROTOCOL

ReadiUse™ 1 Kb Plus DNA Ladder is pre-formulated and ready to use. 5 µL per well is recommended to use for each gel.

Images

Figure 1. ReadiUse™ 1 Kb Plus DNA Ladder (5 uL/well) was run on 1.2% agarose gel with 1 X TBE Buffer.

References

View all 15 references: Citation Explorer

The pPSU Plasmids for Generating DNA Molecular Weight Markers.
Authors: Henrici, Ryan C and Pecen, Turner J and Johnston, James L and Tan, Song
Journal: Scientific reports (2017): 2438

Capillary electrophoresis combining three-step multiplex polymerase chain reactions for diagnosing α-thalassemia.
Authors: Chen, Yen-Ling
Journal: Electrophoresis (2011): 379-85

Antitumor activity of decoy oligodeoxynucleotides targeted to NF-kappaB in vitro and in vivo.
Authors: Wang, Tao and Li, Qing-Hua and Hao, Gang-Ping and Zhai, Jing
Journal: Asian Pacific journal of cancer prevention : APJCP (2010): 193-200

[Apoptosis of human carcinoma of mouth floor KB cells and multidrug resistant KBv200 cells induced by azide methyl anthraquinone derivative].
Authors: Ding, Yan and He, Li-rong and Cao, Ka-jia and Lu, Yu and Gu, Lian-quan and Fu, Li-wu
Journal: Yao xue xue bao = Acta pharmaceutica Sinica (2005): 22-6

Luteal phase serum cell-free DNA as a marker of failed pregnancy after assisted reproductive technology.
Authors: Hart, Elaine A and Patton, William C and Jacobson, John D and King, Alan and Corselli, Johannah and Chan, Philip J
Journal: Journal of assisted reproduction and genetics (2005): 213-7

Actinobacillus actinomycetemcomitans induces apoptosis in human monocytic THP-1 cells.
Authors: Kato, Satsuki and Sugimura, Norihiko and Nakashima, Keisuke and Nishihara, Tatsuji and Kowashi, Yusuke
Journal: Journal of medical microbiology (2005): 293-298

Evidence that DNA fragmentation in apoptosis is initiated and propagated by single-strand breaks.
Authors: Walker, P R and LeBlanc, J and Sikorska, M
Journal: Cell death and differentiation (1997): 506-15

Cytotoxicity and DNA damage associated with pyrazoloacridine in MCF-7 breast cancer cells.
Authors: Grem, J L and Politi, P M and Berg, S L and Benchekroun, N M and Patel, M and Balis, F M and Sinha, B K and Dahut, W and Allegra, C J
Journal: Biochemical pharmacology (1996): 1649-59

The evaluation and implementation of match criteria for forensic analysis of DNA.
Authors: Laber, T L and Iverson, J T and Liberty, J A and Giese, S A
Journal: Journal of forensic sciences (1995): 1058-64

Pulsed field capillary electrophoresis of multikilobase length nucleic acids in dilute methyl cellulose solutions.
Authors: Kim, Y and Morris, M D
Journal: Analytical chemistry (1994): 3081-5