Name: ReadiUse™ bacterial cell lysis buffer *5X*
Brand: AAT Bioquest
SKU: 24100
Price: 118 USD
Availability: InStock

ReadiUse™ bacterial cell lysis buffer

ReadiUse™ Bacterial Cell Lysis Buffer is a concentrated reagent for lysing bacterial cells and preparing cell extracts for biochemical analysis.

Cell Lysis: Disrupts bacterial cell membranes to release intracellular components.
Concentrated Formulation: Supplied as a 5X solution for dilution to 1X.
Simple Workflow: Requires a 15-minute incubation at room temperature.
Sample Preparation: Enables collection of cell lysates for biochemical analysis.
Applications: Suitable for bacterial NAD(P)/NAD(P)H measurements and other biochemical assays.

Bacterial cell lysis is the process of breaking down cells, which is commonly used to analyze cellular components like ribosomes, inclusion bodies, plasmids, nucleoids, and other small biomolecules. The membranes can be entirely or partially lysed, depending on the detergents used. ReadiUse™ reagents require minimal hands-on time, as this bacterial cell lysis buffer only requires a simple 5-fold dilution. It is widely used for lysing cells to quantify small biological molecules such as NAD(P)/NAD(P)H in bacteria.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
24100	10 mL	Price

Overview

Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules. Depending upon the detergents used, either all or some membranes are lysed. ReadiUse™ reagents require minimal hands-on time. This bacterial cell lysis buffer just requires a simple 5-fold dilution. It is widely used for lyzing cells for quantifying small biological molecules such as NAD(P)/NAD(P)H measurement in bacteria.

Citations

View all 37 citations: Citation Explorer

Selective PET imaging of bacterial infection using a glycosylated 18F-fluorodeoxyglucose-derived tracer

Authors:

Lee, Sang Hee and Kim, Jung Min and L{\'o}pez-{\'A}lvarez, Marina and Wadhwa, Anju and Bidkar, Anil P and Ur Rahim, Junaid and Blecha, Joseph and Flavell, Robert R and Ordonez, Alvaro A and Seo, Youngho and others,

Journal:

Nature Biomedical Engineering (2026): 1--18

Use of a modified bacterial ghost lysis system for the construction of an inactivated avian pathogenic Escherichia coli vaccine candidate

Authors:

Hu, J., Zuo, J., Chen, Z., Fu, L., Lv, X., Hu, S., Shi, X., Jing, Y., Wang, Y., Wang, Z., Mi, R., Huang, Y., Liu, D., Qi, K., Han, X.

Journal:

Vet Microbiol (2019): 48-58

FRET probe-based antibacterial susceptibility testing (F-AST) by detection of bacterial nucleases released by antibiotic-induced lysis

Authors:

Park, K., Jeong, J., Yi, S. Y., Lee, W. S., Shin, Y. B.

Journal:

Biosens Bioelectron (2019): 225-229

Phage single-gene lysis: Finding the weak spot in the bacterial cell wall

Authors:

Chamakura, K., Young, R.

Journal:

J Biol Chem (2019): 3350-3358

Viral Regulation on Bacterial Community Impacted by Lysis-Lysogeny Switch: A Microcosm Experiment in Eutrophic Coastal Waters

Authors:

Chen, X., Ma, R., Yang, Y., Jiao, N., Zhang, R.

Journal:

Front Microbiol (2019): 1763

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C)

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Page updated on October 23, 2026