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ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*

Cross linked-Allophycocyanin (CL-APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is strongly fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC is the least stable among the major phycobiliproteins, susceptible to dissociation at low concentrations including concentrations at which some assays are performed. CL-APC is chemically cross-linked between α and ß subunits, and is much more stable than APC. The crosslinked APC has improved stability in aqueous solution. However, all the commercial APC materials are sold in concentrated ammonium sulfate buffers. The commercial APC materials from other vendors require the tedious dialysis or other purifications performed before it can be used for labeling purposes. AAT Bioquest offers this ReadiUse™ APC that can be readily used for any labelings without any purifications required. Our highly purified ReadiUse™ APC facilitates the rapid APC conjugations to antibodies and other proteins such as streptavidin and other secondary reagents.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
ReadiUse™ Preactivated APC6516607300000.195

References

View all 46 references: Citation Explorer
Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59
Page updated on October 11, 2024

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Physical properties

Molecular weight

~105000

Solvent

Water

Spectral properties

Correction Factor (280 nm)

0.195

Extinction coefficient (cm -1 M -1)

730000

Excitation (nm)

651

Emission (nm)

660

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Refrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
Cross linked-Allophycocyanin (CL-APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is strongly fluorescent, with an extremely high absorptivity and a high quantum efficiency. AAT Bioquest offers this ReadiUse™ APC that can be readily used for any labelings without any purifications required.
Cross linked-Allophycocyanin (CL-APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is strongly fluorescent, with an extremely high absorptivity and a high quantum efficiency. AAT Bioquest offers this ReadiUse™ APC that can be readily used for any labelings without any purifications required.
Cross linked-Allophycocyanin (CL-APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is strongly fluorescent, with an extremely high absorptivity and a high quantum efficiency. AAT Bioquest offers this ReadiUse™ APC that can be readily used for any labelings without any purifications required.