Name: ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*
Brand: AAT Bioquest
SKU: 2503
Availability: InStock

ReadiUse™ CL-APC

Cross linked-Allophycocyanin; Ammonium Sulfate-Free

ReadiUse™ CL-APC is an ammonium sulfate-free, cross-linked fluorescent protein with enhanced stability for conjugating antibodies and other biomolecules for flow cytometry.

Fluorescence Detection: Excitation at 651 nm and emission at 660 nm.
Enhanced Stability: Cross-linked subunits improve stability in aqueous solutions.
Ammonium Sulfate-Free: Eliminates purification before conjugation.
High Brightness: Strong fluorescence with high absorptivity and quantum efficiency.
Protein Compatibility: Suitable for conjugation to antibodies, streptavidin, and other proteins.

Cross linked-Allophycocyanin (CL-APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is strongly fluorescent, with an extremely high absorptivity and a high quantum efficiency. AAT Bioquest offers this ReadiUse™ APC that can be readily used for any labelings without any purifications required.

Datasheet

Catalog	Size	Price	Quantity
2503	1 mg	Price
2504	10 mg	Price

Overview

Cross linked-Allophycocyanin (CL-APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is strongly fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC is the least stable among the major phycobiliproteins, susceptible to dissociation at low concentrations including concentrations at which some assays are performed. CL-APC is chemically cross-linked between α and ß subunits, and is much more stable than APC. The crosslinked APC has improved stability in aqueous solution. However, all the commercial APC materials are sold in concentrated ammonium sulfate buffers. The commercial APC materials from other vendors require the tedious dialysis or other purifications performed before it can be used for labeling purposes. AAT Bioquest offers this ReadiUse™ APC that can be readily used for any labelings without any purifications required. Our highly purified ReadiUse™ APC facilitates the rapid APC conjugations to antibodies and other proteins such as streptavidin and other secondary reagents.

References

View all 46 references: Citation Explorer

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120

Authors:

Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.

Journal:

J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy

Authors:

Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.

Journal:

Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin

Authors:

Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.

Journal:

Biophys J (2004): 2598

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp

Authors:

Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.

Journal:

Acta Biol Hung (2003): 113

Evaluation of Tolypothrix germplasm for phycobiliprotein content

Authors:

Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.

Journal:

Folia Microbiol (Praha) (2003): 59

Physical properties

Molecular weight	~105000
Solvent	Water (Add 100 µL of H₂O to Cat# 2503 or 1 mL to Cat# 2504 to reconstitute to 10 mg/mL in PBS)

Spectral properties

Correction factor (280 nm)	0.195
Extinction coefficient (cm ^-1 M ^-1)	730000
Excitation (nm)	651
Emission (nm)	660

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on October 28, 2026