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ReadiUse™ Preactivated APC-AF700 Maleimide

APC-AF700 is a popular tandem color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at ~700 nm. AAT Bioquest offers this preactivated APC-AF700 to facilitate the APC-AF700 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated APC-AF700 maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics.

Flow cytometry analysis of PBMCs stained with an anti-human CD4 (clone SK3) antibody that was conjugated in-house using ReadiUse™ Preactivated APC-AF700 Maleimide (#2720). Fluorescence was acquired on an Aurora spectral flow cytometer with detection in the APC-AF700–specific R6-A channel.
Flow cytometry analysis of PBMCs stained with an anti-human CD4 (clone SK3) antibody that was conjugated in-house using ReadiUse™ Preactivated APC-AF700 Maleimide (#2720). Fluorescence was acquired on an Aurora spectral flow cytometer with detection in the APC-AF700–specific R6-A channel.
CatalogSize
Price
Quantity
27201 mg
Price
 
Physical properties

Molecular weightN/A
SolventWater
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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Page updated on October 13, 2025