Name: ReadiUse™ Preactivated APC-AF700 Maleimide
Brand: AAT Bioquest
SKU: 2720
Price: 430 USD
Availability: InStock

ReadiUse™ Preactivated APC-AF700 Maleimide

ReadiUse™ Preactivated APC-AF700 Maleimide is a thiol-reactive fluorescent tandem dye for conjugating reduced antibodies and other biomolecules for multicolor flow cytometry.

Fluorescence Detection: Excitation at 651 nm and emission at ~700 nm.
Maleimide Chemistry: SMCC-activated for thiol-reactive conjugation.
Ready-to-Use: Preactivated dye simplifies antibody conjugation.
Protein Compatibility: Suitable for reduced antibodies and other thiol-containing biomolecules.
Applications: Supports fluorescent antibody labeling and multicolor flow cytometry.

Flow cytometry analysis of PBMCs stained with an anti-human CD4 (clone SK3) antibody that was conjugated in-house using ReadiUse™ Preactivated APC-AF700 Maleimide (#2720). Fluorescence was acquired on an Aurora spectral flow cytometer with detection in the APC-AF700–specific R6-A channel.

Protocol

SDS

Catalog	Size	Price	Quantity
2720	1 mg	Price

Overview

APC-AF700 is a popular tandem color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at ~700 nm. AAT Bioquest offers this preactivated APC-AF700 to facilitate the APC-AF700 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated APC-AF700 maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics.

References

View all 8 references: Citation Explorer

Bivalent EGFR-Targeting DARPin-MMAE Conjugates.

Authors:

Karsten, Lennard and Janson, Nils and Le Joncour, Vadim and Alam, Sarfaraz and Müller, Benjamin and Tanjore Ramanathan, Jayendrakishore and Laakkonen, Pirjo and Sewald, Norbert and Müller, Kristian M

Journal:

International journal of molecular sciences (2022)

Detecting and predicting neutralization of alemtuzumab responses in MS.

Authors:

Saxena, Gauri and Moore, James M and Jones, Meleri and Pryce, Gareth and Ali, Liaqat and Leisegang, Georgia R and Vijay, Vivek and Loveless, Samantha and Robertson, Neil P and Schmierer, Klaus and Giovannoni, Gavin and Gnananpavan, Sharmilee and Baker, David and Tallantyre, Emma C and Kang, Angray S

Journal:

Neurology(R) neuroimmunology & neuroinflammation (2020)

Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea.

Authors:

Bennett, Kristen and Sadler, Natalie C and Wright, Aaron T and Yeager, Chris and Hyman, Michael R

Journal:

Applied and environmental microbiology (2016): 2270-2279

Pharmacokinetic Profile and Acute Toxicological Properties of a Novel Radiosensitizer Cytosine-Phosphate-Guanosine Oligodeoxynucleotide 107 in Mice Following Intravenous and Orthotopic Administration.

Authors:

Cen, Yanyan and Li, Xiaoli and Yin, Zhiwei and Yan, Zifei and Liu, Dan and Peng, Wei and Pan, Feng and Zhou, Hong

Journal:

Nucleic acid therapeutics (2015): 254-65

Membrane labeling of coral gastrodermal cells by biotinylation: the proteomic identification of surface proteins involving cnidaria-dinoflagellate endosymbiosis.

Authors:

Li, Hsing-Hui and Huang, Zi-Yu and Ye, Shih-Png and Lu, Chi-Yu and Cheng, Pai-Chiao and Chen, Shu-Hwa and Chen, Chii-Shiarng

Journal:

PloS one (2014): e85119

Physical properties

Molecular weight	N/A
Solvent	Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on October 14, 2026