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PE and APC

Phycoerythrin (PE) and allophycocyanin (APC) are intensely bright phycobiliproteins isolated from photosynthetic organisms, such as algae and cyanobacteria. By maximizing both fluorescence quantum efficiency and molar absorptivity, PE and APC have the capacity to generate fluorescence signals far brighter than any commercially available synthetic fluorophore. When conjugated to molecules having biological specificity, such as antibodies, protein A or streptavidin, phycobiliproteins yield extraordinarily luminescent probes, that have found considerable success in fluorescence imaging applications that require high sensitivity but not photostability, primarily flow cytometry, FACS, immunophenotyping and ELISAs.

 

 

Phycoerythrin (PE)


Phycoerythrin spectra
Fluorescence spectra of phycoerythrin (PE).
Phycoerythrin (PE) is a 240 kDa phycobiliprotein isolated from red algae. It exhibits an intensely bright yellow-orange fluorescence (~25-fold brighter than fluorescein) with a molar extinction coefficient of 1,960,000 cm-1M-1 and a fluorescence quantum yield of 0.84. The fluorescence spectra of PE in its native state is characterized by three absorbance peaks, a primary absorbance maxima at 565 nm and two secondary absorbance maxima at 496 nm and 545 nm, and a single emission maxima at 574 nm. This three absorbance maxima improves compatibility with imaging instruments equipped with blue (488 nm), green (532 nm) and yellow lasers (561-568 nm). Prior to using PE, it must first be conjugated to a molecule having biological specificity, such as an antibody, streptavidin or annexin V. PE and PE tandem conjugates are typically used for flow cytometry, microarrays and ELISAs, with limited uses in immunofluorescence microscopy due to its rapid photobleaching characteristics.

Advantages of PE

  • Long-wavelength emission profiles minimizes autofluorescence from biological materials
  • Minimal fluorescence quenching contributed by the covalent binding of phycobilins to the protein backbone
  • Very high water-solubility to facilitate chemical manipulation for conjugation reactions
  • Significant Stokes shifts with resolvable emission spectra for multicolor analysis
  • Multiple sites for stable conjugation with organic and synthetic compounds such as antibodies, cyanine dyes or iFluor® dyes
  • Tandem conjugates for multicolor flow cytometry - generate a range of emission signals with a single excitation laser

 

Table 1. PE At-A-Glance

Specification
Value
Laser Lines488 nm | 532 nm | 561-568 nm
Ex (nm)564 nm
Em (nm)574 nm
Filter SetTRITC
ε¹1,960,000 (cm-1M-1)
Φ²0.84
  1. ε = molar extinction coefficient at their maximum absorption wavelength.
  2. Φ = fluorescence quantum yield in aqueous buffer (pH 7.2).

Phycoerythrin Products


GAM-PE Conjugate Flow
Flow cytometry analysis of HL-60 cells stained with 1 µg/mL Mouse IgG control (Green) or with 1 µg/mL mouse Anti-Human HLA-ABC (W6/32 mAb) (Red) and then followed by Goat Anti-Mouse IgG-RPE conjugate prepared with Buccutite™ Rapid RPE Antibody Labeling Kit (Cat No. 1310). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the RPE channel.
We offer a wide selection of PE products, including CD antibodies, secondary antibodies, streptavidin conjugates, labeling kits, PE tandem dyes and more. For expeditious labeling of PE to antibodies and other proteins use ReadiUse™ PE, a lyophilized version of PE free of ammonium sulfate buffers. The removal of ammonium sulfate eliminates tedious protein purification processes, such as dialysis, making for a quick and easy conjugation. Also available are PE tandem conjugates for multicolor flow cytometry using a singular laser source.
 

Allophycocyanin (APC)


APC Spectrum
Fluorescence spectra of allophycocyanin (APC).
Allophycocyanin (APC) is a 105 kDa phycobiliprotein found in cyanobacteria and red algae. It exhibits a bright far-red fluorescence with a molar extinction coefficient of 700,000 cm-1M-1 and a fluorescence quantum yield of 0.68. Compared to PE which has a larger extinction coefficient and quantum yield, APC is not as bright. The fluorescence spectral of APC in its native state is characterized by two absorbance peaks, a primary absorbance peak at 651 nm and a secondary absrobance peak at 625 nm, and a single emission maxima at 660 nm. This dual absorbance maxima improves compatibility with imaging instruments equipped with red lasers (633-647 nm). Just like PE, APC must first be conjugated to a molecule having biological specificity, such as an antibody, streptavidin or annexin V prior to using. APC conjugates are frequently used for flow cytometry, microarrays and ELISAs, with limited uses in immunofluorescence microscopy due to its rapid photobleaching characteristics.

Advantages of APC

  • Long-wavelength emission profiles minimizes autofluorescence from biological materials
  • Minimal fluorescence quenching contributed by the covalent binding of phycobilins to the protein backbone
  • Very high water-solubility to facilitate chemical manipulation for conjugation reactions
  • Significant Stokes shifts with resolvable emission spectra for multicolor analysis
  • Multiple sites for stable conjugation with organic and synthetic compounds such as antibodies, cyanine dyes or iFluor® dyes
  • Tandem conjugates for multicolor flow cytometry - generate a range of emission signals with a single excitation laser

 

Table 2. APC At-A-Glance

Specification
Value
Laser Lines633-647 nm
Ex (nm)651 nm
Em (nm)660 nm
Filter SetCy5
ε¹700,000 (cm-1M-1)
Φ²0.68
  1. ε = molar extinction coefficient at their maximum absorption wavelength.
  2. Φ = fluorescence quantum yield in aqueous buffer (pH 7.2).

Allophycocyanin Products


GAM-APC Conjugate Flow
Flow cytometry analysis of HL-60 cells stained with 1 µg/mL Mouse IgG-APC Control (Green) or with 1 µg/mL Anti-Human CD45-APC (Red) prepared with Buccutite™ Rapid APC Antibody Labeling Kit (Cat No. 1311). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.
We offer a wide selection of APC products, including CD antibodies, secondary antibodies, streptavidin conjugtes, labeling kits, APC tandem dyes and more. For expeditious labeling of APC to antibodies and other proteins use ReadiUse™ APC, a lyophilized version of APC free of ammonium sulfate buffers. The removal of ammonium sulfate eliminates tedious protein purification processes, such as dialysis, making for a quick and easy conjugation. Also available are APC tandem conjugates for multicolor flow cytometry using a singular laser source.
 

PE and APC Tandem Dyes


Spectra of FITC, Pe, Pe-Cy5 and Pe-Cy7.
Absorption and emission spectra of FITC (green), PE (yellow), PE-Cy5® (orange) and PE-Cy7® (red) illustrating their clear spectral separation that enables four-color analysis of cells. All four fluorophores are efficiently excited by the 488 nm argon laser line (blue). FITC can be visualized with a FITC filter set. PE can be visualized with a Cy3 filter set. PE-Cy5® and PE-Cy7® can be visualized with a Cy5 and Cy7 filter sets, respectively.
PE and APC Tandem dyes consist of two different covalently linked fluorophores, a donor (e.g. R-PE or APC) and a longer-wavelength emitting fluorescence acceptor (e.g. Cy5®, Cy7® or iFluor® 647). In this conformation, the excitation of one fluorophore (termed the "donor") results in the transfer of energy to, and the fluorescing of, the other (termed the "acceptor"). This transfer of energy from donor to acceptor occurs through Förster resonance energy transfer, or FRET. In flow cytometry, tandem dyes are ideally suited for multicolor parametric analysis of cells due to their exploitation of a single excitation source and their significantly large Stokes shifts. Figure 5 illustrates the excellent spectral separation of FITC, PE, PE-Cy5® and PE-Cy7® that enables four-color analysis of cells without extensive compensation. Moreover, all four fluorophores are efficiently and simultaneously excited by the 488 nm argon ion laser.

Recommendations for successful tandem dye usage

  • Avoid extended exposure to light, tandem dyes are high susceptible to photobleaching and should be protected from light at all times.
  • Do not freeze tandem conjugates, this can result in the denaturing of the donor
  • Be cautious, fixation and permeabilization steps can reduce tandem dye brightness
  • Use appropriate controls, such as isotype controls for background, unstained cells and single color controls for compensation when performing multicolor flow cytometry
  • Determine if compensation is necessary before analyzing data, this is due to two or more tandems with overlapping emission spectra
 

Table 3. Available PE and APC tandem dyes for multicolor flow cytometry.

Tandem Dye
Ex (nm)
Em (nm)
Stoke's Shift (nm)
Unit Size
Cat No.
PE-Texas Red Tandem495, 565615120, 501 mg2619
PE-Cy5 Tandem495, 565666171, 1011 mg2610
PE-Cy5.5 Tandem495, 565671176, 1081 mg2613
PE-Cy7 Tandem495, 565778283, 2131 mg2616
PE-iFluor® 594 Tandem495, 565606111, 411 mg2600
PE-iFluor® 610 Tandem495, 566628133, 621 mg2700
PE-iFluor® 647495, 569666171, 971 mg2702
PE-iFluor® 660 Tandem495, 565678183, 1131 mg2602
PE-iFluor® 700 Tandem495, 565708213, 1431 mg2614
PE-iFluor® 710 Tandem495, 565739244, 1741 mg2615
PE-iFluor® 750495, 566778283, 2121 mg2704
PE-iFluor® 780 2617
APC-Cy5.5 Tandem651700491 mg2622
APC-Cy7 Tandem6517791281 mg2625
APC-iFluor®A7 Tandem651782-1 mg2632
APC-iFluor® 700 Tandem651710591 mg2623
APC-iFluor® 710 Tandem 651740 1 mg2631
APC-iFluor® 750 Tandem6517781271 mg2626
APC-iFluor® 800 Tandem6518201691 mg2630
APC-XFD700 Tandem651719681 mg2624
APC-XFD750 Tandem6517761251 mg2627
PerCP-Cy5.5 Tandem4896791901 mg2650
ReadiUse™ Preactivated PE-Texas Red Tandem495, 565615120, 501 mg2583
ReadiUse™ Preactivated PE-Cy5 Tandem495, 565666171, 1011 mg2580
ReadiUse™ Preactivated PE-Cy5.5 Tandem495, 565671176, 1081 mg2581
ReadiUse™ Preactivated PE-Cy7 Tandem495, 565778283, 2131 mg2582
ReadiUse™ Preactivated PE-iFluor® 594 Tandem495, 565604109, 391 mg2584
ReadiUse™ Preactivated PE-iFluor® 647 Tandem495, 565666171, 1011 mg2577
ReadiUse™ Preactivated PE-iFluor® 660 Tandem495, 565678183, 1131 mg2579
ReadiUse™ Preactivated PE-iFluor® 700 Tandem495, 565722227, 1571 mg2585
ReadiUse™ Preactivated PE-iFluor® 750 Tandem495, 565778283, 2131 mg2578
ReadiUse™ Preactivated APC-Cy5.5 Tandem651700491 mg2586
ReadiUse™ Preactivated APC-Cy7 Tandem6517791281 mg2587
ReadiUse™ Preactivated APC-iFluor® 700 Tandem651710591 mg2570
ReadiUse™ Preactivated APC-iFluor® 750 Tandem6517911401 mg2571
ReadiUse™ Preactivated APC-iFluor® 800 Tandem6518201691 mg2572
ReadiUse™ Preactivated PerCP-Cy5.5 Tandem4896791901 mg2595

 

Product Ordering Information



PE, APC, PerCP and Tandem Dyes Labeled to CD Antibodies


The following table outlines the fluorescence properties of available phcoerythrin (PE), allophycocyanin (APC), PerCP and tandem dye labeled anti-human CD antibodies for use in flow cytometry (FACS). Phycobiliproteins are uncharacteristically bright due to their high molar extinction coefficients and quantum yields, an enviable quality when imaging low-abundance targets. However, since phycobiliprotiens photobleach rapidly, they are not recommended for microscopy. For additional information on phycobiliprotein-labeled CD antibodies and availability of other clones click on any label in the table below.

 

Table 4. Available PE, APC, PerCP and tandem dye-labeled CD antibody conjugates.

Label
Ex (nm)
Em (nm)
ε¹
Laser Line (nm)
PE5665741,960,000488 nm, 532 nm, 561-568 nm
PE-Texas Red®5676151,960,000488 nm, 532 nm, 561-568 nm
PE-Alexa Fluor® 6105676271,960,000488 nm, 532 nm, 561-568 nm
PE-Cy5®5656661,960,000488 nm, 532 nm, 561-568 nm
PE-iFluor® 6475696661,960,000488 nm, 532 nm, 561-568 nm
PE-Alexa Fluor® 7005667211,960,000488 nm, 532 nm, 561-568 nm
PE-Cy7®5667781,960,000488 nm, 532 nm, 561-568 nm
PE-iFluor® 7505667781,960,000488 nm, 532 nm, 561-568 nm
APC651660700,000633-647 nm
APC-iFluor® 700651710700,000633-647 nm
APC-Cy7®651779700,000633-647 nm
APC-Alexa Fluor® 750651785700,000633-647 nm
APC-iFluor® 750651791700,000633-647 nm
PerCP477678350,000488 nm
PerCP-Cy5.5489679350,000488 nm
  1. ε = molar extinction coefficient at their maximum absorption wavelength (Units = cm-1M-1).

Table 5. Ordering info for PE, APC and tandem dye labeled secondary antibody conjugates.

Product Name
Ex (nm)
Em (nm)
Size
Cat#
PE Goat Anti-human IgG (H+L) Antibody566574200 µg50220
PE Goat Anti-human IgG (H+L) Antibody5665741 mg50221
PE Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*566574200 µg50222
PE Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*5665741 mg50223
PE/Texas Red® Goat Anti-human IgG (H+L) Antibody567615200 µg50248
PE/Texas Red® Goat Anti-human IgG (H+L) Antibody5676151 mg50249
PE/Texas Red® Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*567615200 µg50250
PE/Texas Red® Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*5676151 mg50251
PE/Alexa Fluor® 610 Goat Anti-human IgG (H+L) Antibody567627200 µg50232
PE/Alexa Fluor® 610 Goat Anti-human IgG (H+L) Antibody5676271 mg50233
PE/Alexa Fluor® 610 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*567627200 µg50234
PE/Alexa Fluor® 610 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*5676271 mg50235
PE/Cy5 Goat Anti-human IgG (H+L) Antibody565666200 µg50224
PE/Cy5 Goat Anti-human IgG (H+L) Antibody5656661 mg50225
PE/Cy5 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*565666200 µg50226
PE/Cy5 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*5656661 mg50227
PE/iFluor® 647 Goat Anti-human IgG (H+L) Antibody569666200 µg50240
PE/iFluor® 647 Goat Anti-human IgG (H+L) Antibody5696661 mg50241
PE/iFluor® 647 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*569666200 µg50242
PE/iFluor® 647 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*5696661 mg50243
PE/Alexa Fluor® 700 Goat Anti-human IgG (H+L) Antibody566725200 µg50236
PE/Alexa Fluor® 700 Goat Anti-human IgG (H+L) Antibody5667251 mg50237
PE/Alexa Fluor® 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*566725200 µg50238
PE/Alexa Fluor® 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*5667251 mg50239
PE/Cy7 Goat Anti-human IgG (H+L) Antibody566778200 µg50228
PE/Cy7 Goat Anti-human IgG (H+L) Antibody5667781 mg50229
PE/Cy7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*566778200 µg50230
PE/Cy7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*5667781 mg50231
PE/iFluor® 750 Goat Anti-human IgG (H+L) Antibody566778200 µg50244
PE/iFluor® 750 Goat Anti-human IgG (H+L) Antibody5667781 mg50245
PE/iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*566778200 µg50230
PE/iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*5667781 mg50247
APC Goat Anti-human IgG (H+L) Antibody651660200 µg50184
APC Goat Anti-human IgG (H+L) Antibody6516601 mg50185
APC Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*651660200 µg50186
APC Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*6516601 mg50187
APC/iFluor® 700 Goat Anti-human IgG (H+L) Antibody651710200 µg50196
APC/iFluor® 700 Goat Anti-human IgG (H+L) Antibody6517101 mg50197
APC/iFluor® 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*651710200 µg50198
APC/iFluor® 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*6517101 mg50199
APC/Cy7 Goat Anti-human IgG (H+L) Antibody651779200 µg50188
APC/Cy7 Goat Anti-human IgG (H+L) Antibody6517791 mg50189
APC/Cy7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*651779200 µg50190
APC/Cy7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*6517791 mg50191
APC/Cy7® tandem-labeled goat anti-rabbit IgG (H+L)651779100 µg16873
APC/Alexa Fluor® 750 Goat Anti-human IgG (H+L) Antibody651785200 µg50192
APC/Alexa Fluor® 750 Goat Anti-human IgG (H+L) Antibody6517851 mg50193
APC/Alexa Fluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*651785200 µg50194
APC/Alexa Fluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*6517851 mg50195
APC/iFluor® 750 Goat Anti-human IgG (H+L) Antibody651791200 µg50200
APC/iFluor® 750 Goat Anti-human IgG (H+L) Antibody6517911 mg50201
APC/iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*651791200 µg50202
APC/iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*6517911 mg50203

Table 6. Ordering info for PE, APC and tandem dye labeled streptavidin conjugates.

Product Name
Ex (nm)
Em (nm)
Size
Cat#
RPE-streptavidin conjugate566574100 µg16900
RPE-streptavidin conjugate5665741 mg16901
RPE-iFluor® 647-streptavidin conjugate566666100 µg16906
RPE-Cy7-streptavidin conjugate566778100 µg16916
RPE-Cy7-streptavidin conjugate5667781 mg16917
RPE-iFluor® 750-streptavidin conjugate566778100 µg16907
APC-streptavidin conjugate651660100 µg16902
APC-iFluor® 750-streptavidin conjugate566791100 µg16908

Table 7. Ordering info for PE, APC and tandem dyes for labeling antibodies, proteins and other biomolecules.

Product Name
Ex (nm)
Em (nm)
Size
Cat No.
ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free*5655751 mg2500
ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free*56557510 mg2501
ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*6516621 mg2503
ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*65166210 mg2504
CL-APC [Cross linked-AlloPhycocyanin]65166210 mg2549
CL-APC [Cross linked-AlloPhycocyanin]65166250 mg2550
CL-APC [Cross linked-AlloPhycocyanin]651662100 mg2551
CL-APC [Cross linked-AlloPhycocyanin]6516621 mg2552
C-PC [C-Phycocyanin] *CAS 11016-15-2* 1 mg2553
APC [Allophycocyanin]6516621 mg2554
APC [Allophycocyanin]65166210 mg2555
PE [R-Phycoerythrin] *CAS 11016-17-4*56557510 mg2556
PE [R-Phycoerythrin] *CAS 11016-17-4*565575100 mg2557
PE [R-Phycoerythrin] *CAS 11016-17-4*5655751 mg2558
PerCP [Peridinin-Chlorophyll-Protein Complex]4826781 mg2559
ReadiUse™ Preactivated PE5655751 mg2560
ReadiUse™ Preactivated APC6517131 mg2561
ReadiUse™ Preactivated PE Maleimide [Activated R-Phycoerythrin]5665741 mg2565
ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin] 6516601 mg2567
ReadiUse™ Preactivated APC-iFluor® 700 Tandem6517131 mg2570
ReadiUse™ Preactivated APC-iFluor® 750 Tandem6517791 mg2571
ReadiUse™ Preactivated PE-iFluor® 647 Tandem5656741 mg2577
ReadiUse™ Preactivated PE-iFluor® 750 Tandem5657791 mg2578
ReadiUse™ Preactivated PE-Cy5 Tandem5656701 mg2580
ReadiUse™ Preactivated PE-Cy5.5 Tandem5657001 mg2581
ReadiUse™ Preactivated PE-Cy7 Tandem5657801 mg2582
ReadiUse™ Preactivated PE-Texas Red Tandem5656001 mg2583
ReadiUse™ Preactivated PE-iFluor® 594 Tandem5656001 mg2584
ReadiUse™ Preactivated APC-Cy5.5 Tandem6517001 mg2586
ReadiUse™ Preactivated APC-Cy7 Tandem6517801 mg2587
ReadiUse™ Preactivated PerCP4826781 mg2590
PE-Cy5 Tandem5656701 mg2610
PE-Cy5.5 Tandem5657001 mg2613
PE-Cy7 Tandem5657801 mg2616
PE-Texas Red Tandem5656001 mg2619
APC-Cy5.5 Tandem6517001 mg2622
APC-Cy7 Tandem6517801 mg2625
ReadiUse™ Preactivated PE-Texas Red Maleimide5676151 mg2716
ReadiUse™ Preactivated PE-Cy5 Maleimide5656661 mg2717
ReadiUse™ Preactivated PE-Cy5.5 Maleimide5666711 mg2718
ReadiUse™ Preactivated PE-Cy7 Maleimide5667781 mg2719
ReadiUse™ Preactivated APC-AF700 Maleimide 1 mg2720
ReadiUse™ Preactivated APC-Cy5.5 Maleimide6567001 mg2721
ReadiUse™ Preactivated APC-Cy7 Maleimide6567791 mg2722
ReadiUse™ Preactivated PerCP Maleimide4826781 mg2723
ReadiUse™ Preactivated PerCP-Cy5.5 Maleimide4826951 mg2724
ReadiUse™ Preactivated PerCP-Cy7 Maleimide4827841 mg2725

Table 8. Ordering info for PE, APC and tandem dye antibody labeling kits.

Product Name
Ex (nm)
Em (nm)
Size
Cat No.
Buccutite™ Rapid PE Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*5655752 Labelings1310
Buccutite™ Rapid PE Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*5655752 Labelings1312
Buccutite™ Rapid PE-Cy5 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*5656742 Labelings1322
Buccutite™ Rapid PE-Cy5 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*5656742 Labelings1340
Buccutite™ Rapid PE-Cy5.5 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*5657002 Labelings1316
Buccutite™ Rapid PE-Cy5.5 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*5657002 Labelings1341
Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*5657802 Labelings1317
Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*5657802 Labelings1342
Buccutite™ Rapid PE-Texas Red Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*5656002 Labelings1318
Buccutite™ Rapid PE-Texas Red Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*5656002 Labelings1343
Buccutite™ Rapid APC Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*6516622 Labelings1311
Buccutite™ Rapid APC Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*6516622 Labelings1313
Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*6517132 Labelings1319
Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*6517002 Lalelings1320
Buccutite™ Rapid APC-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 100 ug Antibody Per Reaction*6517802 Labelings1321
Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*6517132 Labelings1347
Buccutite™ Rapid APC-Cy5.5 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*6517002 Labelings1350
Buccutite™ Rapid APC-Cy7 Tandem Antibody Labeling Kit *Microscale Optimized for Labeling 25 ug Antibody Per Reaction*6517802 Labelings1351