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ReadiUse™ PE
R-Phycoerythrin; Ammonium Sulfate-Free
R-Phycoerythrin (PE) is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. All the commercial PE materials are sold in concentrated ammonium sulfate buffers. The commercial PE materials from other vendors require the tedious dialysis or other purifications performed before it can be used for labeling purposes. AAT Bioquest offers this ReadiUse™ PE that can be readily used for any labelings without any purifications required. Our highly purified ReadiUse™ PE facilitates the rapid PE conjugations to antibodies and other proteins such as streptavidin and other secondary reagents.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with PE anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE specific B6-A channel.
Top) Spectral pattern was generated using a 4-laser spectral cytometer. Spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm) were used to create four distinct emission profiles, then, when combined, yielded the overall spectral signature. Bottom) Flow cytometry analysis of whole blood cells stained with PE anti-human CD4 *SK3* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the PE specific B6-A channel.
protocol
Protocol
COA
CatalogSize
Price
Quantity
25001 mg
Price
250110 mg
Price
 
Physical properties
Molecular weight~240000
SolventWater (Add 100 µL of H₂O to Cat# 2500 or 1 mL to Cat# 2501 to reconstitute to 10 mg/mL in PBS)
Spectral properties
Correction factor (280 nm)0.175
Extinction coefficient (cm -1 M -1)
1960000
Excitation (nm)565
Emission (nm)574
Quantum yield
0.82
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
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Page updated on January 23, 2025