Phycoerythrin (PE) | AAT Bioquest

ReadiUse™ Preactivated PE Maleimide

Activated R-Phycoerythrin

ReadiUse™ Preactivated PE Maleimide is prepared by reacting highly purified R-Phycoerythrin (PE) with SMCC. The NHS group of SMCC reacts with the lysine groups of PE, leaving maleimide groups available to react with free sulfhydryl groups on proteins to be conjugated. ReadiUse™ Preactivated PE Maleimide is highly purified to completely remove the unreacted SMCC, and lyophilized to a powder form. It is ready to use and will conjugate without further preparation upon mixing with sulfhydryl-containing target molecules such as reduced IgGs. This activated phycobiliprotein can be easily conjugated to antibodies and other proteins without the use of added chemical crosslinking agents. The prepared conjugates maintain the spectral characteristics of PE.

Flow cytometry analysis of whole blood cells stained with CD8 (SK1)-PE conjugate prepared with ReadiUse™ Preactivated PE Maleimide (Cat No. 2565). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the B4-A channel.

Protocol

COA

Catalog	Size	Price	Quantity
2565	1 mg	Price
2566	5 mg	Price

References

View all 24 references: Citation Explorer

Difference between mitogen-stimulated B and T cells in nonspecific binding of R-phycoerythrin-conjugated antibodies.

Authors:

Bohacova, Pavla and Kossl, Jan and Hajkova, Michaela and Hermankova, Barbora and Holan, Vladimir and Javorkova, Eliska

Journal:

Journal of immunological methods (2021): 113013

Relationship between circulating tumor-associated autoantibodies and clinical outcomes in advanced-stage NSCLC patients receiving PD-1/-L1 directed immune checkpoint inhibition.

Authors:

Tarhoni, Imad and Wakefield, Connor J and Kollipara, Revathi and Fidler, Mary Jo and Batus, Marta and Bonomi, Philip and Borgia, Jeffrey A

Journal:

Journal of immunological methods (2021): 112956

Quantification and Imaging of Antigens on Cell Surface with Lipid-Encapsulated Fluorescent Nanodiamonds.

Authors:

Hsieh, Feng-Jen and Chen, Yen-Wei and Hui, Yuen Yung and Lin, Chun-Hung and Chang, Huan-Cheng

Journal:

Micromachines (2019)

Development of bead based multiplexed immunoassay for evaluation of midkine, syndecan-1, and ANGPTL4 in patient serum.

Authors:

Tarhoni, Imad and Fhied, Cristina L and Pool, Mark and Liptay, Michael J and Bonomi, Philip and Seder, Christopher W and Borgia, Jeffrey A

Journal:

Journal of immunoassay & immunochemistry (2018): 84-98

R-phycoerythrin-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells.

Authors:

Kim, Myun Soo and Kim, Tae Sung

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2013): 452-60

Physical properties

Solvent

Water

Spectral properties

Correction factor (280 nm)	0.175
Extinction coefficient (cm ^-1 M ^-1)	1960000
Excitation (nm)	565
Emission (nm)	574
Quantum yield	0.82

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure; Desiccated
UNSPSC	12171501

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Page updated on January 23, 2025