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ReadiUse™ Preactivated APC Maleimide
Activated Allophycocyanin
ReadiUse™ Preactivated APC Maleimide is prepared by reacting highly purified allophycocyanin (APC) with SMCC. The NHS group of SMCC reacts with the lysine groups of APC, leaving maleimide groups available to react with free sulfhydryl groups on proteins to be conjugated. ReadiUse™ Preactivated APC Maleimide is highly purified to completely remove the unreacted SMCC, and lyophilized to powder. It is ready to use and will conjugate without further preparation upon mixing with sulfhydryl-containing target molecules such as reduced IgGs. The activated allophycocyanin can be easily conjugated to antibodies and other proteins without the use of added chemical crosslinking agents. These highly purified phycobiliproteins maintain their spectral characteristics of APC.
Flow cytometry analysis of whole blood cells stained with CD8 (SK1)-APC conjugate prepared with ReadiUse™ Preactivated APC Maleimide (Cat No. 2567). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the R1-A channel
Flow cytometry analysis of whole blood cells stained with CD8 (SK1)-APC conjugate prepared with ReadiUse™ Preactivated APC Maleimide (Cat No. 2567). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the R1-A channel
CatalogSize
Price
Quantity
25671 mg
Price
25685 mg
Price
 
Physical properties

SolventWater
Spectral properties

Correction factor (280 nm)0.195
Extinction coefficient (cm -1 M -1)
730000
Excitation (nm)651
Emission (nm)660
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025