ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin]

ReadiUse™ Preactivated APC Maleimide

Activated Allophycocyanin

ReadiUse™ Preactivated APC Maleimide is a thiol-reactive fluorescent protein reagent for conjugating reduced antibodies and other biomolecules for flow cytometry.

Fluorescence Detection: APC excitation at 651 nm and emission at 660 nm.
Maleimide Chemistry: SMCC-activated for thiol-reactive conjugation.
Ready-to-Use: Preactivated APC eliminates additional crosslinker activation.
Protein Compatibility: Suitable for reduced antibodies and other sulfhydryl-containing proteins.
Rapid Conjugation: Supports conjugation in 60–120 minutes at room temperature.

Flow cytometry analysis of whole blood cells stained with CD8 (SK1)-APC conjugate prepared with ReadiUse™ Preactivated APC Maleimide (Cat No. 2567). The fluorescence signal was monitored using an Aurora spectral flow cytometer in the R1-A channel

Protocol

Catalog	Size	Price	Quantity
2567	1 mg	Price
2568	5 mg	Price

Overview

ReadiUse™ Preactivated APC Maleimide is prepared by reacting highly purified allophycocyanin (APC) with SMCC. The NHS group of SMCC reacts with the lysine groups of APC, leaving maleimide groups available to react with free sulfhydryl groups on proteins to be conjugated. ReadiUse™ Preactivated APC Maleimide is highly purified to completely remove the unreacted SMCC, and lyophilized to powder. It is ready to use and will conjugate without further preparation upon mixing with sulfhydryl-containing target molecules such as reduced IgGs. The activated allophycocyanin can be easily conjugated to antibodies and other proteins without the use of added chemical crosslinking agents. These highly purified phycobiliproteins maintain their spectral characteristics of APC.

References

View all 13 references: Citation Explorer

Magnetic particles encoding a suspension probe for ultra-sensitive and quantitative determination of atrazine.

Authors:

Zhao, Zhigao and Dou, Xiaowen and Luo, Jiaoyang and Jin, Meiqi and Qin, Jiaan and Wang, Changjian and Yang, Shihai and Yang, Meihua

Journal:

Journal of pharmaceutical and biomedical analysis (2021): 113868

Non-oxidative band-3 clustering agents cause the externalization of phosphatidylserine on erythrocyte surfaces by a calcium-independent mechanism.

Authors:

Koshkaryev, Alexander and Livshits, Leonid and Pajic-Lijakovic, Ivana and Gural, Alexander and Barshtein, Gregory and Yedgar, Saul

Journal:

Biochimica et biophysica acta. Biomembranes (2020): 183231

Leukocyte-Derived Extracellular Vesicles in Blood with and without EpCAM Enrichment.

Authors:

Nanou, Afroditi and Zeune, Leonie L and Terstappen, Leon W M M

Journal:

Cells (2019)

Exogenous IL-9 Ameliorates Experimental Autoimmune Myasthenia Gravis Symptoms in Rats.

Authors:

Yao, Xiuhua and Zhao, Jiarui and Kong, Qingfei and Xie, Xiaoli and Wang, Jinghua and Sun, Bo and Xu, Lixia and Mu, Lili and Li, Hulun

Journal:

Immunological investigations (2018): 712-724

A high-throughput neutralizing assay for antibodies and sera against hepatitis E virus.

Authors:

Cai, Wei and Tang, Zi-Min and Wen, Gui-Ping and Wang, Si-Ling and Ji, Wen-Fang and Yang, Min and Ying, Dong and Zheng, Zi-Zheng and Xia, Ning-Shao

Journal:

Scientific reports (2016): 25141

Physical properties

Solvent

Water

Spectral properties

Correction factor (280 nm)	0.195
Extinction coefficient (cm ^-1 M ^-1)	730000
Excitation (nm)	651
Emission (nm)	660

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on October 25, 2026