ReadiUse™ Preactivated PerCP Maleimide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	350000
Excitation (nm)	482
Emission (nm)	678

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

N/A

Extinction coefficient (cm ^-1 M ^-1)

350000

Excitation (nm)

482

Emission (nm)

678

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). AAT Bioquest offers this preactivated PerCP maleimide to facilitate the PerCP-Cy5.5 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated PerCP maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. Its tandem conjugates (such as PerCP-Cy5.5 and PerCP-Cy7) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	350000
Excitation (nm)	482
Emission (nm)	678

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin]	651	660	700000
ReadiUse™ Preactivated PE Maleimide [Activated R-Phycoerythrin]	565	574	1960000

References

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Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.
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Journal: Cytokine (2022): 155723

Topical gel of mesenchymal stem cells-conditioned medium under TNF-α precondition accelerates wound closure healing in full-thickness skin defect animal model.
Authors: Putra, Agung and Ibrahim, Sugeng and Muhar, Adi Muradi and Kuntardjo, Novalia and Dirja, Bayu Tirta and Pasongka, Zenitalia and Tunru, Insan Sosiawan
Journal: Journal of medicine and life (2022): 214-221

Spurious CD34 expression in B-cell lymphoma due to nonspecific binding to PerCP-Cy5.5 fluorochrome conjugates: A rare phenomenon and a diagnostic pitfall.
Authors: Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu
Journal: Cytometry. Part B, Clinical cytometry (2022)

Pharmacological fatty acid synthase inhibitors differently affect the malignant phenotype of oral cancer cells.
Authors: Boelcke, Willian Peter and Teixeira, Isadora Ferrari and Aquino, Iara Gonçalves and Mazzaro, Amanda Ramos and Cuadra-Zelaya, Florence Juana Maria and de Souza, Ana Paula and Salo, Tuula and Della Coletta, Ricardo and Graner, Edgard and Bastos, Débora Campanella
Journal: Archives of oral biology (2022): 105343

Routine flow cytometry approach for the evaluation of solid tumor neoplasms and immune cells in minimally invasive samples.
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)

The hernia sac-A suitable source for obtaining mesenchymal stem cells.
Authors: Lin, Alpha Dian-Yu and Tung, Min-Che and Lu, Chin-Heng
Journal: Surgery open science (2021): 40-44

The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205

Platelet and neutrophil responses to Porphyromonas gingivalis in human whole blood.
Authors: Chen, William A and Fletcher, Hansel M and Payne, Kimberly J and Aka, Sheryl and Thornburg, Melanie B and Gheorghe, Joseph D and Safi, Shahnaj Binte and Shavlik, David and Oyoyo, Udochukwu and Boskovic, Danilo S
Journal: Molecular oral microbiology (2021): 202-213

Optimization of stimulation and staining conditions for intracellular cytokine staining (ICS) for determination of cytokine-producing T cells and monocytes.
Authors: Mandala, Wilson and Harawa, Visopo and Munyenyembe, Alinane and Soko, Monica and Longwe, Herbert
Journal: Current research in immunology (2021): 184-193

Identification of circulating cells interacted with integrin α4β1 ligand peptides REDV or HGGVRLY.
Authors: Hsu, Yu-I and Mahara, Atsushi and Yamaoka, Tetsuji
Journal: Peptides (2021): 170470