ReadiUse™ Preactivated PerCP Maleimide
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). AAT Bioquest offers this preactivated PerCP maleimide to facilitate the PerCP-Cy5.5 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated PerCP maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. Its tandem conjugates (such as PerCP-Cy5.5 and PerCP-Cy7) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin] | 651 | 660 | 730000 | 0.195 |
ReadiUse™ Preactivated PE Maleimide [Activated R-Phycoerythrin] | 565 | 574 | 1960000 | 0.175 |
References
View all 50 references: Citation Explorer
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Authors: Putra, Agung and Ibrahim, Sugeng and Muhar, Adi Muradi and Kuntardjo, Novalia and Dirja, Bayu Tirta and Pasongka, Zenitalia and Tunru, Insan Sosiawan
Journal: Journal of medicine and life (2022): 214-221
Spurious CD34 expression in B-cell lymphoma due to nonspecific binding to PerCP-Cy5.5 fluorochrome conjugates: A rare phenomenon and a diagnostic pitfall.
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Journal: Cytometry. Part B, Clinical cytometry (2022)
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Journal: Cytometry. Part B, Clinical cytometry (2022)
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Authors: Boelcke, Willian Peter and Teixeira, Isadora Ferrari and Aquino, Iara Gonçalves and Mazzaro, Amanda Ramos and Cuadra-Zelaya, Florence Juana Maria and de Souza, Ana Paula and Salo, Tuula and Della Coletta, Ricardo and Graner, Edgard and Bastos, Débora Campanella
Journal: Archives of oral biology (2022): 105343
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Journal: Cytokine (2022): 155723
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Journal: Cytometry. Part B, Clinical cytometry (2022)
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