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ReadiUse™ Preactivated PerCP Maleimide

Ordering information
Price ()
Catalog Number2723
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weightN/A
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)350000
Excitation (nm)477
Emission (nm)678
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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OverviewpdfSDSpdfProtocol


Molecular weight
N/A
Extinction coefficient (cm -1 M -1)
350000
Excitation (nm)
477
Emission (nm)
678
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). AAT Bioquest offers this preactivated PerCP maleimide to facilitate the PerCP-Cy5.5 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated PerCP maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. Its tandem conjugates (such as PerCP-Cy5.5 and PerCP-Cy7) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)350000
Excitation (nm)477
Emission (nm)678

References


View all 50 references: Citation Explorer
Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.
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Journal: Cytokine (2022): 155723
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Spurious CD34 expression in B-cell lymphoma due to nonspecific binding to PerCP-Cy5.5 fluorochrome conjugates: A rare phenomenon and a diagnostic pitfall.
Authors: Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu
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Authors: Boelcke, Willian Peter and Teixeira, Isadora Ferrari and Aquino, Iara Gonçalves and Mazzaro, Amanda Ramos and Cuadra-Zelaya, Florence Juana Maria and de Souza, Ana Paula and Salo, Tuula and Della Coletta, Ricardo and Graner, Edgard and Bastos, Débora Campanella
Journal: Archives of oral biology (2022): 105343
Routine flow cytometry approach for the evaluation of solid tumor neoplasms and immune cells in minimally invasive samples.
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
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Authors: Lin, Alpha Dian-Yu and Tung, Min-Che and Lu, Chin-Heng
Journal: Surgery open science (2021): 40-44
The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205
Platelet and neutrophil responses to Porphyromonas gingivalis in human whole blood.
Authors: Chen, William A and Fletcher, Hansel M and Payne, Kimberly J and Aka, Sheryl and Thornburg, Melanie B and Gheorghe, Joseph D and Safi, Shahnaj Binte and Shavlik, David and Oyoyo, Udochukwu and Boskovic, Danilo S
Journal: Molecular oral microbiology (2021): 202-213
Optimization of stimulation and staining conditions for intracellular cytokine staining (ICS) for determination of cytokine-producing T cells and monocytes.
Authors: Mandala, Wilson and Harawa, Visopo and Munyenyembe, Alinane and Soko, Monica and Longwe, Herbert
Journal: Current research in immunology (2021): 184-193
Identification of circulating cells interacted with integrin α4β1 ligand peptides REDV or HGGVRLY.
Authors: Hsu, Yu-I and Mahara, Atsushi and Yamaoka, Tetsuji
Journal: Peptides (2021): 170470