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ReadiUse™ Preactivated PerCP Maleimide
PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677 nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). AAT Bioquest offers this preactivated PerCP maleimide to facilitate the PerCP-Cy5.5 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated PerCP maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. Its tandem conjugates (such as PerCP-Cy5.5 and PerCP-Cy7) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin] | 651 | 660 | 730000 | - | 0.195 |
| ReadiUse™ Preactivated PE Maleimide [Activated R-Phycoerythrin] | 565 | 574 | 1960000 | 0.82 | 0.175 |
References
View all 50 references: Citation Explorer
Topical gel of mesenchymal stem cells-conditioned medium under TNF-α precondition accelerates wound closure healing in full-thickness skin defect animal model.
Authors: Putra, Agung and Ibrahim, Sugeng and Muhar, Adi Muradi and Kuntardjo, Novalia and Dirja, Bayu Tirta and Pasongka, Zenitalia and Tunru, Insan Sosiawan
Journal: Journal of medicine and life (2022): 214-221
Authors: Putra, Agung and Ibrahim, Sugeng and Muhar, Adi Muradi and Kuntardjo, Novalia and Dirja, Bayu Tirta and Pasongka, Zenitalia and Tunru, Insan Sosiawan
Journal: Journal of medicine and life (2022): 214-221
Spurious CD34 expression in B-cell lymphoma due to nonspecific binding to PerCP-Cy5.5 fluorochrome conjugates: A rare phenomenon and a diagnostic pitfall.
Authors: Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu
Journal: Cytometry. Part B, Clinical cytometry (2022)
Authors: Shi, Min and Timm, Michael M and Howard, Matthew T and Jevremovic, Dragan and Yuan, Ji and Greipp, Patricia T and Peterson, Jess F and Roh, Dana J and Horna, Pedro and Olteanu, Horatiu
Journal: Cytometry. Part B, Clinical cytometry (2022)
Pharmacological fatty acid synthase inhibitors differently affect the malignant phenotype of oral cancer cells.
Authors: Boelcke, Willian Peter and Teixeira, Isadora Ferrari and Aquino, Iara Gonçalves and Mazzaro, Amanda Ramos and Cuadra-Zelaya, Florence Juana Maria and de Souza, Ana Paula and Salo, Tuula and Della Coletta, Ricardo and Graner, Edgard and Bastos, Débora Campanella
Journal: Archives of oral biology (2022): 105343
Authors: Boelcke, Willian Peter and Teixeira, Isadora Ferrari and Aquino, Iara Gonçalves and Mazzaro, Amanda Ramos and Cuadra-Zelaya, Florence Juana Maria and de Souza, Ana Paula and Salo, Tuula and Della Coletta, Ricardo and Graner, Edgard and Bastos, Débora Campanella
Journal: Archives of oral biology (2022): 105343
Recombinant thrombomodulin attenuates hyper-inflammation and glycocalyx damage in a murine model of Streptococcus pneumoniae-induced sepsis.
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
Authors: Watanabe, Eizo and Akamatsu, Toshinobu and Ohmori, Masaaki and Kato, Mayu and Takeuchi, Noriko and Ishiwada, Naruhiko and Nishimura, Rintaro and Hishiki, Haruka and Fujimura, Lisa and Ito, Chizuru and Hatano, Masahiko
Journal: Cytokine (2022): 155723
Routine flow cytometry approach for the evaluation of solid tumor neoplasms and immune cells in minimally invasive samples.
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
Authors: Quirós-Caso, Covadonga and Arias Fernández, Tamara and Fonseca-Mourelle, Ariana and Torres, Héctor and Fernández, Luis and Moreno-Rodríguez, Maria and Ariza-Prota, Miguel Ángel and López-González, Francisco Julián and Carvajal-Álvarez, Miguel and Alonso-Álvarez, Sara and Moro-García, Marco Antonio and Colado, Enrique
Journal: Cytometry. Part B, Clinical cytometry (2022)
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