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ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*
Ordering information
| Price | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 485.41 |
| Solvent | Water |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 220001 |
| Excitation (nm) | 601 |
| Emission (nm) | 699 |
Storage, safety and handling
| H-phrase | H303, H313, H340 |
| Hazard symbol | T |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R68 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure; Desiccated |
| UNSPSC | 41116134 |
Related products
| Overview |  SDSProtocol |
See also: Immunohistochemistry (IHC)
CAS 252903-95-0 | Molecular weight 485.41 | Extinction coefficient (cm -1 M -1) 220001 | Excitation (nm) 601 | Emission (nm) 699 |
DRAQ5™ is the trade name of 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione (DRAQ-5™ is the trade mark of Biostatus Ltd). It is a far-red DNA stain for live or fixed cells, and it can be readily used for multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins. It is lipophilic and membrane permeable dye for live-cell and fixed-cell staining and quantitation of DNA content. It is also ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS). Due to its far-red excitation and emission, DRAQ-5 Stain can be multiplexed with many other fluorophores. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins. DRAQ-5 is compatible with many existing protocols across a wide range of instrumentation platforms. AAT Bioquest offers this ready to use solution to facilitate your fluorescence imaging and flow cytometric tests.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ DRAQ5 Staining Solution *5 mM in Water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 206.011 µL | 1.03 mL | 2.06 mL | 10.301 mL | 20.601 mL |
| 5 mM | 41.202 µL | 206.011 µL | 412.023 µL | 2.06 mL | 4.12 mL |
| 10 mM | 20.601 µL | 103.006 µL | 206.011 µL | 1.03 mL | 2.06 mL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Spectral properties
| Extinction coefficient (cm -1 M -1) | 220001 |
| Excitation (nm) | 601 |
| Emission (nm) | 699 |
Images
Figure 1. Live Cell Cycle analysis using DRAQ5. Jurkat cells were stained with 10uM DRAQ5 in growth medium for 15min, and analyzed with flow cytometer APC channel.
Figure 2. Chemical structure for ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*.
Citations
View all 7 citations: Citation Explorer
Preparing Planarian Cells for High-content Fluorescence Microscopy Using RNA in situ Hybridization and Immunocytochemistry
Authors: Grohme, Markus and Frank, Olga and Rink, Jochen
Journal: (2021)
Authors: Grohme, Markus and Frank, Olga and Rink, Jochen
Journal: (2021)
One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
DRAQ5 and Eosin ('D&E') as an Analog to Hematoxylin and Eosin for Rapid Fluorescence Histology of Fresh Tissues
Authors: Elfer, K. N., Sholl, A. B., Wang, M., Tulman, D. B., M and ava, S. H., Lee, B. R., Brown, J. Q.
Journal: PLoS One (2016): e0165530
Authors: Elfer, K. N., Sholl, A. B., Wang, M., Tulman, D. B., M and ava, S. H., Lee, B. R., Brown, J. Q.
Journal: PLoS One (2016): e0165530
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1
Short exposure to the DNA intercalator DRAQ5 dislocates the transcription machinery and induces cell death
Authors: Richard, E., Causse, S., Spriet, C., Fourre, N., Trinel, D., Darzacq, X., V and enbunder, B., Heliot, L.
Journal: Photochem Photobiol (2011): 256-61
Authors: Richard, E., Causse, S., Spriet, C., Fourre, N., Trinel, D., Darzacq, X., V and enbunder, B., Heliot, L.
Journal: Photochem Photobiol (2011): 256-61
The use of DRAQ5 to monitor intracellular DNA in Escherichia coli by flow cytometry
Authors: Silva, F., Lourenco, O., Pina-Vaz, C., Rodrigues, A. G., Queiroz, J. A., Domingues, F. C.
Journal: J Fluoresc (2010): 907-14
Authors: Silva, F., Lourenco, O., Pina-Vaz, C., Rodrigues, A. G., Queiroz, J. A., Domingues, F. C.
Journal: J Fluoresc (2010): 907-14
Influence of the live cell DNA marker DRAQ5 on chromatin-associated processes
Authors: Mari, P. O., Verbiest, V., Sabbioneda, S., Gourdin, A. M., Wijgers, N., Dinant, C., Lehmann, A. R., Vermeulen, W., Giglia-Mari, G.
Journal: DNA Repair (Amst) (2010): 848-55
Authors: Mari, P. O., Verbiest, V., Sabbioneda, S., Gourdin, A. M., Wijgers, N., Dinant, C., Lehmann, A. R., Vermeulen, W., Giglia-Mari, G.
Journal: DNA Repair (Amst) (2010): 848-55
References
View all 1 references: Citation Explorer
A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry
Authors: Smith PJ, Wiltshire M, Davies S, Patterson LH, Hoy T.
Journal: J Immunol Methods (1999): 131
Authors: Smith PJ, Wiltshire M, Davies S, Patterson LH, Hoy T.
Journal: J Immunol Methods (1999): 131
Application notes
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A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
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Biotin Labeling Molecules and Their Biological Applications
Buccutite™ Bioconjugation Technology
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
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Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
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What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?