ReadiUse™ NADP Regenerating Kit
Overview | ![]() ![]() |
Components
Component A: NADP Regenerating Buffer I | 1 bottle (10 mL) |
Component B: NADP Regenerating Buffer II | 1 bottle (10 mL) |
Component C: NADP Regenerating Enzyme Mix | 1 vial |
Component D: NADPH | 2 vials |
Example protocol
AT A GLANCE
Important notes
Thaw all the components at room temperature before use.
PREPARATION OF STOCK SOLUTION
1. NADPH solution (40X):
Add 0.5 mL H2O into one vial of NADPH (Component D) to make 40X NADPH solution. Note: One vial of NADPH is for making 10 mL NADP Regenerating Solution.
2. NADP Regenerating Enzyme Mix solution (400X):
Add 100 µL H2O into the vial of NADP Regenerating Enzyme Mix (Component C) to make 400X NADP Regenerating Enzyme Mix solution. Protect from light.
PREPARATION OF WORKING SOLUTION
Add 5 mL of NADP Regenerating Buffer I (Component A), 5 mL of NADP Regenerating Buffer II (Component B), 0.5 mL of 40X NADPH solution, and 50 μL of 400X NADP Regenerating Enzyme Mix solution to make a total volume of 10.55 mL 2X NADP regenerating solution. Keep from light. Note: Activated NADP regenerating solution is not stable, and should be prepared freshly before use. 10 mL of NADP Regenerating Solution is enough for 1 plate.
SAMPLE EXPERIMENTAL PROTOCOL
- Add equal volume of 2X NADP Regenerating solution into the desired assay system.
Citations
Authors: Song, Minmin and Delaplain, Patrick and Nguyen, Trang T and Liu, Xibei and Wickenberg, Leah and Jeffrey, Christopher and Blomquist, Gary J and Tittiger, Claus
Journal: Insect biochemistry and molecular biology (2014): 73--80
Authors: Song, Minmin and Gorzalski, Andrew and Nguyen, Trang T and Liu, Xibei and Jeffrey, Christopher and Blomquist, Gary J and Tittiger, Claus
Journal: Journal of chemical ecology (2014): 181--189
Authors: Song, Minmin and Kim, Amy C and Gorzalski, Andrew J and MacLean, Marina and Young, Sharon and Ginzel, Matthew D and Blomquist, Gary J and Tittiger, Claus
Journal: Insect biochemistry and molecular biology (2013): 336--343
Authors: Qiu, Yue and Tittiger, Claus and Wicker-Thomas, Claude and Le Goff, Gaelle and Young, Sharon and Wajnberg, Eric and Fricaux, Thierry and Taquet, Nathalie and Blomquist, Gary J and Feyereisen, René
Journal: Proceedings of the National Academy of Sciences (2012): 14858--14863
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines
Bio-inspired NADH regeneration by carbon nitride photocatalysis using diatom templates
Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila
EphA2 Induces Metastatic Growth Regulating Amoeboid Motility and Clonogenic Potential in Prostate Carcinoma Cells