Name: ReadiUse™ Preactivated APC-Cy5.5 Tandem
Brand: AAT Bioquest
SKU: 2586
Price: 415 USD
Availability: InStock

ReadiUse™ Preactivated APC-Cy5.5 Tandem

ReadiUse™ Preactivated APC-Cy5.5 Tandem is a ready-to-conjugate fluorescent tandem dye for labeling antibodies and other proteins for multicolor flow cytometry.

Fluorescence Detection: Excitation at 651 nm and emission at 700 nm.
Conjugation Chemistry: Buccutite™ FOL/MTA chemistry enables protein conjugation through amino-group modification.
High Conjugation Yield: Higher yields than conventional SMCC-based chemistry.
Ready-to-Use: Includes preactivated dye and Buccutite™ MTA reagent.
Efficient Labeling: Supports conjugation at lower protein concentrations than SMCC chemistry.

Our preactivated APC-Cy5.5 was premodified with our Buccutite™ FOL (provided). Your antibody (or other proteins) is modified with our Buccutite™ MTA (provided as free sample) to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC-Cy5.5 (provided) to give the desired APC-Cy5.5-antibody conjugate in much higher yield than the SMCC chemistry. In addition our preactivated APC-Cy5.5 reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.

Protocol

SDS

Catalog	Size	Price	Quantity
2586	1 mg	Price

Overview

Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC-Cy5.5 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~700 nm. AAT Bioquest offers this preactivated APC-Cy5.5 to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy5.5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy5.5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.

References

View all 46 references: Citation Explorer

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120

Authors:

Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.

Journal:

J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy

Authors:

Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.

Journal:

Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin

Authors:

Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.

Journal:

Biophys J (2004): 2598

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp

Authors:

Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.

Journal:

Acta Biol Hung (2003): 113

Evaluation of Tolypothrix germplasm for phycobiliprotein content

Authors:

Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.

Journal:

Folia Microbiol (Praha) (2003): 59

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	700

Usage and storage

Intended use

Research Use Only (RUO)

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Page updated on October 28, 2026

A: ReadiUse™ Preactivated APC-Cy5.5 Tandem	1 vial (1 mg)
B: Buccutite™ MTA	1 vial (100 μg)
C: Spin Desalting Column	Not Included