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ReadiUse™ Preactivated APC-Cy5.5 Tandem
Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics. APC-Cy5.5 is a popular color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at~700 nm. AAT Bioquest offers this preactivated APC-Cy5.5 to facilitate the APC-Cy5.5 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated APC-Cy5.5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated APC-Cy5.5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Our preactivated APC-Cy5.5 was premodified with our Buccutite™ FOL (provided). Your antibody (or other proteins) is modified with our Buccutite™ MTA (provided as free sample) to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC-Cy5.5 (provided) to give the desired APC-Cy5.5-antibody conjugate in much higher yield than the SMCC chemistry. In addition our preactivated APC-Cy5.5 reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.
Our preactivated APC-Cy5.5 was premodified with our Buccutite™ FOL (provided). Your antibody (or other proteins) is modified with our Buccutite™ MTA (provided as free sample) to give MTA-modified protein (such as antibody). The MTA-modified protein readily reacts with FOL-modified APC-Cy5.5 (provided) to give the desired APC-Cy5.5-antibody conjugate in much higher yield than the SMCC chemistry. In addition our preactivated APC-Cy5.5 reacts with MTA-modified biopolymers at much lower concentrations than the SMCC chemistry.
CatalogSize
Price
Quantity
25861 mg
Price
 
Physical properties

SolventWater
Spectral properties

Extinction coefficient (cm -1 M -1)
700000
Excitation (nm)651
Emission (nm)700
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 8, 2025