ReadiUse™ Preactivated APC-Cy7 Maleimide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | Water |
Spectral properties
Extinction coefficient (cm -1 M -1) | 700000 |
Excitation (nm) | 651 |
Emission (nm) | 779 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
Related products
Overview | ![]() ![]() |
See also: Flow Cytometry Reagents, PE and APC
Molecular weight N/A | Extinction coefficient (cm -1 M -1) 700000 | Excitation (nm) 651 | Emission (nm) 779 |
APC-Cy7 is a popular tandem color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at ~780 nm. AAT Bioquest offers this preactivated APC-Cy7 to facilitate the APC-Cy7 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated APC-Cy7 maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics.
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
Reduction of Antibody
- Prepare a fresh solution of 1.0 M DTT (15.4 mg/100 µL) in distilled water. Antibody solutions should be at 2 mg/mL or higher for best results. The reduction can be carried out in different buffers for example: MES, phosphate, and TRIS buffers (pH range 6 to 8). The antibody should be concentrated if less than 2 mg/mL.
- Add 2 µL of 1.0 M DTT stock per 100 µL of antibody solution and mix well. Let the antibody solution stand at room temp for 30 minutes without additional mixing (to minimize reoxidation of cysteines to cystines).
- Purify the reduced antibody over a desalting column pre-equilibrated with 50 mM MES Buffer (pH=6.0-6.5) with 2 mM EDTA. (Desalting column: https://www.aatbio.com/products/readiuse-bio-gel-p-6-spin-column?unit=60500)
- Measure the Antibody concentration with Nanodrop. (Con. (mg/ml)= A280nm/1.4) Note: The reduced antibody is not stable; the conjugation reaction needs to be carried out the conjugation as soon as possible after purification.
Conjugate with ReadiUse™ Preactivated APC-Cy7 Maleimide
- Reconstitute ReadiUse™ Preactivated APC-Cy7 Maleimide in 100 µL ddH2O to 10 mg/mL. Note: Reconstituted ReadiUse™ Preactivated APC-Cy7 Maleimide are stable at 4 °C for one week, please kept it from light.
- Add reduced antibody to pre-activated APC-Cy7 directly to at the ratio of 130 µg APC-Cy7 /100 µg reduced antibody.
- Rotate the mixture for 60-120 mins at room temperature.
- After 60 minutes, block the free sulfhydryls on the antibody.
- Prepare a fresh solution of 10 mg/mL NEM in DMSO; add 3.4 µL per mg of antibody and rotate for 20 minutes at room temperature.
Purification
- Antibody/APC-Cy7 conjugate could be further purified through size exclusion chromatography to get best performance. Note: The Antibody/APC-Cy7 conjugate solution is recommended to be stored at 2~8 °C and kept from light.
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Extinction coefficient (cm -1 M -1) | 700000 |
Excitation (nm) | 651 |
Emission (nm) | 779 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
ReadiUse™ Preactivated APC-Cy7 Tandem | 651 | 779 | 700000 |
ReadiUse™ Preactivated PE-Cy7 Maleimide | 565 | 778 | 1960000 |
ReadiUse™ Preactivated PerCP-Cy7 Maleimide | 482 | 784 | 350000 |
Images
References
View all 17 references: Citation Explorer
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Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080
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Journal: World journal of hepatology (2017): 1073-1080
Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.
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Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110
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Journal: Bulletin of experimental biology and medicine (2015): 238-42
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Journal: Journal of immunological methods (2013): 33-43
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Authors: Tölle, Angelika and Abdallah, Ziyad and Jung, Klaus and Bäumler, Hans
Journal: Journal of fluorescence (2010): 779-86
Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
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Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90
An optimized flow cytometry protocol for analysis of angiogenic monocytes and endothelial progenitor cells in peripheral blood.
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Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 848-53
Authors: Hristov, Mihail and Schmitz, Susanne and Schuhmann, Christoph and Leyendecker, Thorsten and von Hundelshausen, Philipp and Krötz, Florian and Sohn, Hae-Young and Nauwelaers, Frans A and Weber, Christian
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 848-53