Name: ReadiUse™ Preactivated APC-Cy7 Maleimide
Brand: AAT Bioquest
SKU: 2722
Price: 430 USD
Availability: InStock

ReadiUse™ Preactivated APC-Cy7 Maleimide

ReadiUse™ Preactivated APC-Cy7 Maleimide is a thiol-reactive fluorescent tandem dye for conjugating reduced antibodies and other biomolecules for multicolor flow cytometry.

Fluorescence Detection: Excitation at 651 nm and emission at 779 nm.
Maleimide Chemistry: SMCC-activated for thiol-reactive conjugation.
Ready-to-Use: Preactivated dye simplifies antibody conjugation.
Protein Compatibility: Suitable for reduced antibodies and other thiol-containing biomolecules.
Applications: Supports fluorescent antibody labeling and multicolor flow cytometry.

Flow cytometry analysis of whole blood stained with APC-Cy7 anti-human CD8 *HIT8a* conjugate. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the APC-Cy7 specific R7-A channel.

Protocol

SDS

Catalog	Size	Price	Quantity
2722	1 mg	Price

Overview

APC-Cy7 is a popular tandem color used in flow cytometry. Its primary absorption peak is at 651 nm with emission peak at ~780 nm. AAT Bioquest offers this preactivated APC-Cy7 to facilitate the APC-Cy7 tandem conjugations to reduced antibodies and other biomolecules that contain a thiol group. Our preactivated APC-Cy7 maleimide is prepared from the commonly used crosslinker SMCC, and ready to conjugate. Allophycocyanin (APC) is a phycobiliprotein isolated from Spirulina sp., a blue-green alga. Like other phycobiliproteins, APC is fluorescent, with an extremely high absorptivity and a high quantum efficiency. It is a protein which can be easily linked to antibodies and other proteins by conventional protein cross-linking techniques without altering its spectral characteristics.

References

View all 17 references: Citation Explorer

CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.

Authors:

Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge

Journal:

World journal of hepatology (2017): 1073-1080

Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.

Authors:

De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110

Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.

Authors:

Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J

Journal:

Bone marrow transplantation (2015): 348-53

Analysis of Populations of Memory T-Helper Cells Expressing CXCR3 and CCR6 Chemokine Receptors in Peripheral Blood of Patients with Chronic Viral Hepatitis C.

Authors:

Elezov, D S and Kudryavtsev, I V and Arsent'ev, N A and Basin, V V and Esaulenko, E V and Semenov, A V and Totolyan, A A

Journal:

Bulletin of experimental biology and medicine (2015): 238-42

A flow cytometric method for the analysis of macrophages in the vascular wall.

Authors:

Moore, Jeffrey P and Sakkal, Samy and Bullen, Michelle L and Kemp-Harper, Barbara K and Ricardo, Sharon D and Sobey, Christopher G and Drummond, Grant R

Journal:

Journal of immunological methods (2013): 33-43

Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	700000
Excitation (nm)	651
Emission (nm)	779

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure

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Page updated on November 4, 2026